Characterization of a Sapstaining Fungus,<i>Ophiostoma floccosum</i>, Isolated from the Sapwood of<i>Pinus thunbergii</i>in Korea

Yun, Yeo Hong; Hyun, Min Woo; Suh, Dong Yeon; Kim, Seong Hwan

doi:10.4489/myco.2009.37.1.005

Cited by 4 publications

(2 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fax: +82-41-523-3454. Currently, there has been no known molecular marker for O. floccosum, one of common blue stain species reported worldwide including Korea (Yun et al, 2009). In this study, we report a PCR-based molecular marker for differentiating O. floccosum from other blue stain fungi.…”

Section: Introductionmentioning

confidence: 92%

Development of PCR method for fast detection of Ophiostoma floccosum in wood chips

Yun¹,

Suh²,

Son³

et al. 2013

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

Ophiostoma floccosum is one of blue stain fungi that cause cosmetic damage in softwood. To develop a molecular maker for the detection of O. floccosum, we designed Oflo1 and Oflo2 primers based on the β-tubulin gene. PCR assay with eleven isolates of O. floccosum and nine other blue stain and discoloring fungal species (moulds) proved that the designed primers specifically amplified a 333 bp sized DNA band only from genomic DNA of O. floccosum isolates. Together with microwave heating extraction of spore DNA, we could detect O. floccosum within 2 h on artificially inoculated wood chip. We believed that the developed PCR method will be useful where detection of target species such as O. floccosum is required.

show abstract

Section: Introductionmentioning

confidence: 92%

Development of PCR method for fast detection of Ophiostoma floccosum in wood chips

Yun¹,

Suh²,

Son³

et al. 2013

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

show abstract

“…Primers used in this study. glutaraldehyde for 12 h and then 1% osmic acid for 1 h [19]. The fixed sample was washed with a 0.05 M cacodylate buffer and dehydrated by sequentially dipping for 30 min in each different ethanol concentration from 50% to 100%.…”

Section: Analysis Of Multiple Buddingmentioning

confidence: 99%

UmTco1, a Hybrid Histidine Kinase Gene, Is Essential for the Sexual Development and Virulence of Ustilago maydis

Yun¹,

Oh²,

Kim³

et al. 2017

Journal of Microbiology and Biotechnology

Self Cite

View full text Add to dashboard Cite

Hybrid histidine kinase is part of a two-component system that is required for various stress responses and pathogenesis of pathogenic fungi. The gene in human pathogen encodes a hybrid histidine kinase and is important for pathogenesis. In this study, we identified a homolog,, in the maize pathogen by bioinformatics analysis. To explore the role of in the survival of under environmental stresses and its pathogenesis, mutants were constructed by allelic exchange. The growth of mutants was significantly impaired when they were cultured under hyperosmotic stress. The mutants exhibited increased resistance to antifungal agent fludioxonil. In particular, the mutants were unable to produce cytokinesis or conjugation tubes, and to develop fuzzy filaments, resulting in impaired mating between compatible strains. The expression levels of, and , which are involved in the pheromone pathway, were significantly decreased in the mutants. In inoculation tests to the host plant, the mutants showed significantly reduced ability in the production of anthocyanin pigments and tumor development on maize leaves. Overall, the combined results indicated that plays important roles in the survival under hyperosmotic stress, and contributes to cytokinesis, sexual development, and virulence of by regulating the expression of the genes involved in the pheromone pathway.

show abstract

Ophiostoma haidaense , sp. nov., a new member of the O. piceae species complex associated with yellow-cedar, Callitropsis nootkatensis

Feau,

Tanney,

Herath

et al. 2024

Mycologia

View full text Add to dashboard Cite

Characterization of a Sapstaining Fungus,Ophiostoma floccosum, Isolated from the Sapwood ofPinus thunbergiiin Korea

Cited by 4 publications

References 27 publications

Development of PCR method for fast detection of Ophiostoma floccosum in wood chips

Development of PCR method for fast detection of Ophiostoma floccosum in wood chips

UmTco1, a Hybrid Histidine Kinase Gene, Is Essential for the Sexual Development and Virulence of Ustilago maydis

Ophiostoma haidaense , sp. nov., a new member of the O. piceae species complex associated with yellow-cedar, Callitropsis nootkatensis

Contact Info

Product

Resources

About