Characterization of a putative grapevine Zn transporter, VvZIP3, suggests its involvement in early reproductive development in Vitis vinifera L

Gainza, Felipe; Pérez-Díaz, Ricardo; Pérez-Castro, Ramón; Tapia, Jaime; Casaretto, José A.; González, Sebastián A.; Peña‐Cortés, Hugo; Ruíz-Lara, Simón; González, Enrique

doi:10.1186/1471-2229-12-111

Cited by 47 publications

(46 citation statements)

References 54 publications

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“…Interestingly, NsylIRT3 and NtomIRT3 transcripts are more abundant in flower tissues most likely for the redistribution of Zn and Fe. The function of Nicotiana IRT3 is possibly closer to the Zrt/IRT-like protein AtZIP4 , which is highly expressed in anther and pollen [61], where it is suspected to play a role in Zn redistribution in flowers ([62] and references therein). Thus, Zn and iron uptake is likely driven by AtIRT1 and AtIRT2 orthologous proteins in N. sylvestris , whereas another gene(s) is likely to perform this function in N. tomentosiformis .…”

Section: Resultsmentioning

confidence: 99%

Reference genomes and transcriptomes of Nicotiana sylvestris and Nicotiana tomentosiformis

et al. 2013

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BackgroundNicotiana sylvestris and Nicotiana tomentosiformis are members of the Solanaceae family that includes tomato, potato, eggplant and pepper. These two Nicotiana species originate from South America and exhibit different alkaloid and diterpenoid production. N. sylvestris is cultivated largely as an ornamental plant and it has been used as a diploid model system for studies of terpenoid production, plastid engineering, and resistance to biotic and abiotic stress. N. sylvestris and N. tomentosiformis are considered to be modern descendants of the maternal and paternal donors that formed Nicotiana tabacum about 200,000 years ago through interspecific hybridization. Here we report the first genome-wide analysis of these two Nicotiana species.ResultsDraft genomes of N. sylvestris and N. tomentosiformis were assembled to 82.9% and 71.6% of their expected size respectively, with N50 sizes of about 80 kb. The repeat content was 72-75%, with a higher proportion of retrotransposons and copia-like long terminal repeats in N. tomentosiformis. The transcriptome assemblies showed that 44,000-53,000 transcripts were expressed in the roots, leaves or flowers. The key genes involved in terpenoid metabolism, alkaloid metabolism and heavy metal transport showed differential expression in the leaves, roots and flowers of N. sylvestris and N. tomentosiformis.ConclusionsThe reference genomes of N. sylvestris and N. tomentosiformis represent a significant contribution to the SOL100 initiative because, as members of the Nicotiana genus of Solanaceae, they strengthen the value of the already existing resources by providing additional comparative information, thereby helping to improve our understanding of plant metabolism and evolution.

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Section: Resultsmentioning

confidence: 99%

Reference genomes and transcriptomes of Nicotiana sylvestris and Nicotiana tomentosiformis

et al. 2013

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“…The results showed that the ZmIRT1 , ZmZIP4 , ZmZIP5 and ZmZIP 6 contain cis -elements for seed expression (Additional file 7). Considering that there was some correlation between the VvZIP3 expression profile and the Zn accumulation pattern during the development of reproductive organs [42], we assumed that ZmIRT1 , ZmZIP4 , ZmZIP5 and ZmZIP6 may play various roles in both embryo and endosperm development.…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of the zinc-regulated transporters, iron-regulated transporter-like protein (ZIP) gene family in maize

et al. 2013

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BackgroundZinc (Zn) and iron (Fe) are essential micronutrients for plant growth and development, their deficiency or excess severely impaired physiological and biochemical reactions of plants. Therefore, a tightly controlled zinc and iron uptake and homeostasis network has been evolved in plants. The Zinc-regulated transporters, Iron-regulated transporter-like Proteins (ZIP) are capable of uptaking and transporting divalent metal ion and are suggested to play critical roles in balancing metal uptake and homeostasis, though a detailed analysis of ZIP gene family in maize is still lacking.ResultsNine ZIP-coding genes were identified in maize genome. It was revealed that the ZmZIP proteins share a conserved transmembrane domain and a variable region between TM-3 and TM-4. Transiently expression in onion epidermal cells revealed that all ZmZIP proteins were localized to the endoplasmic reticulum and plasma membrane. The yeast complementation analysis was performed to test the Zn or Fe transporter activity of ZmZIP proteins. Expression analysis showed that the ZmIRT1 transcripts were dramatically induced in response to Zn- and Fe-deficiency, though the expression profiles of other ZmZIP changed variously. The expression patterns of ZmZIP genes were observed in different stages of embryo and endosperm development. The accumulations of ZmIRT1 and ZmZIP6 were increased in the late developmental stages of embryo, while ZmZIP4 was up-regulated during the early development of embryo. In addition, the expression of ZmZIP5 was dramatically induced associated with middle stage development of embryo and endosperm.ConclusionsThese results suggest that ZmZIP genes encode functional Zn or Fe transporters that may be responsible for the uptake, translocation, detoxification and storage of divalent metal ion in plant cells. The various expression patterns of ZmZIP genes in embryo and endosperm indicates that they may be essential for ion translocation and storage during differential stages of embryo and endosperm development. The present study provides new insights into the evolutionary relationship and putative functional divergence of the ZmZIP gene family during the growth and development of maize.

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“…The endogenous phytoene desaturase (PDS) gene and the magnesium chelatase subunit gene (Chl1) were both used as endogenous reporters to validate constructs inducing gene silencing in N. benthamania and tobacco plants (Golenberg et al, 2009;Ruiz et al, 1998). Inactivation of these genes results in loss of chlorophyll, leading to visible leaf bleaching.…”

Section: Endogenous Plant Genes As Silencing Reportersmentioning

confidence: 99%

“…Likewise, the ATP-binding cassette protein ABCC1 was localized to the tonoplast (Francisco et al, 2013). Particle bombardment of onion cells can also help investigate the localization of grapevine proteins, as shown for the zinc transporter ZIP3 in the plasma membrane (Gainza-Cort es et al, 2012). However, gene expression in heterologous systems may exhibit aberrant traits, presumably due to a foreign genetic background.…”

Section: Introductionmentioning

confidence: 99%

Transient expression assays in grapevine: a step towards genetic improvement

Jelly

Valat

Walter

et al. 2014

Plant Biotechnology Journal

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SummaryIn the past few years, the usefulness of transient expression assays has continuously increased for the characterization of unknown gene function and metabolic pathways. In grapevine (Vitis vinifera L.), one of the most economically important fruit crops in the world, recent systematic sequencing projects produced many gene data sets that require detailed analysis. Due to their rapid nature, transient expression assays are well suited for large-scale genetic studies. Although genes and metabolic pathways of any species can be analysed by transient expression in model plants, a need for homologous systems has emerged to avoid the misinterpretation of results due to a foreign genetic background. Over the last 10 years, various protocols have thus been developed to apply this powerful technology to grapevine. Using cell suspension cultures, somatic embryos, leaves or whole plantlets, transient expression assays enabled the study of the function, regulation and subcellular localization of genes involved in specific metabolic pathways such as the biosynthesis of phenylpropanoids. Disease resistance genes that could be used for marker-assisted selection in conventional breeding or for stable transformation of elite cultivars have also been characterized. Additionally, transient expression assays have proved useful for shaping new tools for grapevine genetic improvement: synthetic promoters, silencing constructs, minimal linear cassettes or viral vectors. This review provides an update on the different tools (DNA constructs, reporter genes, vectors) and methods (Agrobacteriummediated and direct gene transfer methods) available for transient gene expression in grapevine. The most representative results published thus far are then described.

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Characterization of a putative grapevine Zn transporter, VvZIP3, suggests its involvement in early reproductive development in Vitis vinifera L

Cited by 47 publications

References 54 publications

Reference genomes and transcriptomes of Nicotiana sylvestris and Nicotiana tomentosiformis

Reference genomes and transcriptomes of Nicotiana sylvestris and Nicotiana tomentosiformis

Identification and characterization of the zinc-regulated transporters, iron-regulated transporter-like protein (ZIP) gene family in maize

Transient expression assays in grapevine: a step towards genetic improvement

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