Characterization of a novel β-barrel protein (AtOM47) from the mitochondrial outer membrane of<i>Arabidopsis thaliana</i>

Li, Lü; Kubiszewski-Jakubiak, Szymon; Radomiljac, Jordan D.; Wang, Yan; Law, Simon R.; Keech, Olivier; Narsai, Reena; Berkowitz, Oliver; Duncan, Owen; Murcha, Monika W.; Whelan, James

doi:10.1093/jxb/erw366

Cited by 16 publications

(11 citation statements)

References 63 publications

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“…Arabidopsis thaliana ecotype Columbia‐0 (Col‐0) was used as the wild‐type control for all experiments unless stated otherwise. aox1a (Giraud et al ., ); At12cys‐1 , At12cys‐2a , At12cys‐2b , ndufs4 , rug3 , tim23‐2 KO , TIM23‐2 OE (Wang et al ., ); kin10 , rao1‐2 , CDKE1‐OE (Ng et al ., ); myb29‐1 , myb29‐2 (Zhang et al ., ); mia40 (Carrie et al ., ); om47‐1 , om47‐2 (Li et al ., ); om66‐1 , OM66 OE (Zhang et al ., ); rao2‐1 , ANAC017‐OE (Ng et al ., ); rao3 (Ivanova et al ., ); WRKY15 OE , wrky33‐1 , wrky33‐2 , WRKY40 OE , wrky40‐1 , wrky42‐1 (Van Aken et al ., ); abi4‐1 (Giraud et al ., ); axr4 (Hobbie and Estelle, ); coi1‐16 (Ellis and Turner, ); eds5‐1 (Glazebrook et al ., ); ein2‐1 , etr1‐3 (Guzman and Ecker, ); gai‐1 (Koorneef et al ., ); gai‐t6/rga‐t2/rgl1‐1/rgl2‐1/rgl3‐1 (Feng et al ., ); yucca (Zhao et al ., ). The coding sequence of At12CYS‐1 (At5 g64400) and At12CYS‐2 (At5 g09570) was cloned to pk7WG2 using the Gateway methods to obtain the At12CYS‐1‐OE and At12CYS‐2‐OE overexpression constructs, respectively.…”

Section: Methodsmentioning

confidence: 99%

Mitochondrial function modulates touch signalling in Arabidopsis thaliana

Berkowitz

Narsai

et al. 2019

The Plant Journal

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Plants respond to short-and long-term mechanical stimuli, via altered transcript abundance and growth respectively. Jasmonate, gibberellic acid and calcium have been implicated in mediating responses to mechanical stimuli. Previously it has been shown that the transcript abundance for the outer mitochondrial membrane protein of 66 kDa (OM66), is induced several fold after 30 min in response to touch. Therefore, the effect of mitochondrial function on the response to mechanical stimulation by touch at 30 min was investigated. Twenty-five mutants targeting mitochondrial function or regulators revealed that all affected the touch transcriptome. Double and triple mutants revealed synergistic or antagonistic effects following the observed responses in the single mutants. Changes in the touch-responsive transcriptome were localised, recurring with repeated rounds of stimulus. The gene expression kinetics after repeated touch were complex, displaying five distinct patterns. These transcriptomic responses were altered by some regulators of mitochondrial retrograde signalling, such as cyclic dependent protein kinase E1, a kinase protein in the mediator complex, and KIN10 (SnRK1 À sucrose non-fermenting related protein kinase 1), revealing an overlap between the touch response and mitochondrial stress signalling and alternative mitochondrial metabolic pathways. Regulatory network analyses revealed touch-induced stress responses and suppressed growth and biosynthetic processes. Interaction with the phytohormone signalling pathways indicated that ethylene and gibberellic acid had the greatest effect. Hormone measurements revealed that mutations of genes that encoded mitochondrial proteins altered hormone concentrations. Mitochondrial function modulates touch-induced changes in gene expression directly through altered regulatory networks, and indirectly via altering hormonal levels.

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Section: Methodsmentioning

confidence: 99%

Mitochondrial function modulates touch signalling in Arabidopsis thaliana

Berkowitz

Narsai

et al. 2019

The Plant Journal

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“…Notably, the dgs1-2 null mutant did not affect the abundance of any protein or the activity of the respiratory chain ( Figures 5A and 5B). However, both the dgs1-1 mutant and the dgs1-1 Comp (L) line with protein levels comparable to the wild type showed decreased abundance of an outer membrane (OM) b-barrel protein in plant mitochondria (OM47; Li et al, 2016b), TIM44, and AOX ( Figure 5A). The consistent decrease in abundance of TOM20s ( Figure 2B), OM47, TIM44, and AOX ( Figure 5A) in all lines expressing the dgs1-1 mutant protein indicates that the mutated dgs1-1 protein has an effect on specific proteins or pathways.…”

Section: The Dgs1-1 Mutation Alters Mitochondrial Lipid Compositionmentioning

confidence: 99%

“…The total chlorophyll content was measured as described previously (Li et al, 2016b). The chlorophyll fluorescence F v /F m (maximum quantum yield of PSII) was pulsed with 120 mmol m 22 s 21 of actinic light using the IMAGING-PAM M-series Chlorophyll Fluorescence System (Walz) after a 20 min of dark acclimation as described previously (Rossel et al, 2006).…”

Section: Chlorophyll Analysis and Fluorescencementioning

confidence: 99%

Arabidopsis DGD1 SUPPRESSOR1 Is a Subunit of the Mitochondrial Contact Site and Cristae Organizing System and Affects Mitochondrial Biogenesis

Lavell

Meng

et al. 2019

The Plant Cell

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Mitochondrial and plastid biogenesis requires the biosynthesis and assembly of proteins, nucleic acids, and lipids. In Arabidopsis (Arabidopsis thaliana), the mitochondrial outer membrane protein DGD1 SUPPRESSOR1 (DGS1) is part of a large multi-subunit protein complex that contains the mitochondrial contact site and cristae organizing system 60-kD subunit, the translocase of outer mitochondrial membrane 40-kD subunit (TOM40), the TOM20s, and the Rieske FeS protein. A point mutation in DGS1, dgs1-1, altered the stability and protease accessibility of this complex. This altered mitochondrial biogenesis, mitochondrial size, lipid content and composition, protein import, and respiratory capacity. Whole plant physiology was affected in the dgs1-1 mutant as evidenced by tolerance to imposed drought stress and altered transcriptional responses of markers of mitochondrial retrograde signaling. Putative orthologs of Arabidopsis DGS1 are conserved in eukaryotes, including the Nuclear Control of ATP Synthase2 (NCA2) protein in yeast (Saccharomyces cerevisiae), but lost in Metazoa. The genes encoding DGS1 and NCA2 are part of a similar coexpression network including genes encoding proteins involved in mitochondrial fission, morphology, and lipid homeostasis. Thus, DGS1 links mitochondrial protein and lipid import with cellular lipid homeostasis and whole plant stress responses.

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“…AsA and DHA levels were determined as previously described [7]. A dark-induced senescence assay was performed where two-week-old Arabidopsis seedlings were subjected to continuous dark treatment for 3 d and 6 d and then light recovery for 16 h. Chlorophyll content determination in Arabidopsis seedlings were conducted as described elsewhere [31,72]. The expression levels of flowering-related genes such as GI, FVE, LFY, CO, FT, AP1, SOC1, and FLC; and SAGs, including PIFs (PIF3, PIF4, and PIF5), SENs (SEN1 and SEN4), SAG20, TFs (WRKY6, NAP, and NAC2), cell-programmed death genes (ACD1 and PDCD5), and PHENOPHYTIN PHEOPHORBIDE HYDROLASE (PPH), were determined by qRT-PCR using Arabidopsis thaliana 18S ribosomal RNA (At18SrRNA) and Arabidopsis thaliana Actin 2 (AtACTIN2) genes as reference genes to normalize the qRT-PCR data, with the gene-specific primers listed in Supplementary Table S4.…”

Section: Functional Analysis Of Ghao1a In Arabidopsismentioning

confidence: 99%

Genome-Wide Identification and Expression Analysis of the Ascorbate Oxidase Gene Family in Gossypium hirsutum Reveals the Critical Role of GhAO1A in Delaying Dark-Induced Leaf Senescence

Pan

Chen

Wang

et al. 2019

IJMS

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Ascorbate oxidase (AO) plays important roles in plant growth and development. Previously, we reported a cotton AO gene that acts as a positive factor in cell growth. Investigations on Gossypium hirsutum AO (GhAO) family genes and their multiple functions are limited. The present study identified eight GhAO family genes and performed bioinformatic analyses. Expression analyses of the tissue specificity and developmental feature of GhAOs displayed their diverse expression patterns. Interestingly, GhAO1A demonstrated the most rapid significant increase in expression after 1 h of light recovery from the dark. Additionally, the transgenic ao1-1/GhAO1A Arabidopsis lines overexpressing GhAO1A in the Arabidopsis ao1-1 late-flowering mutant displayed a recovery to the normal phenotype of wild-type plants. Moreover, compared to the ao1-1 mutant, the ao1-1/GhAO1A transgenic Arabidopsis presented delayed leaf senescence that was induced by the dark, indicating increased sensitivity to hydrogen peroxide (H2O2) under normal conditions that might be caused by a reduction in ascorbic acid (AsA) and ascorbic acid/dehydroascorbate (AsA/DHA) ratio. The results suggested that GhAOs are functionally diverse in plant development and play a critical role in light responsiveness. Our study serves as a foundation for understanding the AO gene family in cotton and elucidating the regulatory mechanism of GhAO1A in delaying dark-induced leaf senescence.

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Characterization of a novel β-barrel protein (AtOM47) from the mitochondrial outer membrane ofArabidopsis thaliana

Abstract: HighlightCharacterization of a novel β-barrel protein in Arabidopsis identifies its role in leaf senescence by mediating the transport of chloroplast breakdown products across the outer mitochondrial membrane for their recycling.

Cited by 16 publications

References 63 publications

Mitochondrial function modulates touch signalling in Arabidopsis thaliana

Mitochondrial function modulates touch signalling in Arabidopsis thaliana

Arabidopsis DGD1 SUPPRESSOR1 Is a Subunit of the Mitochondrial Contact Site and Cristae Organizing System and Affects Mitochondrial Biogenesis

Genome-Wide Identification and Expression Analysis of the Ascorbate Oxidase Gene Family in Gossypium hirsutum Reveals the Critical Role of GhAO1A in Delaying Dark-Induced Leaf Senescence

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