Characterization of a Pestivirus H isolate originating from goats

Abstract

Natural Pestivirus H infections in cattle have been reported worldwide; however, only a few cases of Pestivirus H have been described in non-bovine ruminants such as goats. A new Pestivirus H HN1507 strain was isolated from an infected goat in 2015 and the genome sequence was determined. The full-length genome sequence was 12,556 nucleotides. Phylogenetic analysis, based on the complete genome and Npro fragments, revealed that the isolate belonged to Pestivirus H and was closely related to strains from Italy. Two unique amino acid substitutions were found in the C-terminal of the E2 protein. To the best of our knowledge, this is first report determining the complete genome of a Pestivirus H strain from goat.

Genetic Diversity of Bovine Pestiviruses Detected in Backyard Cattle Farms Between 2014 and 2019 in Henan Province, China

“…Five isolates of the Pestivirus H "a" genotype were detected in Fangcheng and Shangcai in this study (Figures 1, 2). Before this research, Pestivirus H has been previously detected in goats and sheep in Fangcheng (24), and phylogenetic analysis showed that these five isolates were closely related to the HN1507 strain isolated from goat (43). These results indicated that these isolates shared the same origin.…”

“…BLAST analysis of the 5 ′ -UTR and Npro sequences showed that all five Pestivirus H-positive isolates belonged to Pestivirus H. Comparative analysis of the 5 ′ -UTR and Npro region of the Henan isolates and the reference strains of Pestivirus H (TH/04_KhonKaen, HN1507) revealed a sequence identity within the range of 87.5-99.5% and 90.3.2-99.4%, respectively. In particular, the nucleotide homologies between these isolates and the other strain (HN1507) (24,43) isolated from goat in the same area were 97.9±1.6% and 98.6 ± 0.4% in the above two regions.…”

“…For phylogenetic analysis, the BVDV-positive samples were further subjected to 5 ′ -UTR and Npro RT-PCR using primers 324/326 (41) and BD1/BD2 (42), respectively. Because the sequences of Pestivirus H-positive samples were most closely related to the HN1507 strain (43), the positive samples further subjected to RT-PCR covering a partial 5 ′ -UTR fragment and the entire Npro region with the primers HN-F (sense; 5 ′ -CCTTCAGTAGGACGAGCATAA-3 ′ ) and HN-R (antisense; 5 ′ -AGACGGGCTATACCACAATAA-3 ′ ), corresponding to nt 109-1,107 of Pestivirus H strain HN1507 (GenBank accession number: KU563155).…”