Characterization of a phylogenetically distinct extradiol dioxygenase involved in the bacterial catabolism of lignin-derived aromatic compounds

Navas, Laura Emilce; Zahn, Michael; Bajwa, Harbir; Grigg, J.C.; Wolf, Megan E; Chan, Anson; Murphy, M.E.P.; McGeehan, J.E.; Eltis, Lindsay D.

doi:10.1016/j.jbc.2022.101871

Cited by 7 publications

(2 citation statements)

References 37 publications

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“…This was supported by similar abundances in the Lake Reference samples, which were not enclosed, nor subjected to any hydrocarbon additions during the monitoring period. The source of these mechanisms is likely from the degradation of complex and ubiquitous aromatics-based compounds, namely lignin, humic acids, and other complex organic molecules derived from woody and herbaceous plant matter in the organic-rich shoreline (De Haan 1976 , Navas et al 2022 ). These complex plant-based organic molecules are substrates for catechol 2,3 and catechol 1,2 dioxygenases, and their potential activity would be expected in an organic rich wetland shore type (De Haan 1976 , Briganti et al 1997 , Szabó et al 2007 ).…”

Section: Discussionmentioning

confidence: 99%

Influence of heavy Canadian crude oil on pristine freshwater boreal lake ecosystems in an experimental oil spill

Kharey,

Palace,

Whyte

et al. 2024

FEMS Microbiology Ecology

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The overall impact of a crude oil spill into a pristine freshwater environment in Canada is largely unknown. To evaluate the impact on the native microbial community, a large-scale in situ model experimental spill was conducted to assess the potential role of the natural community to attenuate hydrocarbons. A small volume of conventional heavy crude oil (CHV) was introduced within contained mesocosm enclosures deployed on the shoreline of a freshwater lake. The oil was left to interact with the shoreline for 72 h and then free-floating oil was recovered using common oil spill response methods (i.e. freshwater flushing and capture on oleophilic absorptive media). Residual PAH concentrations returned to near pre-oiling concentrations within 2 months, while the microbial community composition across the water, soil, and sediment matrices of the enclosed oligotrophic freshwater ecosystems did not shift significantly over this period. Metagenomic analysis revealed key polycyclic aromatic and alkane degradation mechanisms also did not change in their relative abundance over the monitoring period. These trends suggest that for small spills (<2 L of oil per 15 m2 of surface freshwater), physical oil recovery reduces PAH concentrations to levels tolerated by the native microbial community. Additionally, the native microbial community present in the monitored pristine freshwater ecosystem possesses the appropriate hydrocarbon degradation mechanisms without prior challenge by hydrocarbon substrates. This study corroborated trends found previously (Kharey et al. 2024) toward freshwater hydrocarbon degradation in an environmentally relevant scale and conditions on the tolerance of residual hydrocarbons in situ.

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Section: Discussionmentioning

confidence: 99%

Influence of heavy Canadian crude oil on pristine freshwater boreal lake ecosystems in an experimental oil spill

Kharey,

Palace,

Whyte

et al. 2024

FEMS Microbiology Ecology

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“…Sphingomonas paucimobilis SYK-6 cracked the benzene ring of MGA through DesZ and LigAB, and the hydroxyl group was oxidized to carbonyl group, resulting in 4-carboxyl-2-hydroxy-6-methoxy-6-oxyhexa-2, 4-dioleate (CHMOD) [ 105 ]. In Pseudomonas sp.CF600, the meta-cleavage pathway of catechol was found, and catechol was transformed by catechol 2,3 dioxygenase (C23O) to produce 2-hydroxyl mucosal hemialdehyde [ 106 ]. In addition, a study found that in S. paucimobilis SYK-6, with a biphenyl compound 2,2′-dihydroxy-3,3′-dimethoxy-5,5′-dicarboxylic biphenyl (DDVA) as the substrate, four enzymes with different functions were involved in the initial steps to degrade biphenyls.…”

Section: Lignin-degrading Enzymementioning

confidence: 99%

Bacterial transformation of lignin: key enzymes and high-value products

Gu,

Qiu,

et al. 2024

Biotechnol Biofuels

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Lignin, a natural organic polymer that is recyclable and inexpensive, serves as one of the most abundant green resources in nature. With the increasing consumption of fossil fuels and the deterioration of the environment, the development and utilization of renewable resources have attracted considerable attention. Therefore, the effective and comprehensive utilization of lignin has become an important global research topic, with the goal of environmental protection and economic development. This review focused on the bacteria and enzymes that can bio-transform lignin, focusing on the main ways that lignin can be utilized to produce high-value chemical products. Bacillus has demonstrated the most prominent effect on lignin degradation, with 89% lignin degradation by Bacillus cereus. Furthermore, several bacterial enzymes were discussed that can act on lignin, with the main enzymes consisting of dye-decolorizing peroxidases and laccase. Finally, low-molecular-weight lignin compounds were converted into value-added products through specific reaction pathways. These bacteria and enzymes may become potential candidates for efficient lignin degradation in the future, providing a method for lignin high-value conversion. In addition, the bacterial metabolic pathways convert lignin-derived aromatics into intermediates through the “biological funnel”, achieving the biosynthesis of value-added products. The utilization of this “biological funnel” of aromatic compounds may address the heterogeneous issue of the aromatic products obtained via lignin depolymerization. This may also simplify the separation of downstream target products and provide avenues for the commercial application of lignin conversion into high-value products.

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Perspective on Lignin Conversion Strategies That Enable Next Generation Biorefineries

Shrestha,

Goswami,

Banerjee

et al. 2024

ChemSusChem

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The valorization of lignin, a currently underutilized component of lignocellulosic biomass, has attracted attention to promote a stable and circular bioeconomy. Successful approaches including thermochemical, biological, and catalytic lignin depolymerization have been demonstrated, enabling opportunities for lignino‐refineries and lignocellulosic biorefineries. Although significant progress in lignin valorization has been made, this review describes unexplored opportunities in chemical and biological routes for lignin depolymerization and thereby contributes to economically and environmentally sustainable lignin‐utilizing biorefineries. This review also highlights the integration of chemical and biological lignin depolymerization and identifies research gaps while also recommending future directions for scaling processes to establish a lignino‐chemical industry.

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Characterization of a phylogenetically distinct extradiol dioxygenase involved in the bacterial catabolism of lignin-derived aromatic compounds

Cited by 7 publications

References 37 publications

Influence of heavy Canadian crude oil on pristine freshwater boreal lake ecosystems in an experimental oil spill

Influence of heavy Canadian crude oil on pristine freshwater boreal lake ecosystems in an experimental oil spill

Bacterial transformation of lignin: key enzymes and high-value products

Perspective on Lignin Conversion Strategies That Enable Next Generation Biorefineries

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