Characterization of a novel xylanase from an extreme temperature hot spring metagenome for xylooligosaccharide production

Joshi, Namrata; Sharma, Manisha; Singh, Sudhir P.

doi:10.1007/s00253-020-10562-7

Cited by 38 publications

(19 citation statements)

References 61 publications

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“…Metagenomics can exert a significant impact on understanding the microbial niches of the extreme habitats, such as hot springs, glacial lakes, hydrothermal vents, etc., which are often found in isolated places. Further, the bioinformatic analysis of the assembled-metagenomic information generated from the extreme environmental samples, such as hot-springs, has led to the discovery of novel genes encoding desirable biocatalysts of societal importance [2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Metagenomics revealing molecular profiling of community structure and metabolic pathways in natural hot springs of the Sikkim Himalaya

Sharma

Kumar

Abedin³

et al. 2020

BMC Microbiol

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Background Himalaya is an ecologically pristine environment. The geo-tectonic activities have shaped various environmental niches with diverse microbial populations throughout the Himalayan biosphere region. Albeit, limited information is available in terms of molecular insights into the microbiome, including the uncultured microbes, of the Himalayan habitat. Hence, a vast majority of genomic resources are still under-explored from this region. Metagenome analysis has simplified the extensive in-depth exploration of diverse habitats. In the present study, the culture-independent whole metagenome sequencing methodology was employed for microbial diversity exploration and identification of genes involved in various metabolic pathways in two geothermal springs located at different altitudes in the Sikkim Himalaya. Results The two hot springs, Polok and Reshi, have distinct abiotic conditions. The average temperature of Polok and Reshi was recorded to be 62 °C and 43 °C, respectively. Both the aquatic habitats have alkaline geochemistry with pH in the range of 7–8. Community profile analysis revealed genomic evidence of plentiful bacteria, with a minute fraction of the archaeal population in hot water reservoirs of Polok and Reshi hot spring. Mesophilic microbes belonging to Proteobacteria and Firmicutes phyla were predominant at both the sites. Polok exhibited an extravagant representation of Chloroflexi, Deinococcus-Thermus, Aquificae, and Thermotogae. Metabolic potential analysis depicted orthologous genes associated with sulfur, nitrogen, and methane metabolism, contributed by the microflora in the hydrothermal system. The genomic information of many novel carbohydrate-transforming enzymes was deciphered in the metagenomic description. Further, the genomic capacity of antimicrobial biomolecules and antibiotic resistance were discerned. Conclusion The study provided comprehensive molecular information about the microbial treasury as well as the metabolic features of the two geothermal sites. The thermal aquatic niches were found a potential bioresource of biocatalyst systems for biomass-processing. Overall, this study provides the whole metagenome based insights into the taxonomic and functional profiles of Polok and Reshi hot springs of the Sikkim Himalaya. The study generated a wealth of genomic data that can be explored for the discovery and characterization of novel genes encoding proteins of industrial importance.

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Section: Introductionmentioning

confidence: 99%

Metagenomics revealing molecular profiling of community structure and metabolic pathways in natural hot springs of the Sikkim Himalaya

Sharma

Kumar

Abedin³

et al. 2020

BMC Microbiol

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“…B6 had a specific activity of 763.74 U/mg (Liu et al, 2020). However, to the best of our knowledge, there has been no previous report of xylanase that could possess a specific activity, as high as 1,671.37 ± 13.31 U/mg on beechwood xylan so far (Bala & Singh, 2019;Joshi et al, 2020;Long et al, 2018;Zhou et al, 2020).…”

Section: Enzyme Kineticsmentioning

confidence: 82%

A novel thermostable xylanase from Geobacillus vulcani GS90: Production, biochemical characterization, and its comparative application in fruit juice enrichment

2021

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Xylanases have great attention to act as a potential role in agro-industrial processes.In this study, production, characterization, and fruit juice application of novel xylanase from thermophilic Geobacillus vulcani GS90 (GvXyl) were performed. GvXyl was purified via acetone precipitation and gel-filtration chromatography. The results showed that GvXyl had 1,671.4 U/mg of specific activity and optimally worked at pH 8 and 55°C. It was also active in a wide pH (3-9) and temperature (30-90ºC) ranges.GvXyl was highly stable at 90ºC and relatively stable at pH 3-9. The kinetic parameters of GvXyl were obtained as K m , V max , and k cat ; 10.2 mg/ml, 4,104 µmol min −1 mg −1 , and 3,542.6 s −1 , respectively. GvXyl had higher action than commercial xylanase in fruit juice enrichment. These results revealed that GvXyl might possess a potential influence in fruit juice processing because of its high specific activity and great thermal stability.

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“…Several genetic engineering approaches have been used to improve the catalytic activity and stability of these enzymes. A recent study identified a thermostable xylanase gene from the metagenomic sequence data generated from a geothermal aquatic habitat of extreme temperature (98°C) (Joshi, Sharma, & Singh, 2020 (Basinskiene, Garmuviene, Juodeikiene, & Haltrich, 2007;Rosell & Dura, 2015). Xylanase from A. oryzae was found to be the best bread improver, while the one from Trichoderma reesei showed superior antistaling property (Rosell & Dura, 2015).…”

Section: Xylanasementioning

confidence: 99%

“…Several genetic engineering approaches have been used to improve the catalytic activity and stability of these enzymes. A recent study identified a thermostable xylanase gene from the metagenomic sequence data generated from a geothermal aquatic habitat of extreme temperature (98°C) (Joshi, Sharma, & Singh, 2020). The metagenomic DNA fragment was cloned and introduced in Escherichia coli cells for heterologous protein expression.…”

Section: Categories and Applications Of Food Enzymes From Extremophilesmentioning

confidence: 99%

“…The recombinant protein was characterized and found to be highly stable when exposed to a broad range of temperature, pH, surfactants, metal ions, and organic solvents. The novel xylanase was able to hydrolyze hemicellulosic polymer beechwood xylan, wheat arabinoxylan, corncob xylan, and sweet sorghum xylan (Joshi et al., 2020).…”

Section: Categories and Applications Of Food Enzymes From Extremophilesmentioning

confidence: 99%

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Revisiting the scope and applications of food enzymes from extremophiles

Akanbi

Agyei

2020

J. Food Biochem.

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Characterization of a novel xylanase from an extreme temperature hot spring metagenome for xylooligosaccharide production

Cited by 38 publications

References 61 publications

Metagenomics revealing molecular profiling of community structure and metabolic pathways in natural hot springs of the Sikkim Himalaya

Metagenomics revealing molecular profiling of community structure and metabolic pathways in natural hot springs of the Sikkim Himalaya

A novel thermostable xylanase from Geobacillus vulcani GS90: Production, biochemical characterization, and its comparative application in fruit juice enrichment

Revisiting the scope and applications of food enzymes from extremophiles

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