Characterization of a Schwann cell neurite‐promoting activity that directs motoneuron axon outgrowth

Bolin, Laurel M.; Shooter, Eric M.

doi:10.1002/jnr.490370105

Cited by 18 publications

(6 citation statements)

References 68 publications

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“…Alternatively, it has been shown that similar dopaminergic activity is secreted by Schwann cells (Springer et al, 1990;Collier and Martin, 1993). Another activity for motoneuron axon growth has also been demonstrated in CM from adult rat Schwann cells (Bolin and Shooter, 1994). It is therefore possible that the immortalized Schwann cells established in this study also produce unidentified neurotrophic factor(s).…”

Section: 0 T T T G a T G A C G T C A T G G A T T T T A T T C A A G mentioning

confidence: 79%

Spontaneously immortalized adult mouse Schwann cells secrete autocrine and paracrine growth‐promoting activities

Watabe

Fukuda

Tanaka

et al. 1995

J of Neuroscience Research

108

128

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We established spontaneously immortalized Schwann cell lines from long-term cultures of adult mouse dorsal root ganglia and peripheral nerves. One of the cell lines, designated IMS32, responded to mitogenic stimuli by platelet-derived growth factor (PDGF)-BB, acidic and basic fibroblast growth factors (aFGF, bFGF), and transforming growth factors (TGF)-beta 1 and -beta 2, as determined by bromodeoxyuridine (BrdU) incorporation and double immunofluorescence for S100 and BrdU. Furthermore, conditioned media (CM) obtained from IMS32 cells showed mitogenic activity for both IMS32 cells and long-term cultured Schwann cells. Western blot analysis revealed TGF-beta-like molecule in the CM, and the activity was absorbed with anti-TGF-beta neutralizing antibody. Reverse transcription followed by polymerase chain reaction (RT-PCR) of IMS32 RNA revealed that these cells expressed TGF-beta 1, -beta 2, and -beta 3 transcripts. When rat pheochromocytoma PC12 cells were incubated with the CM, they developed neurite growth. Coculture of PC12 and IMS32 cells also showed neurite growth of PC12 cells. RNA transcripts of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT3), ciliary neurotrophic factor (CNTF), and glial cell line-derived neurotrophic factor (GDNF) were detected from IMS32 cells by RT-PCR. In these, we sequenced the mouse GDNF cDNA coding region and observed 97% and 90% homologies to corresponding rat and human cDNA sequences, respectively. These results indicate that the immortalized Schwann cell line mitotically responds to various growth factors and secretes autocrine and paracrine growth-promoting activities in vitro.

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Section: 0 T T T G a T G A C G T C A T G G A T T T T A T T C A A G mentioning

confidence: 79%

Spontaneously immortalized adult mouse Schwann cells secrete autocrine and paracrine growth‐promoting activities

Watabe

Fukuda

Tanaka

et al. 1995

J of Neuroscience Research

108

128

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“…Lindholm et al ., 1987 ;M . C. Brown et al, 1991), brain-derived neurotrophic factor (BDNF) (Acheson et al, 1991 ;Meyer et al, 1992), CNTF (Stockli et al ., 1989 ;Friedman et al ., 1992;Sendtner et al ., 1992), and LIF (Bolin and Shooter, 1994;Curtis et al, 1994) . The data presented here add IL-6 to the Schwann cell's isolated from iSC cells that were stimulated with LPS, TNF-a, IL-1a, serum withdrawal (as described in legend to Fig.…”

Section: Discussionmentioning

confidence: 99%

Interleukin‐6 Production by Schwann Cells and Induction in Sciatic Nerve Injury

Bolin

Verity

Silver

et al. 1995

Journal of Neurochemistry

241

126

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Interleukin‐6 (IL‐6) was produced by the spontaneously immortal Schwann cell clone, iSC, when cocultured with PC12 cells. The iSC cell‐derived IL‐6 in coculture conditioned media caused the neuronal differentiation of naive PC12 cells and this bioactivity was neutralized by preincubation of conditioned media with antisera to IL‐6. Cocultured iSC transcribe IL‐6 message as confirmed by northern analysis. Stimuli that induce IL‐6 production in the hematopoietic lineage induced transcription and production of IL‐6 by iSC cells. Lipopolysaccharide, tumor necrosis factor‐α, IL‐1α, IL‐6, and serum withdrawal induced iSC cell IL‐6 mRNA. The kinetics of IL‐6 production was confirmed in the mouse IL‐6‐dependent B9 bioassay and that activity could be neutralized with antisera to IL‐6. Expression of both the IL‐6 receptor and the gp130 signal transduction component by iSC as determined by northern analysis suggests an autocrine regulatory mechanism. The observed iSC production of IL‐6 in vitro led to an investigation of the sciatic nerve crush model of Schwann cell activation in vivo. In the initial 12 h after crush injury, IL‐6 message is induced. IL‐6 mRNA expression was highest distal to the crush injury. Our in vitro data demonstrate that iSC cells produce IL‐6 in response to PC12 cell coculture and to stimuli that induce IL‐6 production in the hematopoietic lineage. The induction of IL‐6 message distal to a crush injury suggests another mechanism by which Schwann cells facilitate peripheral nerve regeneration.

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“…This destruction is far more massive than that seen with any of the neurotrophic factor knock-out models that produced only limited death of specific and often small neuronal populations. This is not too surprising, because Schwann cells are a source of neurotrophic factors, including NGF, BDNF, NT-3, and GDNF (Funakoshi et al, 1993;Bolin and Shooter, 1994;Wetmore and Olson, 1995) and contact a far greater proportion of the axonal surface than do their targets. Because these factors induce the rapid release of NRG, it is possible that some of the neuronal cell death seen in the knock-outs occurred indirectly as a result of failed Schwann cell support.…”

Section: Rapid Axoglial Communication Through Schwann Cell Neurotrophmentioning

confidence: 99%

Rapid Axoglial Signaling Mediated by Neuregulin and Neurotrophic Factors

Esper¹,

Loeb²

2004

J. Neurosci.

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During peripheral nervous system development, Schwann cells are precisely matched to the axons that they support. This is mediated by axonal neuregulins that are essential for Schwann cell survival and differentiation. Here, we show that sensory and motor axons rapidly release heparin-binding forms of neuregulin in response to Schwann cell-derived neurotrophic factors in a dose-dependent manner. Neuregulin release occurs within minutes, is saturable, and occurs from axons that were isolated using a newly designed chamber slide apparatus. Although NGF and glial cell line-derived neurotrophic factor (GDNF) were the most potent neurotrophic factors to release neuregulin from sensory neurons, GDNF and BDNF were most potent for motor neurons and were the predominant neuregulin-releasing neurotrophic factors produced by cultured Schwann cells. Comparable levels of neuregulin could be released at a similar rate from neurons after protein kinase C activation with the phorbol ester, phorbol 12-myristate 13-acetate, which has also been shown to promote the cleavage and release of neuregulin from its transmembrane precursor. The rapid release of neuregulin from axons in response to Schwann cell-derived neurotrophic factors may be part of a spatially restricted system of communication at the axoglial interface important for proper peripheral nerve development, function, and repair.

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Characterization of a Schwann cell neurite‐promoting activity that directs motoneuron axon outgrowth

Cited by 18 publications

References 68 publications

Spontaneously immortalized adult mouse Schwann cells secrete autocrine and paracrine growth‐promoting activities

Spontaneously immortalized adult mouse Schwann cells secrete autocrine and paracrine growth‐promoting activities

Interleukin‐6 Production by Schwann Cells and Induction in Sciatic Nerve Injury

Rapid Axoglial Signaling Mediated by Neuregulin and Neurotrophic Factors

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