Characterization of a radioimmunoassay for ovine FSH utilizing an anti-bovine FSH serum

Cheng, K. W.; Simaraks, S.; Palmer, W. M.

doi:10.1530/jrf.0.0610115

Cited by 23 publications

(8 citation statements)

References 15 publications

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“…Double-antibody RIAs were used to determine serum concentrations of PRL (Sanford et al 1978), FSH (Cheng et al 1981), LH (Niswender et al 1969, Sanford 1987, and testosterone (Sanford 1985). RPs in the pituitary hormone assays were NIHoPRL-S12, NIH-oFSH-S12 and NIH-oLH-S14.…”

Section: Hormone Assays and Secretion Parametersmentioning

confidence: 99%

Prolactin regulation of testosterone secretion and testes growth in DLS rams at the onset of seasonal testicular recrudescence

Sanford

Baker²

2010

Reproduction

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Our objective was to test the hypothesis that prolactin (PRL) acts at both the pituitary and testis levels to regulate testosterone secretion in the adult ram. The focus was on the mid-regression to mid-redevelopment stages of a photoperiod-condensed 'seasonal' testicular cycle. DLS rams (six per group) were given daily s.c. injections of bromocriptine (4 mg) or vehicle during the entire period. Serum PRL concentration in control rams peaked at 103.4G22.1 ng/ml in late regression and then steadily declined (P!0.01) to 19.5G4.3 ng/ml, whereas PRL in treated rams was always %4.0 ng/ml. Suppression of PRL tended (P!0.10) to increase the amplitude of natural LH pulses (transition stages) or reduce the number of LH receptors in the testis (regressed stage), although neither change disturbed testosterone levels in peripheral blood. These subtle changes were accompanied by significant (P!0.05) alterations in the capability of the pituitary to release LH (85% more) and of the testes to secrete testosterone (20% less). These effects of PRL were unmasked when rams were given highly stimulative i.v. injections of GNRH (single 3 mg dose) and NIH-oLH-S24 (three 5 mg doses given 20 min apart) respectively. PRL insufficiency also appeared to slow down the 'seasonal' rise in FSH secretion and slightly delayed (2 weeks) the times when the testes began to grow and were first significantly (P!0.05) enlarged from the regressed state. We conclude that PRL is an important part of the intricate regulation of the pituitary-gonadal system in moderately seasonal DLS rams.

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Section: Hormone Assays and Secretion Parametersmentioning

confidence: 99%

Prolactin regulation of testosterone secretion and testes growth in DLS rams at the onset of seasonal testicular recrudescence

Sanford

Baker²

2010

Reproduction

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“…Follicle-stimulating hormone (FSH) was assayed by the RIA method of Cheng, Simaraks & Palmer (1981). Sensitivity was <2 µg/l using NIH-FSH-S15 as standard.…”

Section: Introductionmentioning

confidence: 99%

Effects of melatonin implants in ram lambs

Kennaway¹,

Gilmore²

1985

Reproduction

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“…Blood testosterone increases to or not transient increases in gonadotropin and supraphysiological levels following either (or) testosterone secretion would alter the active, chronic (Schanbacher 1984) or passive, pattern of testicular redevelopment and early acute (Sanford 1985) (Niswender et al 1969;Howland 1972), FSH (Cheng et al 1981) and prolactin (Sanford et al 198) …”

mentioning

confidence: 99%

Seasonal Variation in Circulating Gonadotropin and Testosterone Levels and in Testis Size of Yearling Rams During and Subsequent to Immunoneutralization of Estradiol or Tamoxifen Treatment in the Nonbreeding Season

Sanford¹

1987

Can. J. Anim. Sci.

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Characterization of a radioimmunoassay for ovine FSH utilizing an anti-bovine FSH serum

Abstract: Summary. The

Cited by 23 publications

References 15 publications

Prolactin regulation of testosterone secretion and testes growth in DLS rams at the onset of seasonal testicular recrudescence

Prolactin regulation of testosterone secretion and testes growth in DLS rams at the onset of seasonal testicular recrudescence

Effects of melatonin implants in ram lambs

Seasonal Variation in Circulating Gonadotropin and Testosterone Levels and in Testis Size of Yearling Rams During and Subsequent to Immunoneutralization of Estradiol or Tamoxifen Treatment in the Nonbreeding Season

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