Characterization of a potato leafroll luteovirus subgenomic RNA: differential expression by internal translation initiation and UAG suppression

The location of the 5' end of the potato leafroll luteovirus subgenomic coat protein mRNA

“…1990). However, this estimate is 172 nucleotides longer than that obtained for the German isolate of PLRV by Tacke et al (1990Tacke et al ( ) (i.e. 2334 nucleotides with the 5' terminus at nucleotide 3548).…”

“…This suggested a more precise location for the 5' end of the subgenomic RNA. Probe C extends from position 3365 to 3538, so its 3' end is 51 bases upstream of the start of the coat protein gene [and 11 bases upstream of the position proposed by Tacke et al (1990) for the 5' end of the subgenomic RNA]. These results suggest that the 5' end of the subgenomic RNA is between nucleotides 3370 and 3500.…”

The Potato Leafroll Luteovirus 17K Protein is a Single-stranded Nucleic Acid-binding Protein

“…By virtue of its ssRNA-binding capacity in the C-terminal domain and a putative dimerization domain at the N terminus, prl7 might protect PLRV RNA from degradation by cellular RNases and/or sequester genomic RNA to serve as a template in the replication complex. Such multiple functions may provide a clue as to why prl7 is translated from the same subgenomic RNA as CP but at a sevenfold higher efficiency (Tacke et al, 1990).…”

“…In PLRV-infected plants we have identified a 2-3 kb subgenomic RNA (sgRNA 1) which represents the 3' half of the viral genome (Tacke et al, 1990). This RNA is not encapsidated into viral particles (Smith & Harris, 1990) and contains the gene for the coat protein (CP, Tacke et al, 1989), which is separated by an amber stop codon from the ORF for a 56K protein (Fig.…”

Nucleotide sequence analysis of the genomes of the MAV-PS1 and P-PAV isolates of barley yellow dwarf virus

“…In fact, the transcriptional start site of a 2.6 kb subgenomic RNA from PLRV has recently been mapped to 34 nucleotides 5' of the coat protein translation initiation codon (Tacke et al, 1990) and corresponds to a conserved region identified in all other published luteovirus sequences (Vincent et al, 1991). Between MAV-PS1 and P-PAV there is 91~ sequence identity for the coat protein leader sequences; for P-PAV and Vic-PAV this region has 99~ identity.…”