Characterization of a novel extracellular α-amylase from Ruminococcus bromii ATCC 27255 with neopullulanase-like activity

Jung, Jong‐Hyun; An, Yu Kyung; Son, Su Yeong; Jeong, So Young; Seo, Dong Ho; Kim, Min Kyu; Park, Cheon Seok

doi:10.1016/j.ijbiomac.2019.03.003

Cited by 18 publications

(18 citation statements)

References 35 publications

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“…Compared with SD group, diet with WS triggered marked changes in gut microbial composition, including an increase in Ruminococcus and Butyrivibrio , and a reduction in Lachnoclostridium and Ruminiclostridium . Ruminococcus is one of the key bacteria in the human colon microbiota, which is highly specific to resistant starch through the formation of amyloid [ 39 ]. It is also a microbial genus associated with age.…”

Section: Discussionmentioning

confidence: 99%

Anti-aging effects of a functional food via the action of gut microbiota and metabolites in aging mice

Zhang

Chen

et al. 2021

Aging

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Wushen (WS) is a mixed food containing 55 natural products that is beneficial to human health. This study aimed to reveal the preventive effect of WS on aging via a combined analysis of gut microbiome and metabolome. Senescence-accelerated mouse prone 8 (SAMP8) mice were used as aging model and senescence-accelerated mouse resistant 1 (SAMR1) mice as control. The mice were fed four diet types; control diet (for SAMR1 mice), standard diet (for SAMP8 mice, as SD group), WS diet, and fecal microbiota transplantation (FMT; transplanted from aging-WS mice). Our results showed that the weight, food intake, neurological function, and general physical conditions significantly improved in WS-fed mice compared to those fed with SD. The CA1 hippocampal region in WS-fed aged mice showed fewer shriveled neurons and increased neuronal layers compared to that of the SD group. WS-fed mice showed a decrease in malondialdehyde and an increase in superoxide dismutase levels in the brain; additionally, IL-6 and TNF-α levels significantly decreased, whereas IL-2 levels and the proportion of lymphocytes, CD3+CD8+ T, and CD4+IFNγ+T cells increased in WS-fed mice. After fed with WS, the abundance of Ruminococcus and Butyrivibrio markedly increased, whereas Lachnoclostridium and Ruminiclostridium significantly decreased in the aging mice. In addition, 887 differentially expressed metabolites were identified in fecal samples, among these, Butyrivibrio was positively correlated with D-glucuronic acid and Ruminococcus was positively associated with 5-acetamidovalerate. These findings provide mechanistic insight into the impact of WS on aging, and WS may be a valuable diet for preventing aging.

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Section: Discussionmentioning

confidence: 99%

Anti-aging effects of a functional food via the action of gut microbiota and metabolites in aging mice

Zhang

Chen

et al. 2021

Aging

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“…The Thermotoga maritima enzyme produces a wide range of oligosaccharides in the initial phases of digestion before breaking these products down further to the glucose and maltose product [56] characteristic of this subfamily and consistent with typical endo-acting amylase activity. However, ErAmy13B [14] and RbAmy5 [18] produce only short oligosaccharides even in the earlier stages of digestion of oligosaccharides. The tunnel-like architecture of HoAmyA would support an exo-mode of action analogous to β-amylases to generate maltose, but this seems to be absent in ErAmy13B and RbAmy5.…”

Section: Gh13_36 Activity and Product Profilesmentioning

confidence: 98%

“…the amylosome due to its lack of cohesins and dockerins, nor has it been identified proteomically as a major component of the cell-free supernatant or the cell pellet from stationary phase R. bromii cells cultured on type 3 RS or fructose [11]. The gene encoding RbAmy5 is found within a putative operon also encoding a homolog of maltose binding protein, MalE, and a putative maltose ABC transporter, MalF/G, similar to ErAmy13B [18]. Operons encoding both cell-surface glycosidases and an ABC transporter have been observed in a number of Gram positive microbes and have been termed Gram positive polysaccharide utilization locus (gpPUL) [68,69].…”

Section: Localization and Role Of Gh13_36 Enzymesmentioning

confidence: 99%

“…The GH13_20 and GH13_21 maltogenic amylases in particular contain an additional N-terminal domain (CBM34) [16] that is thought to be important for their product specificity through restriction of the active site cleft [17]. There are no CBM34 domains in recently assigned GH13_36 enzymes including ErAmy13B, SusG of Bacteroides thetaiotaomicron (BtSusG), and the secreted amylase Amy5 from Ruminococcus bromii (RbAmy5), the activity of which was recently reported [18]. Despite classification into the same subfamily, ErAmy13B, BtSusG and RbAmy5 display different activity profiles.…”

Section: Introductionmentioning

confidence: 99%

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The structures of the GH13_36 amylases from Eubacterium rectale and Ruminococcus bromii reveal subsite architectures that favor maltose production

Cockburn

Cerqueira

Bahr

et al. 2020

Amylase

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Bacteria in the human gut including Ruminococcus bromii and Eubacterium rectale encode starch-active enzymes that dictate how these bacteria interact with starch to initiate a metabolic cascade that leads to increased butyrate. Here, we determined the structures of two predicted secreted glycoside hydrolase 13 subfamily 36 (GH13_36) enzymes: ErAmy13B complexed with maltotetraose from E. rectale and RbAmy5 from R. bromii. The structures show a limited binding pocket extending from –2 through +2 subsites with limited possibilities for substrate interaction beyond this, which contributes to the propensity for members of this family to produce maltose as their main product. The enzyme structures reveal subtle differences in the +1/+2 subsites that may restrict the recognition of larger starch polymers by ErAmy13B. Our bioinformatic analysis of the biochemically characterized members of the GH13_36 subfamily, which includes the cell-surface GH13 SusG from Bacteroides thetaiotaomicron, suggests that these maltogenic amylases (EC 3.2.1.133) are usually localized to the outside of the cell, display a range of substrate preferences, and most likely contribute to maltose liberation at the cell surface during growth on starch. A broader comparison between GH13_36 and other maltogenic amylase subfamilies explain how the activity profiles of these enzymes are influenced by their structures.

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“… 83 Across five different R. bromii strains, there are 17 conserved GH13-containing amylases (Amy1-17) that enable hydrolysis of both α-1,4- and α-1,6-glycosidic bonds. 84 , 85 In addition to liberating more starch by-products (i.e. malto-oligosaccharides, maltose, and glucose) than it requires to grow, 65 R. bromii generates acetate, ethanol, formate, and propanol.…”

Section: Section 2: Resistant Starch Degradation By Microbesmentioning

confidence: 99%

Resistant starch, microbiome, and precision modulation

Dobranowski

Stintzi

2021

Gut Microbes

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Resistant starch, microbiome, and precision modulation. Mounting evidence has positioned the gut microbiome as a nexus of health. Modulating its phylogenetic composition and function has become an attractive therapeutic prospect. Resistant starches (granular amylase-resistant αglycans) are available as physicochemically and morphologically distinguishable products. Attempts to leverage resistant starch as microbiome-modifying interventions in clinical studies have yielded remarkable inter-individual variation. Consequently, their utility as a potential therapy likely depends predominantly on the selected resistant starch and the subject's baseline microbiome. The purpose of this review is to detail i) the heterogeneity of resistant starches, ii) how resistant starch is sequentially degraded and fermented by specialized gut microbes, and iii) how resistant starch interventions yield variable effects on the gut microbiome.

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Characterization of a novel extracellular α-amylase from Ruminococcus bromii ATCC 27255 with neopullulanase-like activity

Cited by 18 publications

References 35 publications

Anti-aging effects of a functional food via the action of gut microbiota and metabolites in aging mice

Anti-aging effects of a functional food via the action of gut microbiota and metabolites in aging mice

The structures of the GH13_36 amylases from Eubacterium rectale and Ruminococcus bromii reveal subsite architectures that favor maltose production

Resistant starch, microbiome, and precision modulation

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