Characterization of a recombinant xylose tolerant β-xylosidase from Humicola grisea var. thermoidea and its use in sugarcane bagasse hydrolysis

Cintra, Lorena Cardoso; Fernandes, Amanda Gregorim; Oliveira, Izadora Cristina Moreira de; Siqueira, Saulo José Linhares de; Costa, Ilítia Ganaê Oliveira; Colussi, Francieli; Jesuino, Rosália Santos Amorim; Ulhôa, Cirano José; Faria, Fabrícia Paula de

doi:10.1016/j.ijbiomac.2017.07.039

Cited by 28 publications

(11 citation statements)

References 48 publications

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“…Activity of the purified enzymes was determined using p-nitrophenyl glycosides (Supplementary Table 7). The specific activity of β-Xylp_1710 is one of the highest reported for β-xylosidases [31][32][33][34][35] while the measured activities on pNP α-L-arabinofuranoside were up to 100-fold lower than others 31,36 . There have been very few α-xylosidases described 37 .…”

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confidence: 80%

Development of a thermophilic coculture for corn fiber conversion to ethanol

et al. 2020

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The fiber in corn kernels, currently unutilized in the corn to ethanol process, represents an opportunity for introduction of cellulose conversion technology. We report here that Clostridium thermocellum can solubilize over 90% of the carbohydrate in autoclaved corn fiber, including its hemicellulose component glucuronoarabinoxylan (GAX). However, Thermoanaerobacterium thermosaccharolyticum or several other described hemicellulose-fermenting thermophilic bacteria can only partially utilize this GAX. We describe the isolation of a previously undescribed organism, Herbinix spp. strain LL1355, from a thermophilic microbiome that can consume 85% of the recalcitrant GAX. We sequence its genome, and based on structural analysis of the GAX, identify six enzymes that hydrolyze GAX linkages. Combinations of up to four enzymes are successfully expressed in T. thermosaccharolyticum. Supplementation with these enzymes allows T. thermosaccharolyticum to consume 78% of the GAX compared to 53% by the parent strain and increases ethanol yield from corn fiber by 24%.

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confidence: 80%

Development of a thermophilic coculture for corn fiber conversion to ethanol

et al. 2020

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“…The barley brewing residue was the most efficient at producing β-Xylosidase under stirring conditions compared to the other carbon sources; the differences between these residues were very constrained. However, the residue from the brewing industry was very efficient in inducing improved production of β-Xylosidase between different carbon sources tested under the conditions of stationary liquid culture, providing a maximum enzyme activity of 15.45 U mL applications such as thermostability, high tolerance xylose and saccharification of biomass by convert xylan into fementable monosaccharides [16,17]. Michelin et al [18] conducted experiments on the induction of β-Xylosidase activity of the fungi A. ochraceus and A. terricola, and enzymatic activity levels of 30 U mL -1 and 56 U mL -1 , respectively, were obtained in the presence of another agroresidue that is very abundant in North America: corn cob.…”

Section: Resultsmentioning

confidence: 99%

Experimental Design for Optimization of β-Xylosidase Production by A. fumigatus Isolated from the Atlantic Forest (Brazil)

Vieira¹,

Christ²,

Graciano³

et al. 2019

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The production of β-Xylosidase by a new strain of Aspergillus fumigatus (PC-7S-2 M), isolated from the Brazilian Atlantic Forest, was analyzed at 28°C using modified Czapeck media supplemented with different agroindustrial residues at 1% (w/v). Conidia were inoculated for 7 days, and the best activity for β- Xylosidase was obtained in the presence of barley brewing residue after 4 days (15 U mL-1). To optimize the production of β-Xylosidase, this carbon source was used for a central composite rotational design (CCRD) to obtain a significance level of p < 0.10, which predicted an enzyme activity of 245.04 U mL-1. The model validation revealed β-Xylosidase activity was optimized at 229.06 U mL-1. Furthermore, the production of intracellular A. fumigatus β-Xylosidase increased by 1,500% (15 times) over that initially obtained, achieving 93.47% of the predicted model. This finding obtained during two years emphasizes the availability of A. fumigatus β-Xylosidase production with possible applications in biotechnological processes.

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“…The effects of metal ions, chelating agents, or surfactant solutions on the r-XynBS27 activity were determined after the enzyme had been incubated with the reagents at final concentration of 5 mM and pH 6.0. The effect of xylose on r-XynBS27 activity was evaluated after preincubation of the enzyme with different concentrations (40, 80 and 160 mM) of these compounds as described elsewhere [ 22 ]. After 60 min incubation at 50°C, the activity of the r-XynBS27 was determined using beechwood xylan as substrate.…”

Section: Methodsmentioning

confidence: 99%

Improvement of bread making quality by supplementation with a recombinant xylanase produced by Pichia pastoris

et al. 2018

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Xylanases (EC 3.2.1.8) are hydrolytic enzymes, which randomly cleave the β-1,4-linked xylose residues from xylan. The synthetic gene xynBS27 from Streptomyces sp. S27 was successfully cloned and expressed in Pichia pastoris. The full-length gene consists of 729 bp and encodes 243 amino acids including 51 residues of a putative signal peptide. This enzyme was purified in two steps and was shown to have a molecular weight of 20 kDa. The purified r-XynBS27 was active against beechwood xylan and oat spelt xylan as expected for GH 11 family. The optimum pH and temperature values for the enzyme were 6.0 and 75 °C, respectively. The Km and Vmax were 12.38 mg/mL and 13.68 μmol min/mg, respectively. The r-XynBS27 showed high xylose tolerance and was inhibited by some metal ions and by SDS. r-XynBS27 was employed as an additive in the bread making process. A decrease in firmness, stiffness and consistency, and improvements in specific volume and reducing sugar content were recorded.

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Characterization of a recombinant xylose tolerant β-xylosidase from Humicola grisea var. thermoidea and its use in sugarcane bagasse hydrolysis

Cited by 28 publications

References 48 publications

Development of a thermophilic coculture for corn fiber conversion to ethanol

Development of a thermophilic coculture for corn fiber conversion to ethanol

Experimental Design for Optimization of β-Xylosidase Production by A. fumigatus Isolated from the Atlantic Forest (Brazil)

Improvement of bread making quality by supplementation with a recombinant xylanase produced by Pichia pastoris

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