Characterization of a Putative Membrane Receptor for Progesterone in Rat Granulosa Cells1

Peluso, John J.; Fernández, Gustavo; Pappalardo, Andrea; White, Bruce A.

doi:10.1095/biolreprod65.1.94

Cited by 62 publications

(54 citation statements)

References 37 publications

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“…These receptors are considered either the classical steroid receptors temporarily associated with membrane proteins, or unidentifi ed transmembrane steroid receptors. Many biological effects, including those of estrogen and progesterone in the female reproductive organs and in the brain, have been interpreted in terms of the nongenomic steroid action (Zhu and Pfaff 1998;Peluso et al 2001). In regard to androgens, recent studies have revealed the mechanisms of their nongenomic action in different systems.…”

Section: Discussionmentioning

confidence: 99%

Coexpression of Menin and JunD during the Duct Cell Differentiation in Mouse Submandibular Gland

Hipkaeo

Sakulsak

Wakayama

et al. 2008

Tohoku J. Exp. Med.

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In the submandibular gland (SMG) of mice, a duct portion called the granular convoluted tubule (GCT) is developed preferentially in males with puberty. This sexual dimorphism is androgen-dependent, but the underlying molecular mechanisms are unclear. We have demonstrated that the expression of a transcription factor JunD is regulated in association with the androgen-induced differentiation of GCT cells from striated duct (SD) cells. Menin, a nuclear protein encoded by the MEN1 tumor-suppressor gene, is known to bind JunD, thereby inhibiting its activity. In the present study, we examined the expression of menin in the mouse SMG by use of Northern blotting, Western blotting, and immunohistochemistry. Immunoreactivity for menin was higher in the female than male gland, and localized to the nuclei of intercalated duct cells and a subpopulation of SD cells. In contrast, GCT cells in males appeared negative for menin. The levels of menin in the SMG were increased with castration in males and decreased by repeated administration of testosterone to females or to castrated males. After a single administration of testosterone to females, many SD cells newly gained nuclear menin, which was lost as the cells converted to GCT cells by 48 hrs. These patterns of the expression and localization of menin were quite similar to those of JunD. Furthermore, the coimmunoprecipitation analysis of the SMG homogenates indicated that menin binds JunD in vivo. The present study suggests that the JunD-menin complex plays signifi cant roles in the androgen-dependent differentiation of the duct system in the mouse SMG.

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Section: Discussionmentioning

confidence: 99%

Coexpression of Menin and JunD during the Duct Cell Differentiation in Mouse Submandibular Gland

Hipkaeo

Sakulsak

Wakayama

et al. 2008

Tohoku J. Exp. Med.

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“…It is not likely that this action is mediated through the classic nuclear PR, since this protein does not seem to be expressed in these cells (363). Alternatively, a 60-kDa membrane protein has been detected in granulosa cells by the use of an antibody directed against the steroid binding domain of nuclear PR (c-262) (365).…”

Section: Progesteronementioning

confidence: 99%

“…Alternatively, a 60-kDa membrane protein has been detected in granulosa cells by the use of an antibody directed against the steroid binding domain of nuclear PR (c-262) (365). In ligand blot and ligand binding studies, it has been shown that this protein specifically binds progesterone (363).…”

Section: Progesteronementioning

confidence: 99%

Nongenomic Steroid Action: Controversies, Questions, and Answers

Lösel

Falkenstein

Feuring

et al. 2003

Physiological Reviews

500

318

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Lösel, Ralf M., Elisabeth Falkenstein, Martin Feuring, Armin Schultz, Hanns-Christian Tillmann, Karin Rossol-Haseroth, and Martin Wehling. Nongenomic Steroid Action: Controversies, Questions, and Answers. Physiol Rev 83: 965–1016, 2003; 10.1152/physrev.00003.2003.—Steroids may exert their action in living cells by several ways: 1) the well-known genomic pathway, involving hormone binding to cytosolic (classic) receptors and subsequent modulation of gene expression followed by protein synthesis. 2) Alternatively, pathways are operating that do not act on the genome, therefore indicating nongenomic action. Although it is comparatively easy to confirm the nongenomic nature of a particular phenomenon observed, e.g., by using inhibitors of transcription or translation, considerable controversy exists about the identity of receptors that mediate these responses. Many different approaches have been employed to answer this question, including pharmacology, knock-out animals, and numerous biochemical studies. Evidence is presented for and against both the participation of classic receptors, or proteins closely related to them, as well as for the involvement of yet poorly understood, novel membrane steroid receptors. In addition, clinical implications for a wide array of nongenomic steroid actions are outlined.

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“…Several types of G-protein-coupled progesterone membrane receptors have been identified in the rat ovary. These include PRMC1, PMRa, PMRb, PMRg, and RDA288 (Peluso & Pappalardo 1998, Peluso & Fernandez 2001, Bramley 2003, Peluso & Pappalardo 2004, Cai & Stocco 2005.…”

Section: Introductionmentioning

confidence: 99%

Interactions between progesterone and tumor necrosis factor-α in the regulation of primordial follicle assembly

Nilsson

Stanfield²,

Skinner

2006

Reproduction

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Follicle assembly is the process by which groups or 'nests' of oocytes break down to form primordial follicles. The size of the primordial follicle pool is the major determinant of the reproductive lifespan of a female. Previously, progesterone (P 4 ) has been shown to inhibit follicle assembly, while tumor necrosis factor-a (TNFa) has been shown to promote the apoptosis that is necessary for follicle assembly. The present study examines how TNFa and progesterone interact to regulate primordial follicle assembly. Ovaries were collected from newborn rats and placed in organ culture to examine the actions of P 4 and TNFa. P 4 was found to decrease primordial follicle assembly and increase the percentage of unassembled oocytes both in vitro and in vivo. TNFa treatment did not change the proportion of assembled follicles in cultured ovaries, but blocked the ability of P 4 to inhibit follicle assembly. Microarray analysis of the ovarian transcriptome revealed that progesterone treatment of the ovaries altered the expression of 513 genes with 132 only expressed after P 4 treatment and 16 only expressed in control ovaries. The majority of genes were up-regulated greater than twofold over control, with a small subset of 16 genes down-regulated. Categories of genes affected by P 4 are described including a group of extracellular signaling factors. The progesterone receptors expressed at the time of follicle assembly included the surface membrane progesterone receptors PGRMC1, PGRMC2, and RDA288. The nuclear genomic P 4 receptor was not expressed at appreciable levels. Progesterone increased the expression of several genes (TANK, NFkB, Bcl2l1, and Bcl2l2) involved in a signaling pathway that promotes cell survival and inhibits apoptosis. Observations indicate that P 4 acts through the surface membrane progesterone receptors to regulate primordial follicle assembly, and that TNFa can override the inhibitory actions of P 4 on follicle assembly. A major mechanism involved in the actions of P 4 is an increase in cell survival genes and inhibition of the apoptosis pathway. Observations provide insight into the hormonal regulation of primordial follicle assembly and lead to novel approaches to potentially manipulate follicle assembly and reproductive capacity.

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Characterization of a Putative Membrane Receptor for Progesterone in Rat Granulosa Cells1

Cited by 62 publications

References 37 publications

Coexpression of Menin and JunD during the Duct Cell Differentiation in Mouse Submandibular Gland

Coexpression of Menin and JunD during the Duct Cell Differentiation in Mouse Submandibular Gland

Nongenomic Steroid Action: Controversies, Questions, and Answers

Interactions between progesterone and tumor necrosis factor-α in the regulation of primordial follicle assembly

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