Characterization of a satellite DNA from Antilocapra americana

Denome, Roger; O’Callaghan, Brennon; Luitjens, Cameron; Harper, Elizabeth I.; Bianco, Robert A.

doi:10.1016/0378-1119(94)90016-7

Cited by 10 publications

(7 citation statements)

References 8 publications

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“…Short repeated submotifs have been described in many satellite DNA se- quences from different mammalian species, e.g. the satellite of the kangaroo rat [Hatch et al, 1976;Fry and Salser, 1977], the blue whale [Arnason and Widegren, 1989], the American pronghorn antelope [Denome et al, 1994], the mouse [Zhang and Horz, 1984], and some marsupials [Bulazel et al, 2006].…”

Section: Resultsmentioning

confidence: 99%

Molecular and Physical Characterization of the Complex Pericentromeric Heterochromatin of the Vole Species Microtus thomasi

Rovatsos

Marchal

Romero-Fernández

et al. 2014

Cytogenet Genome Res

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A new repeated DNA from Microtus thomasi, Mth-Alu2.2, was cloned and characterized and is presented here for the first time. Digestion of genomic DNA from M. thomasi with AluI restriction enzyme revealed a 2.2-kb repetitive DNA sequence with a high AT content (69%). This sequence consists of a tandemly repeated nonanucleotide of the consensus sequence CACAATGTA, which constitutes approximately 93-95% of the total unit length. The location of the Mth-Alu2.2 sequence in the karyotype was determined by FISH, demonstrating strong hybridization signals in the pericentromeric regions of all chromosomes and in the heterochromatin blocks of several X chromosome variants. In addition, the distribution of the 4 pericentromeric repeat sequences Msat-160, Mth-Alu900, Mth-Alu2.2, and interstitial telomeric repeats was analyzed by in situ hybridization in M. thomasi, in order to shed light on the complex composition of the chromosomal pericentromeric regions in this species. The order and organization of these sequences in the pericentromeric regions are conserved, with slight variations in both the degree of overlapping and the amount of each repeated DNA in the chromosomes. Specifically, Mth-Alu2.2 is localized in the terminal regions of the chromosomes, with Msat-160 occupying the immediately inner region, partially intermixed with Mth-Alu2.2. The sequence Mth-Alu900 is found in internal positions below Msat-160, and the interstitial telomeric repeats are located close to the long-arm euchromatin of the chromosomes.

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Section: Resultsmentioning

confidence: 99%

Molecular and Physical Characterization of the Complex Pericentromeric Heterochromatin of the Vole Species Microtus thomasi

Rovatsos

Marchal

Romero-Fernández

et al. 2014

Cytogenet Genome Res

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show abstract

“…Short repeated sub-motifs have been described in many satellite DNA sequences from different mammalian species, e.g. the satellite of the kangaroo rat (Hatch et al, 1976;Fry and Salser, 1977), the blue whale (Arnason and Widegren, 1989), the American pronghorn antelope (Denome et al, 1994), the mouse (Zhang and Horz, 1984) and some marsupials (Bulazel et al, 2006).…”

Section: Resultsmentioning

confidence: 99%

A new pericentromeric repeated DNA sequence in <i>Microtus thomasi</i>

Acosta

Marchal

Mitsainas

et al. 2009

Cytogenet Genome Res

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Several karyotypic forms have been previously described in populations of the vole species Microtus thomasi from Greece. In particular, the karyomorphs Microtus thomasi ‘thomasi’ and ‘atticus’ differ in X chromosome morphology, being acrocentric and subtelocentric, respectively. Furthermore, remarkable heterochromatin content variability has been described in sex chromosomes of both karyomorphs. Genomic DNA digestion with AluI allowed us to clone an 884 bp long repeated DNA sequence (Mth-Alu900) from the karyomorph M. thomasi ‘atticus’. This repeated DNA is AT rich and seems to be organized mainly as a dimer of the 884-bp unit, which presents three simple repeats (CAAAT, CAGAT and CAGAC) that constitute 80% of the total unit length. This repeated DNA is exclusive to M. thomasi, since it is absent from the genome of other studied Arvicolinae species. The chromosomal location of Mth-Alu900 was analyzed on M. thomasi ‘thomasi’ and M. thomasi ‘atticus’ karyomorphs, with different sex chromosome constitution. It was mainly located on the pericentromeric heterochromatin of most autosomes and X chromosomes on both karyomorphs. Results are also discussed in relation to karyotypic and sex chromosome variations in M. thomasi. To our knowledge, Mth-Alu900 constitutes a new – the third discovered so far – pericentromeric repeated DNA sequence described in Microtus species.

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“…A 31-mer motif is the subrepeat of bovine satellites I (Plucienniczak et al 1982) and 1.711b (Streeck 1982), ovine/caprine satellites I (Buckland 1983;Reisner and Bucholtz 1983;Novak 1984), deer satellites IA (Bogenberger et al 1987b), and a pronghorn satellite (Denome et al 1994). The bovine and ovine satellite I probes hybridize to each other and to the deer satellite, but not to DNA of giraffe and chevrotain.…”

Section: Satellitesmentioning

confidence: 99%

Evolution and recombination of bovine DNA repeats

et al. 1995

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The history of the abundant repeat elements in the bovine genome has been studied by comparative hybridization and PCR. The Bov-A and Bov-B SINE elements both emerged just after the divergence of the Camelidae and the true ruminants. A 31-bp subrepeat motif in satellites of the Bovidae species cattle, sheep, and goat is also present in Cervidae (deer) and apparently predates the Bovidae. However, the other components of the bovine satellites were amplified after the divergence of the cattle and the Caprinae (sheep and goat). A 23-bp motif, which as subrepeat of two major satellites occupies 5% of the cattle genome, emerged only after the split of the water buffalo and other cattle species. During the evolution of the Bovidae the satellite repeat units were shaped by recombination events involving subrepeats, other satellite components, and SINE elements. Differences in restriction sites of homologous satellites indicate a continuing rapid horizontal spread of new sequence variants.

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Characterization of a satellite DNA from Antilocapra americana

Cited by 10 publications

References 8 publications

Molecular and Physical Characterization of the Complex Pericentromeric Heterochromatin of the Vole Species Microtus thomasi

Molecular and Physical Characterization of the Complex Pericentromeric Heterochromatin of the Vole Species Microtus thomasi

A new pericentromeric repeated DNA sequence in <i>Microtus thomasi</i>

Evolution and recombination of bovine DNA repeats

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