Characterization of a Pathogen Related to <i>Vavraia culicis</i> Detected in a Laboratory Colony of <i>Anopheles stephensi</i>

Lobo, Maria; Silveira, Henrique; Ramos, Susana; Xiao, Lihua; Matos, Olga

doi:10.1111/j.1550-7408.2006.00175.x

Cited by 6 publications

(5 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is most probable that future research will show that microsporidia recognized as Vavraia culicis-like are actually a group of closely related mosquito microsporidia of low host specificity and more or less adapted to individual hosts. The small, yet significant difference in the 16S rRNA gene sequence of the Vavraia culicis-like microsporidium (isolate AS) vis-à-vis the FL isolate (99% identity) (Lobo et al 2006) seems to support such a possibility.…”

Section: Envoi the Identity Of The Florida Isolatementioning

confidence: 84%

“…Microsporidia believed to be Vavraia culicis have been reported from mosquito hosts including at least five genera and more than 13 species collected in nature in a broad geographic area including Europe, Africa and North America (Weiser 1946, Weiser and Coluzzi 1964, 1972, Reynolds 1966, 1971, Clark and Fukuda 1971, Chapman 1974, Hazard and Chapman 1977, Fukuda et al 1997, Diarra and Toguebaye 1991. The organism was also found as a contaminant in laboratory mosquito colonies (Canning 1957, Lobo et al 2006 (Table 1). The low host-specificity and the ability to infect non-mosquito hosts (Weiser 1978, Kelly et al 1981, Becnel et al 2005) made microsporidia designated as Vavraia culicis a laboratory tool (Reynolds 1970, Agnew et al 1999, Cheney at al.…”

mentioning

confidence: 99%

“…This Florida (FL) isolate represents the only culture of a species of Vavraia isolated from mosquitoes that is maintained and grown under standard conditions. As it is available to other scientists, it has been used under the name Vavraia culicis in molecular phylogeny (Cheney et al 2000, 2001, Vávra et al 2006, host-parasite relationship studies (Agnew et al 1999, Becnel et al 2005, Bedhomme et al 2005, Biron et al 2005) and as comparative material in microsporidia identification (Lobo et al 2006). The present paper describes the structural characters of the FL isolate and discusses its taxonomic status.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Vavraia culicis (Weiser, 1947) Weiser, 1977 revisited: cytological characterisation of a Vavraia culicis-like microsporidium isolated from mosquitoes in Florida and the establishment of Vavraia culicis floridensis subsp. n.

Vávra¹,

Becnel²

2007

FOLIA PARASIT

View full text Add to dashboard Cite

Abstract. A brief nomenclatural history of Vavraia culicis (Weiser, 1947), the type species for the genus Vavraia Weiser, 1977, is presented together with a detailed description of the cytological and ultrastructural characteristics of a Vavraia culicis-like microsporidian species isolated from Aedes albopictus (Scuse) in Florida. This "Florida isolate", is the only known isolate of a species of the genus Vavraia from mosquitoes propagated in laboratory culture. Although the Florida isolate has been used under the name Vavraia culicis in several molecular phylogeny and host-parasite studies, it has not been structurally characterized and its relationship to the type species Vavraia culicis has never been examined. Structural data strongly support placement of the Florida isolate within the genus Vavraia and indicate its close relationship to both the type species of the genus and to other Vavraia-like mosquito microsporidia to which the name V. culicis has been applied. However, the identity of the Florida isolate with V. culicis (Weiser, 1947) Weiser, 1977 cannot be presently confirmed. Morphometric examination of spores of several Vavraia-like microsporidia isolates from mosquitoes, including the type material of Vavraia culicis, indicates that Vavraia culicis-like microsporidia probably represent not a single species, but a group of closely related organisms. Subspecies status is proposed for the Florida isolate.

show abstract

Section: Envoi the Identity Of The Florida Isolatementioning

confidence: 84%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Vavraia culicis (Weiser, 1947) Weiser, 1977 revisited: cytological characterisation of a Vavraia culicis-like microsporidium isolated from mosquitoes in Florida and the establishment of Vavraia culicis floridensis subsp. n.

Vávra¹,

Becnel²

2007

FOLIA PARASIT

View full text Add to dashboard Cite

show abstract

“…PCR detection for microsporidia known to infect A . stephensi was performed on the DNA extracts using three primer sets ( Nosema group, Pleistophora group and Brachiola algerae ) [26]. PCR products were extracted from gels, TA cloned in E .…”

Section: Methodsmentioning

confidence: 99%

Safety and Reproducibility of a Clinical Trial System Using Induced Blood Stage Plasmodium vivax Infection and Its Potential as a Model to Evaluate Malaria Transmission

et al. 2016

View full text Add to dashboard Cite

BackgroundInterventions to interrupt transmission of malaria from humans to mosquitoes represent an appealing approach to assist malaria elimination. A limitation has been the lack of systems to test the efficacy of such interventions before proceeding to efficacy trials in the field. We have previously demonstrated the feasibility of induced blood stage malaria (IBSM) infection with Plasmodium vivax. In this study, we report further validation of the IBSM model, and its evaluation for assessment of transmission of P. vivax to Anopheles stephensi mosquitoes.MethodsSix healthy subjects (three cohorts, n = 2 per cohort) were infected with P. vivax by inoculation with parasitized erythrocytes. Parasite growth was monitored by quantitative PCR, and gametocytemia by quantitative reverse transcriptase PCR (qRT-PCR) for the mRNA pvs25. Parasite multiplication rate (PMR) and size of inoculum were calculated by linear regression. Mosquito transmission studies were undertaken by direct and membrane feeding assays over 3 days prior to commencement of antimalarial treatment, and midguts of blood fed mosquitoes dissected and checked for presence of oocysts after 7–9 days.ResultsThe clinical course and parasitemia were consistent across cohorts, with all subjects developing mild to moderate symptoms of malaria. No serious adverse events were reported. Asymptomatic elevated liver function tests were detected in four of six subjects; these resolved without treatment. Direct feeding of mosquitoes was well tolerated. The estimated PMR was 9.9 fold per cycle. Low prevalence of mosquito infection was observed (1.8%; n = 32/1801) from both direct (4.5%; n = 20/411) and membrane (0.9%; n = 12/1360) feeds.ConclusionThe P. vivax IBSM model proved safe and reliable. The clinical course and PMR were reproducible when compared with the previous study using this model. The IBSM model presented in this report shows promise as a system to test transmission-blocking interventions. Further work is required to validate transmission and increase its prevalence.Trial RegistrationAnzctr.org.au ACTRN12613001008718

show abstract

“…There is emerging data that suggests that insects harboring microsporidian infections may serve as sources of zoonoses. A microsporidium infecting a laboratory colony of the mosquito Anopheles stephensi was found to have 99% and 97% 16S rRNA sequence identity to Vavraia culicis and B. algerae , respectively (Lobo et al). Based on molecular data, the former is closely related to the human pathogen Trachipleistophora hominis , while the latter is a human pathogen.…”

mentioning

confidence: 99%

Microsporidia 2006: IWOP‐9

Ghosh

Weiss

2006

J Eukaryotic Microbiology

View full text Add to dashboard Cite

Characterization of a Pathogen Related to Vavraia culicis Detected in a Laboratory Colony of Anopheles stephensi

Cited by 6 publications

References 6 publications

Vavraia culicis (Weiser, 1947) Weiser, 1977 revisited: cytological characterisation of a Vavraia culicis-like microsporidium isolated from mosquitoes in Florida and the establishment of Vavraia culicis floridensis subsp. n.

Vavraia culicis (Weiser, 1947) Weiser, 1977 revisited: cytological characterisation of a Vavraia culicis-like microsporidium isolated from mosquitoes in Florida and the establishment of Vavraia culicis floridensis subsp. n.

Safety and Reproducibility of a Clinical Trial System Using Induced Blood Stage Plasmodium vivax Infection and Its Potential as a Model to Evaluate Malaria Transmission

Microsporidia 2006: IWOP‐9

Contact Info

Product

Resources

About