Characterization of a Schizosaccharomyces pombe morphological mutant altered in the galactomannan content

Ribas, Juan Carlos

doi:10.1016/0378-1097(91)90096-s

Cited by 26 publications

(19 citation statements)

References 14 publications

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“…A high amount of glucose was liberated by sulfuric acid treatment (about 80% of cell wall dry mass). Acid hydrolysis also released 10% of both galactose and mannose, most likely from the galactomannan structure present in this yeast species (Ribas et al, 1991), while only a tiny amount of glucosamine (less than 0·5%) could be detected, in agreement with previous data (Bush et al, 1974;Sietsma and Wessels, 1990). The cell walls were obtained from exponentially growing cells of JF291 cultivated on glucose (collected at 2 10 7 cells/ml).…”

Section: Comparison Of Three Acid Hydrolysis Procedures On Commercialsupporting

confidence: 91%

A new method for quantitative determination of polysaccharides in the yeast cell wall. Application to the cell wall defective mutants ofSaccharomyces cerevisiae

Dallies

François

Paquet

1998

Yeast

231

165

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Section: Comparison Of Three Acid Hydrolysis Procedures On Commercialsupporting

confidence: 91%

A new method for quantitative determination of polysaccharides in the yeast cell wall. Application to the cell wall defective mutants ofSaccharomyces cerevisiae

Dallies

François

Paquet

1998

Yeast

231

165

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“…A collection of S. pombe papulacandin B-resistant (26), and some of them are still susceptible to aculeacin A. In the present work with S. cerevisiae mutants, we have shown that resistance to aculeacin A does not necessarily correlate with resistance to papulacandin B, that is, there are common and specific steps in the mechanisms of entry and action of these antibiotics.…”

Section: Discussionmentioning

confidence: 50%

Isolation and characterization of Saccharomyces cerevisiae mutants resistant to aculeacin A

Mora

Gil

Sentandreu

et al. 1991

Antimicrob Agents Chemother

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Aculeacin A is a lipopeptide that inhibits ,B-glucan synthesis in yeasts. A number of Saccharomyces cerevisiae mutants resistant to this antibiotic were isolated, and four loci (ACRI, ACR2, ACR3, and ACR4) whose products are involved in the sensitivity to aculeacin A of yeast ceils were defined. Mutants containing mutations in the four loci were also resistant to echinocandin B, another member of this lipopeptide family of antibiotics. In contrast, acri, acr3, and acr4 mutants were resistant to papulacandin B (an antibiotic containing a disaccharide linked to two fatty acid chains that also inhibits P-glucan synthesis), but acr2 mutants were susceptible'to this antibiotic. This result defines common and specific steps in the entry and action of aculeacin A and papulacandin B. The analysis of double mutants revealed an epistatic effect of the acr2 mutation on the other three mutations. Cell walls of the four different mutants did not show significant alterations in composition with respect to the parental strain, and in vitro glucan synthase activity was also unaffected. However, cell surface hydrophobicity in three of the mutants was considerably decreased with respect to the parental strain.

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“…In a random spore analysis, all of them segregated haploids with wild-type morphology; thus their mutations were not allelic. The isolates were also tested for complementation with mutations in the genes cwg1, cwg2, orb1 to orb12, ral1 to ral4, sph1, and ste5(ras1) (see Table 1), which are known to make the cells more or less round or oval (6,7,37,38,51). To overcome sterility, protoplast fusion was used for hybridization.…”

Section: Isolation and Characterization Of Spherical Mutantsmentioning

confidence: 99%

Role of Cell Shape in Determination of the Division Plane in Schizosaccharomyces pombe : Random Orientation of Septa in Spherical Cells

Sipiczki

Yamaguchi

Grallert³

et al. 2000

J Bacteriol

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The establishment of growth polarity in Schizosaccharomyces pombe cells is a combined function of the cytoplasmic cytoskeleton and the shape of the cell wall inherited from the mother cell. The septum that divides the cylindrical cell into two siblings is formed midway between the growing poles and perpendicularly to the axis that connects them. Since the daughter cells also extend at their ends and form their septa at right angles to the longitudinal axis, their septal (division) planes lie parallel to those of the mother cell. To gain a better understanding of how this regularity is ensured, we investigated septation in spherical cells that do not inherit morphologically predetermined cell ends to establish poles for growth. We studied four mutants (defining four novel genes), over 95% of whose cells displayed a completely spherical morphology and a deficiency in mating and showed a random distribution of cytoplasmic microtubules, Tea1p, and F-actin, indicating that the cytoplasmic cytoskeleton was poorly polarized or apolar. Septum positioning was examined by visualizing septa and division scars by calcofluor staining and by the analysis of electron microscopic images. Freeze-substitution, freeze-etching, and scanning electron microscopy were used. We found that the elongated bipolar shape is not essential for the determination of a division plane that can separate the postmitotic nuclei. However, it seems to be necessary for the maintenance of the parallel orientation of septa over the generations. In the spherical cells, the division scars and septa usually lie at angles to each other on the cell surface. We hypothesize that the shape of the cell indirectly affects the positioning of the septum by directing the extension of the spindle.Cell polarization is a fundamental requirement of cell growth, division, and differentiation. The polar growth ensures directed cell extension, the correct organization of cytoskeletal structures, and the development of proper cell shape (for recent reviews, see references 8, 16, 23, 25, and 35) and orients the mitotic spindle (reviewed in references 22, 36, and 48). In most animal and plant species, the mitotic spindle then determines the plane of division (39,52). In the budding yeast Saccharomyces cerevisiae, the division plane is independent of spindle orientation because it is predetermined by the position of the bud (18). The division takes place at the narrow neck between the mother and bud cells. The site of bud formation is determined by the location of a previous one, and the axis of the spindle is oriented by directing cytoplasmic microtubules running from the spindle pole body into the bud (17, 18).

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Characterization of a Schizosaccharomyces pombe morphological mutant altered in the galactomannan content

Cited by 26 publications

References 14 publications

A new method for quantitative determination of polysaccharides in the yeast cell wall. Application to the cell wall defective mutants ofSaccharomyces cerevisiae

A new method for quantitative determination of polysaccharides in the yeast cell wall. Application to the cell wall defective mutants ofSaccharomyces cerevisiae

Isolation and characterization of Saccharomyces cerevisiae mutants resistant to aculeacin A

Role of Cell Shape in Determination of the Division Plane in Schizosaccharomyces pombe : Random Orientation of Septa in Spherical Cells

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