“…For instance, NCTC2472 bone cancers cells, MRMT-1 breast cancer cells, Lewis lung cancer cells, melanoma cancer cells, and prostate cancer cells have been employed to set up the animal model ( De Ciantis et al, 2010 ; Donovan-Rodriguez, Dickenson & Urch, 2005 ; Dore-Savard et al, 2010 ; Mao-Ying et al, 2006 ; Medhurst et al, 2002 ; Zhang & Lao, 2012 ). In our present study, our results showed that the values of pain behavior scoring were changed significantly on day 7 to day 9 in the K562 cells transplanted group, which is consistent with the behavioral changes of bone cancer pain in animal models transplanted with solid tumor cells ( De Ciantis et al, 2010 ; Dore-Savard et al, 2010 ; Mao-Ying et al, 2006 ; Medhurst et al, 2002 ; Zhang & Lao, 2012 ). To our knowledge, this is the first to employ leukemia cancer cells to build the bone pain animal model of leukemia, with minimal invasion in the surgery.…”