Characterization of a Saccharomyces cerevisiae mutant with enhanced production of β-d-fructofuranosidase

ul-Haq, Ikram; Ali, Sikander; Aslam, Aafia; Qadeer, M. A.

doi:10.1016/j.biortech.2006.11.047

Cited by 17 publications

(7 citation statements)

References 16 publications

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“…Such value was almost two times higher than that attained with cells immobilized on polyurethane foam, which provided the lowest enzyme activity. Although the production level of FFase depends to a great extent of the microorganism, basal substrate, and the microbial production process, 31 the results achieved by using vegetal fiber as immobilization carrier can be favorably compared with those obtained during FOS production by other fungus strains. For example, under optimized cultivation conditions, A. japonicus JN-19 yielded a FFase activity of 55.42 U/mL after 96 h fermentation, and the FOS production yield was 55.8%.…”

Section: B-fructofuranosidase Activitymentioning

confidence: 99%

“…For example, under optimized cultivation conditions, A. japonicus JN-19 yielded a FFase activity of 55.42 U/mL after 96 h fermentation, and the FOS production yield was 55.8%. 32 By using a mutant Saccharomyces cerevisiae and optimized fermentation conditions, ul-Haq et al 31 produced FFase with a specific activity of 45.65 U/mL, after 48 h incubation time. In the present study, a FFase activity of 42.9 U/mL was obtained only after 36 h fermentation, giving a higher productivity, and the FOS yield was 69%.…”

Section: B-fructofuranosidase Activitymentioning

confidence: 99%

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Colonization of Aspergillus japonicus on synthetic materials and application to the production of fructooligosaccharides

Mussatto

Aguilar

Rodrigues

et al. 2009

Carbohydrate Research

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The ability of Aspergillus japonicus ATCC 20236 to colonize different synthetic materials (polyurethane foam, stainless steel sponge, vegetal fiber, pumice stones, zeolites, and foam glass) and to produce fructooligosaccharides (FOS) from sucrose (165 g/L) is described. Cells were immobilized in situ by absorption, through direct contact with the carrier particles at the beginning of fermentation. Vegetal fiber was the best immobilization carrier as A. japonicus grew well on it (1.25 g/g carrier), producing 116.3g/L FOS (56.3g/L 1-kestose, 46.9 g/L 1-nystose, and 13.1g/L 1-beta-fructofuranosyl nystose) with 69% yield (78% based only in the consumed sucrose amount), giving also elevated activity of the beta-fructofuranosidase enzyme (42.9 U/mL). In addition, no loss of material integrity, over a 2 day-period, was found. The fungus also immobilized well on stainless steel sponge (1.13 g/g carrier), but in lesser extents on polyurethane foam, zeolites, and pumice stones (0.48, 0.19, and 0.13 g/g carrier, respectively), while on foam glass no cell adhesion was observed. When compared with the FOS and beta-fructofuranosidase production by free A. japonicus, the results achieved using cells immobilized on vegetal fiber were closely similar. It was thus concluded that A. japonicus immobilized on vegetal fiber is a potential alternative for high production of FOS at industrial scale.

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Section: B-fructofuranosidase Activitymentioning

confidence: 99%

Section: B-fructofuranosidase Activitymentioning

confidence: 99%

Colonization of Aspergillus japonicus on synthetic materials and application to the production of fructooligosaccharides

Mussatto

Aguilar

Rodrigues

et al. 2009

Carbohydrate Research

View full text Add to dashboard Cite

show abstract

“…Chitinase production was improved by sixfold in Ophiostoma floccosum using UV mutagenesis [30]. Furthermore, the ethyl methanesulfonate (EMS) mutant with an enzyme activity of 25.56 U ml −1 was obtained by further exposure to UV radiation and yielded an activity of 34.12 U ml −1 [31].…”

Section: Improvement Of the Bioagents Via Mutagenesismentioning

confidence: 99%

Application of Radiation and Genetic Engineering Techniques to Improve Biocontrol Agent Performance: A Short Review

Mirmajlessi¹,

Mostafavi²,

Loit³

et al. 2019

Use of Gamma Radiation Techniques in Peaceful Applications

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Biological control is a potential nonchemical method to manage plant pathogens by beneficial microorganisms. To improve antagonistic potential of biocontrol agents, mutation by radiations, chemicals, and genetic manipulations has been used. Genetic techniques and ionizing radiation containing direct or indirect emissions play the greatest role for selection of useful microorganisms to enhance the efficiency of biological systems. Indeed, genetic engineering has a main role in increasing antimicrobial metabolites, host colonization ability, and endurance in micro-ecosystem. Genetic improvement can be achieved by protoplast fusion, genetic modification (GM), and chemical (genotoxic agents) and physical mutations. However, ultraviolet light and ionizing radiations can induce modifications in the genome of an organism. Irradiation, particularly gamma rays, is also applied for controlling postharvest diseases. Indeed, irradiation cannot completely eliminate pathogens, but it might result in cell injury and directly damage the chromosomal DNA of a living cell. This technology has been used for many reasons including disinfestation of foods, reducing foodborne pathogens, and extending shelf life many fruits, vegetables, and nuts. In the current review, we discuss advances in the radiation and molecular genetic techniques with the aim to improve antagonistic potential of microorganisms as it is applied to the suppression of plant pathogens.

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“…Nowadays, biomass materials have been successfully applied in the polymer composites, such as wood plastic composites and wood elastomer composites , which not only can make the full advantages of biomass materials, such as lower density, less expense, renewable and biodegradable , but also have the unique advantage of polymer composites like high mechanical performance, hydrophobicity and anti‐rot properties. However, there has been limited studies utilizing natural fiber to make rubber composites , especially no study was found to use straw fiber for rubber composites. When natural fiber was incorporated into rubber, some properties such as damping vibration, indoor sound insulation and absorbing, electrical insulation, etc., were improved and its application was broadened.…”

Section: Introductionmentioning

confidence: 99%

Comparison on the properties of the wheat straw/rubber composites by adding graphite/carbon fiber

2018

Polymer Composites

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The conductive wheat straw/rubber composites (WSRCs) were prepared with wheat straw fiber (WSF), rubber compound and graphite powder (GP), or carbon fiber (CF). WSRCs with different loading of conductive fillers were prepared. The curing properties, mechanical properties, dynamic mechanical properties, morphology structure and conductive properties of the composites were investigated with reference to the conductive filler loading. Sodium hydroxide combined with bis‐[γ‐(triethoxysily)‐propyl] tetrasulfide (NaOH‐Si69‐treated WSF) had a better bonding with the rubber matrix. The incorporation of GP and CF could restrict the mobility of matrix macromolecular chains and increase the viscosity of GP/CF‐filled WSRCs, resulting in the increases in torque, stiffness and storage modulus and the slight reduction in scorch time and tensile strength as well as the damping behavior. Moreover, the conductivity of the WSRCs was increased significantly as the increase in filler loading. It was also found that the conductivity of GP‐filled WSRCs were higher than that of the CF‐filled ones. POLYM. COMPOS., 40:E983–E992, 2019. © 2018 Society of Plastics Engineers

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Characterization of a Saccharomyces cerevisiae mutant with enhanced production of β-d-fructofuranosidase

Cited by 17 publications

References 16 publications

Colonization of Aspergillus japonicus on synthetic materials and application to the production of fructooligosaccharides

Colonization of Aspergillus japonicus on synthetic materials and application to the production of fructooligosaccharides

Application of Radiation and Genetic Engineering Techniques to Improve Biocontrol Agent Performance: A Short Review

Comparison on the properties of the wheat straw/rubber composites by adding graphite/carbon fiber

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