Characterization of a novel strain of Aspergillus aculeatinus: From rhamnogalacturonan type I pectin degradation to improvement of fruit juice filtration

Garzón, Catalina; Habrylo, Olivier; Lemaire, Adrien; Antalick, Guillaume; Carré, Yoann; Millet, Clémence; Fourtot-Brun, Catherine; Trezel, Pauline; Blond, Pascal Le; Perrin, Aurore; Georgé, Stéphane; Wagner, Magali; Coutel, Y; Levavasseur, Loïc; Pau-Roblot, Corinne; Pelloux, Jérôme

doi:10.1016/j.carbpol.2021.117943

Cited by 9 publications

(1 citation statement)

References 53 publications

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“…Aspergillus spp. has been proven to produce cellulase, hemicellulose and pectinase, and can be used to clarify fruit juice [6]. Xylanase can also improve the clarity of juice [7,8].…”

Section: Introductionmentioning

confidence: 99%

Safety Evaluation and Whole Genome Sequencing of Aspergillus japonicas PJ01 Reveal Its Potential to Degrade Citrus Segments in Juice Processing

Qian

Gao

Wang

et al. 2021

Foods

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Aspergillus japonicas PJ01 (A. japonicas PJ01) is a strain isolated from the rotten branches. In previ-ous studies, it was shown that it can produce complex enzymes to degrade polysaccharide com-ponents. In this study, we evaluated the safety of its crude enzyme solution. Acute oral toxicity, subchronic toxicity, micronucleus and sperm malformation tests all validated the high biologi-cal safety for the crude enzymes. Secondly, we carried out the citrus segment degradation ex-periment of crude enzyme solution. Compared with the control group, the crude enzyme solu-tion of A. japonicas PJ01 can completely degrade the segments in 50 min, which provides the basis for enzymatic peeling during juice processing. The whole genome sequencing showed that the genome of A. japonicus PJ01 has a GC content of 51.37% with a size of 36204647 bp, and encoded 10070 genes. GO, COG, KEGG and CAZy databases were used in gene annotation analyses. Pathway enrichment showed many genes related to carbohydrate metabolism, rich in genes re-lated to pectinase, xylanase and carboxylcellulase. Therefore, the complex enzyme produced by A. japonicus PJ01 can be used in gizzard juice processing to achieve efficient enzymatic decapsu-lation.

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“…Aspergillus spp. has been proven to produce cellulase, hemicellulose and pectinase, and can be used to clarify fruit juice [6]. Xylanase can also improve the clarity of juice [7,8].…”

Section: Introductionmentioning

confidence: 99%

Safety Evaluation and Whole Genome Sequencing of Aspergillus japonicas PJ01 Reveal Its Potential to Degrade Citrus Segments in Juice Processing

Qian

Gao

Wang

et al. 2021

Foods

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Space exposure enhanced pectin-degrading enzymes expression and activity in Aspergillus costaricaensis

Song,

Li,

et al. 2023

World J Microbiol Biotechnol

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Aspergillus is a well-studied fungal genus that is widely used in the processing of plant biomass in industries. This study investigated the effects of space exposure on the ability of Aspergillus costaricaensis, a lamentous fungus isolated from rotten orange peel, to degrade pectin. The fungus was carried into space by the Long March 5B carrier rocket and exposed to cosmic radiation for 79 hours.After the ight, the strains were screened using pectin as the sole carbon source for directed screening and pectinase activity evaluation. The space-exposed strains showed increased biomass accumulation ability and pectin-degrading activity compared to the ground control strain. Comparative transcriptome analysis revealed that several CAZymes genes were signi cantly upregulated in the space-exposed strains, especially those related to pectin degradation. Among the 44 pectinases identi ed from the annotated genome, 42 were up-regulated. The identi ed activities of these pectinases can break down the four basic structures of pectin, and work together to break down pectin substrates. In addition, the expression of some genes involved in metabolism, sugar transport, and stress response were altered. These results suggest that the space environment can be used as a mutagenesis breeding technique to create highly e cient biomass-degrading microbial strains, with potential applications in biotechnology and bioengineering.

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Production and Biochemical Characterization of Aspergillus japonicus Pectinase Using a Low-Cost Alternative Carbon Source for Application in the Clarification of Fruit Juices

de Alencar Guimarães,

Glienke,

Contato

et al. 2023

Waste Biomass Valor

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Characterization of a novel strain of Aspergillus aculeatinus: From rhamnogalacturonan type I pectin degradation to improvement of fruit juice filtration

Cited by 9 publications

References 53 publications

Safety Evaluation and Whole Genome Sequencing of Aspergillus japonicas PJ01 Reveal Its Potential to Degrade Citrus Segments in Juice Processing

Safety Evaluation and Whole Genome Sequencing of Aspergillus japonicas PJ01 Reveal Its Potential to Degrade Citrus Segments in Juice Processing

Space exposure enhanced pectin-degrading enzymes expression and activity in Aspergillus costaricaensis

Production and Biochemical Characterization of Aspergillus japonicus Pectinase Using a Low-Cost Alternative Carbon Source for Application in the Clarification of Fruit Juices

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