Characterization of a pollen-specific cDNA clone from Nicotiana tabacum expressed during microgametogenesis and germination

Weterings, Koen; Reijnen, W. H.; R, van Aarssen; Kortstee, A.J.; Spijkers, J.; M, van Herpen; Schrauwen, J. A. M.; Wullems, G. J.

doi:10.1007/bf00047713

Cited by 74 publications

(64 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, their specific character was demonstrated by in vitro translation that presented EPPs as functional units with regulated translational activity. 33 The existence of these complexes explained the apparent discrepancy between the limitation of transcription to the first 3 h of tobacco pollen germination 29 and formation of 4 cm-long pollen tubes demanding massive translation. 48 Nevertheless, exact timing of derepression of mRNAs stored in EPP complexes remained unknown.…”

Section: Discussionmentioning

confidence: 99%

Cytoskeleton-Associated Large RNP Complexes in Tobacco Male Gametophyte (EPPs) Are Associated with Ribosomes and Are Involved in Protein Synthesis, Processing, and Localization

et al. 2009

View full text Add to dashboard Cite

The progamic phase of male gametophyte development involves activation of synthetic and catabolic processes required for the rapid growth of the pollen tube. It is well-established that both transcription and translation play an important role in global and specific gene expression patterns during pollen maturation. On the contrary, germination of many pollen species has been shown to be largely independent of transcription but vitally dependent on translation of stored mRNAs. Here, we report the first structural and proteomic data about large ribonucleoprotein particles (EPPs) in tobacco male gametophyte. These complexes are formed in immature pollen where they contain translationally silent mRNAs. Although massively activated at the early progamic phase, they also serve as a long-term storage of mRNA transported along with the translational machinery to the tip region. Moreover, EPPs were shown to contain ribosomal subunits, rRNAs and a set of mRNAs. Presented results extend our view of EPP complexes from mere RNA storage and transport compartment in particular stages of pollen development to the complex and well-organized machinery devoted to mRNA storage, transport and subsequent controlled activation resulting in protein synthesis, processing and precise localization. Such an organization is extremely useful in fast tip-growing pollen tube. There, massive and orchestrated protein synthesis, processing, and transport must take place in accurately localized regions. Moreover, presented complex role of EPPs in tobacco cytoplasmic mRNA and protein metabolism makes them likely to be active in another plant species too. Expression of vast majority of the closest orthologues of EPP proteins also in Arabidopsis male gametophyte further extends this concept from tobacco to Arabidopsis, the model species with advanced tricellular pollen.

show abstract

Section: Discussionmentioning

confidence: 99%

Cytoskeleton-Associated Large RNP Complexes in Tobacco Male Gametophyte (EPPs) Are Associated with Ribosomes and Are Involved in Protein Synthesis, Processing, and Localization

et al. 2009

View full text Add to dashboard Cite

show abstract

“…Moreover, the element AAATGA is repeated four times in the COPT1 promoter at positions Ϫ89, Ϫ452, Ϫ810, and Ϫ886. This element was identified in the promoter of the tobacco NTP303 and Arabidopsis AVP1 genes specifically expressed in pollen (38,39).…”

Section: Discussionmentioning

confidence: 99%

The Arabidopsis Copper Transporter COPT1 Functions in Root Elongation and Pollen Development

Sancenón

Puig

Mateu‐Andrés

et al. 2004

Journal of Biological Chemistry

247

197

View full text Add to dashboard Cite

Copper plays a dual role in aerobic organisms, as both an essential and a potentially toxic element. To ensure copper availability while avoiding its toxic effects, organisms have developed complex homeostatic networks to control copper uptake, distribution, and utilization. In eukaryotes, including yeasts and mammals, high affinity copper uptake is mediated by the Ctr family of copper transporters. This work is the first report on the physiological function of copper transport in Arabidopsis thaliana. We have studied the expression pattern of COPT1 in transgenic plants expressing a reporter gene under the control of the COPT1 promoter. The reporter gene is highly expressed in embryos, trichomes, stomata, pollen, and root tips. The involvement of COPT1 in copper acquisition was investigated in CaMV35S::COPT1 antisense transgenic plants. Consistent with a decrease in COPT1 expression and the associated copper deprivation, these plants exhibit increased mRNA levels of genes that are down-regulated by copper, decreased rates of 64 Cu uptake by seedlings and reduced steady state levels of copper as measured by atomic absorption spectroscopy in mature leaves. Interestingly, COPT1 antisense plants also display dramatically increased root length, which is completely and specifically reversed by copper addition, and an increased sensitivity to growth inhibition by the copper-specific chelator bathocuproine disulfonic acid. Furthermore, COPT1 antisense plants exhibit pollen development defects that are specifically reversed by copper. Taken together, these studies reveal striking plant growth and development roles for copper acquisition by high affinity copper transporters.

show abstract

“…Genes which are transiently and specifically transcribed during pollen development are assumed to play a crucial role in the developmental processes. Summarizing, genes involved in pollen development can be classified, according to expression pattern as early [dO, 28,35], and late genes [4,7,21,32,39,42]. Additionally, genes transcribed in both stages have been isolated [ 1,24,33].…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of a microspore-specific gene from tobacco

et al. 1996

View full text Add to dashboard Cite

The characterization of a gene with a unique microspore-specific expression pattern is reported. Isolated microspores from tobacco were used to synthesize a cDNA library. Clones that did not hybridize to leaf cDNA were further characterized by northern analysis. One clone proved to be a microspore-specific cDNA, representing a transcript of 650 nt. The corresponding gene, NTM19 (Nicotiana tabacum microspore-specific), was isolated and its sequence analysed. The gene encodes a protein of 10.8 kDa with a pI of 6.92 and a putative signal sequence at the N-terminus. A localization study revealed a unique spatial and temporal distribution. The transcript was only detected in the unicellular microspore. No hybridization signals were observed in other pollen developmental stages, nor in the surrounding anther tissues or other vegetative tissues of the plant. Therefore it can be concluded that NTM 19 is a gene with a highly microspore-specific character according to both localization and stage of expression. Southern blot analysis demonstrated the presence of a small gene family. The occurrence of TNM 19 was investigated in a range of closely and distantly related species and was found to be present in other solanaceous species, including the ancestors of tobacco and in a monocot species.

show abstract

Characterization of a pollen-specific cDNA clone from Nicotiana tabacum expressed during microgametogenesis and germination

Cited by 74 publications

References 42 publications

Cytoskeleton-Associated Large RNP Complexes in Tobacco Male Gametophyte (EPPs) Are Associated with Ribosomes and Are Involved in Protein Synthesis, Processing, and Localization

Cytoskeleton-Associated Large RNP Complexes in Tobacco Male Gametophyte (EPPs) Are Associated with Ribosomes and Are Involved in Protein Synthesis, Processing, and Localization

The Arabidopsis Copper Transporter COPT1 Functions in Root Elongation and Pollen Development

Isolation and characterization of a microspore-specific gene from tobacco

Contact Info

Product

Resources

About