Characterization of a Novel IncHI2 Plasmid Carrying Tandem Copies of
 bla
 CTX-M-2
 in a
 fosA6
 -Harboring Escherichia coli Sequence Type 410 Strain

Guo, Qinglan; Ding, Baixing; Jové, Thomas; Stoesser, Nicole; Cooper, Vaughn S.; Wang, Minggui; Doi, Yohei

doi:10.1128/aac.01173-16

Cited by 12 publications

(6 citation statements)

References 35 publications

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“…ST2-, ST3-, and ST4-IncHI2 plasmids mostly clustered together, and most ST1-IncHI2 plasmids clustered separately. This phenomenon seemed to be consistent with phylogenetic analysis of replication regions from 24 fully sequenced IncHI2 plasmids (20). Clusters I and II can be further subdivided into seven (groups A1 to A7) and two (groups B1 and B2) subgroups, respectively.…”

supporting

confidence: 85%

High Genetic Plasticity in Multidrug-Resistant Sequence Type 3-IncHI2 Plasmids Revealed by Sequence Comparison and Phylogenetic Analysis

Fang

Deng

et al. 2018

Antimicrob Agents Chemother

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We report a novel fusion plasmid, pP2-3T, cointegrating sequence type 3 (ST3)-IncHI2 with an IncFII plasmid backbone mediating multidrug resistance (MDR) and virulence. Phylogenetic analysis and comparative genomics revealed that pP2-3T and other MDR ST3-IncHI2 plasmids clustered together, representing a unique IncHI2 lineage that exhibited high conservation in backbones of plasmids but possessed highly genetic plasticity in various regions by acquiring numerous antibiotic resistance genes and fusing with other plasmids. Surveillance studies should be performed to monitor multiresistance IncHI2 plasmids among .

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supporting

confidence: 85%

High Genetic Plasticity in Multidrug-Resistant Sequence Type 3-IncHI2 Plasmids Revealed by Sequence Comparison and Phylogenetic Analysis

Fang

Deng

et al. 2018

Antimicrob Agents Chemother

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“…Plasmids are important vehicles for the carriage of other MGEs and usually acquire ARGs associated with these elements ( Partridge et al, 2018 ). IncHI2 plasmids are very flexible and genetically plastic, and the highly active traits in acquiring antibiotic resistance determinants make them the primary vector responsible for ARGs transmission ( Garcia et al, 2007 ; Cain and Hall, 2012 ; Chen et al, 2016 ; Guo et al, 2016 ; Wong et al, 2016 ; Li et al, 2017b , 2020 ; Fang et al, 2018 ; Zhao et al, 2018 ; Wyrsch et al, 2019 ; Zingali et al, 2020 ). Cephalosporin resistance genes bla CTX–M have often been found on diverse transmissible plasmids ( Canton et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Formation, Transmission, and Dynamic Evolution of a Multidrug-Resistant Chromosomally Integrated Plasmid in Salmonella Spp.

Chang

Zhang

et al. 2022

Front. Microbiol.

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IncHI2 plasmids, possessing high flexibility and genetic plasticity, play a vital role in the acquisition and transmission of resistance determinants. Polymorphic mobile genetic elements (MGEs) generated by a chromosomally integrated IncHI2 plasmid in an individual Salmonella isolate have not yet been detected, and the mechanisms of the formation, excision, and dynamic evolution of a multidrug-resistant chromosomally integrated plasmid (MRCP) have remained obscure. Herein, we identified a 260-kb blaCTX–M–55-qnrS1-bearing IncHI2 plasmid within a Salmonella Muenster strain. Plenty of heterogeneous MGEs (new Escherichia coli chromosomally integrated plasmid or circular plasmids with different profiles) were yielded when this MRCP was conjugated into E. coli J53 with a transfer frequency of 10–4–10–5 transconjugants per donor. A bioinformatic analysis indicated that replicative transposition and homologous recombination of IS26 elements were particularly active, and the truncated Tn1721 also played a vital role in the formation of MRCP offspring. More importantly, when released from the chromosome, MRCP could capture and co-transfer adjacent chromosomal segments to form larger plasmid progeny than itself. Stability and growth kinetics assays showed that the biological characteristics of MRCP progeny were differentiated. This study provides an insight into a flexible existence of MRCP. The conversion between vertical and horizontal transmission endowed MRCP with genetic stability as a chromosomal coding structure and transferability as extra-chromosomal elements. This alternation may accelerate the acquisition and persistence of antibiotic resistance of clinical pathogens and enhance their ability to respond to adverse environments, which poses a great challenge to the traditional antibiotic treatment.

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“…coli YD786 carries four plasmids including fosA6-harboring pYD786-2 (GenBank accession no. KU254579.1), bla CTX-M-2 -harboring pYD786-1 (KU254578.1), and two IncX plasmids (pYD786-3 and pYD786-4), which were sequenced and assembled as reported before (12,20). BLAST tools (http://blast .ncbi.nlm.nih.gov/Blast.cgi) were used to search for homologues of IncX plasmid backbone.…”

Section: Methodsmentioning

confidence: 99%

IncX2 and IncX1-X2 Hybrid Plasmids Coexisting in a FosA6-Producing Escherichia coli Strain

Guo

McElheny

et al. 2017

Antimicrob Agents Chemother

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IncX plasmids are receiving much attention as vehicles of carbapenem and colistin resistance genes, such as bla NDM , bla KPC , and mcr-1. Among them, IncX2 subgroup plasmids remain rare. Here, we characterized IncX2 and IncX1-X2 hybrid plasmids coexisting in a FosA6-producing Escherichia coli strain that were possibly generated as a consequence of recombination events between an R6K-like IncX2 plasmid and a pLN126_33-like IncX1 plasmid. Variable multidrug resistance mosaic regions were observed in these plasmids, indicating their potential to serve as flexible carriers of resistance genes. The diversity of IncX group plasmid backbones and accessory genes and the evolution of hybrid IncX plasmids pose a challenge in detecting and classifying them.KEYWORDS IncX plasmid, taxC, pir, genotyping, glutathione synthetase T he IncX family plasmids are narrow-host-range plasmids mostly found in Enterobacteriaceae (1-3). IncX plasmids contain a highly syntenic backbone consisting of core genes responsible for plasmid replication, partitioning, maintenance, and conjugal DNA transfer (2). So far, at least eight subgroups of IncX plasmids have been reported, IncX1 to IncX8, carrying various resistance genes, including oqxAB, qnrS1, qnrS2, bla TEM , bla SHV , bla CTX-M , bla KPC , bla NDM , and mcr-1, and mediating resistance to fluoroquinolones, cephalosporins, carbapenems, colistin, or other types of antibiotics (2-9). Plasmid R6K, recovered from an Escherichia coli strain cultured in Greece in the 1960s, is considered to be the prototypical IncX plasmid (1). R6K belongs to a rare IncX subgroup, IncX2, with only two other currently recognized members, pNGX2-QnrS1 (GenBank accession number JQ269335.1) and pEBG1 (GenBank accession number KF738053.1), both discovered in strains from western Nigeria and carrying qnrS1 genes (3, 10). In contrast, the IncX1, IncX3, and IncX4 subgroup plasmids are much more prevalent and are associated with oqxAB, bla NDM , respectively (2,3,7,8,11). Here, we describe a new IncX2 plasmid, pYD786-4, that coexisted with a hybrid IncX1-X2 plasmid pYD786-3 in an E. coli ST410 urinary tract strain collected from a patient in the United States (12). RESULTS AND DISCUSSIONHybrid IncX1-X2 plasmid pYD786-3. Plasmid pYD786-3 is 44,806 bp in size and shares conserved backbone modules of both IncX1 and IncX2 plasmids. The replication, partitioning, and stability regions are from the IncX1 plasmid, while the maintenance and DNA transfer regions are from the IncX2 plasmid (Fig. 1). pir and bis encode the plasmid initiation replication protein and auxiliary protein that bind to the core region of replication gamma origin (2). parGF and stbED have putative functions of partitioning and stability. Two long inverted repeats (LIRs), ␣-LIR and ␤-LIR, contain origins of

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Characterization of a Novel IncHI2 Plasmid Carrying Tandem Copies of bla _CTX-M-2 in a fosA6 -Harboring Escherichia coli Sequence Type 410 Strain

Cited by 12 publications

References 35 publications

High Genetic Plasticity in Multidrug-Resistant Sequence Type 3-IncHI2 Plasmids Revealed by Sequence Comparison and Phylogenetic Analysis

High Genetic Plasticity in Multidrug-Resistant Sequence Type 3-IncHI2 Plasmids Revealed by Sequence Comparison and Phylogenetic Analysis

Formation, Transmission, and Dynamic Evolution of a Multidrug-Resistant Chromosomally Integrated Plasmid in Salmonella Spp.

IncX2 and IncX1-X2 Hybrid Plasmids Coexisting in a FosA6-Producing Escherichia coli Strain

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Characterization of a Novel IncHI2 Plasmid Carrying Tandem Copies of bla CTX-M-2 in a fosA6 -Harboring Escherichia coli Sequence Type 410 Strain

Cited by 12 publications

References 35 publications

High Genetic Plasticity in Multidrug-Resistant Sequence Type 3-IncHI2 Plasmids Revealed by Sequence Comparison and Phylogenetic Analysis

High Genetic Plasticity in Multidrug-Resistant Sequence Type 3-IncHI2 Plasmids Revealed by Sequence Comparison and Phylogenetic Analysis

Formation, Transmission, and Dynamic Evolution of a Multidrug-Resistant Chromosomally Integrated Plasmid in Salmonella Spp.

IncX2 and IncX1-X2 Hybrid Plasmids Coexisting in a FosA6-Producing Escherichia coli Strain

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Characterization of a Novel IncHI2 Plasmid Carrying Tandem Copies of bla _CTX-M-2 in a fosA6 -Harboring Escherichia coli Sequence Type 410 Strain