Characterization of a Novel Tumorigenic Esophageal Adenocarcinoma Cell Line: OANC1

Abstract

OANC1 represents a valuable addition to the limited range of pre-clinical models for EAC. This new cell line will be a useful model system for researchers studying both basic and translational aspects of this disease.

Authentication and characterisation of a new oesophageal adenocarcinoma cell line: MFD-1

“…This adds substantially to the utility of MFD-1 as a pre-clinical model of OAC and enables complex experiments that may include other constituents of the tumour microenvironment and/or manipulation of the host organism to mimic human treatments, such as novel CAF targeted agents or combination therapies. Similarly to the results reported by Clemons et al 14 we found that FLO1 and OE33 are poorly tumourigenic in SCID mice, this is somewhat surprising given that OE33 is one of the most commonly used OAC cell lines in xenograft experiments, however these findings reinforce the tumourigenic nature of MFD-1.…”

“…Similar problems exist for all historic OAC cell lines. More recently Clemons and colleagues developed the OANC1 cell line14. Authenticity was confirmed by STR comparing the cell line and tumour of origin at 16 loci and targeted deep sequencing of 48 cancer related genes was performed, but only in the cell line.…”

“…New, well-described and authenticated, cell lines derived from OAC without any in-vitro modification are valuable tools, such as the recently introduced OANC11415. But these cell lines may or may not be representative of the mutational profile of cells driving the malignancy in vivo 16.…”

APR-246 potently inhibits tumour growth and overcomes chemoresistance in preclinical models of oesophageal adenocarcinoma

“…FLO-1, OACM5.1, Eso26, SKGT4, OACP4C and TE7 cells were gifts from Professor Rebecca Fitzgerald (University of Cambridge, UK). OANC1 cells were derived in our laboratory 16. Immortalised oesophageal squamous epithelial cells (NES) were a gift from Professor Rhonda Souza (University of Texas Southwestern Medical Centre, Dallas, Texas, USA) 17.…”

Intramuscular Transplantation Improves Engraftment Rates for Esophageal Patient-Derived Tumor Xenografts

“…Targeted sequencing across a panel of 48 known oncogenes was then performed using the Illumina TruSeq Amplicon Cancer Panel as previously described. 16 Therapeutic Response…”