Characterization of a novel esterase isolated from intertidal flat metagenome and its tertiary alcohols synthesis

Oh, Ki-Hoon; Nguyen, Giang-Son; Kim, Eun Young; Kourist, Robert; Bornscheuer, Uwe T.; Oh, Tae-Kwang; Yoon, Jung‐Hoon

doi:10.1016/j.molcatb.2012.04.015

Cited by 24 publications

(16 citation statements)

References 32 publications

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“…Biochemical properties, in particular pH and temperature range and stability, determine the conditions under which a specific industrial process must operate [1]. The optimal pH of RmEstB is 7.5, which is similar to that of most other reported esterases [18,35,43], higher than that of a HSL esterase from Pyrobaculum calidifontis [4] and RmEstA from R. miehei [29], and lower than that of a HSL esterase from a soil DNA library [42] and EstK from P. mandelii [39]. Most microbial esterases exhibit narrow pH stabilities under alkaline conditions [11,38,44].…”

Section: Discussionmentioning

confidence: 53%

“…It is interesting that RmEstB efficiently hydrolyzed the tertiary alcohol ester linalyl acetate to yield linalyl alcohol, similar to two esterases from metagenomic DNA libraries [42,43], while most other esterases and lipases do not possess this ability [43]. Moreover, the conversion yield (98%) is much higher than those of the other two esterases with a conversion yield of less than 50% [42,43]. This property makes this enzyme attractive for possible practical application in the production of flavor compounds in the food industry.…”

Section: Discussionmentioning

confidence: 99%

“…Esterases are widely used in organic synthesis, which often involves organic solvents [43]. Therefore, stability in organic solvents is critically important for esterases.…”

Section: Discussionmentioning

confidence: 99%

“…The production of tertiary alcohols by chemical processes generally requires extreme conditions. Enzymatic conversion provides an opportunity for more environmentally friendly routes for the production of tertiary alcohols from racemates [43]. Linalyl alcohol, one of the most important terpene alcohols that is mainly abundant in flowers, has been widely used in the fragrance and flavor industries.…”

Section: Discussionmentioning

confidence: 99%

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Characterization of a novel hormone-sensitive lipase family esterase from Rhizomucor miehei with tertiary alcohol hydrolysis activity

Yan

Yang

Duan

et al. 2014

Journal of Molecular Catalysis B: Enzymatic

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Section: Discussionmentioning

confidence: 53%

Section: Discussionmentioning

confidence: 99%

“…Esterases are widely used in organic synthesis, which often involves organic solvents [43]. Therefore, stability in organic solvents is critically important for esterases.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Characterization of a novel hormone-sensitive lipase family esterase from Rhizomucor miehei with tertiary alcohol hydrolysis activity

Yan

Yang

Duan

et al. 2014

Journal of Molecular Catalysis B: Enzymatic

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“…Six of them (Lip5.1_5, Lip5.3_3, Lip5.5_4, Lip5.13_3, Lip5.14_3, and Lip5.19_3) have been assigned to family IV. This esterase family appears to be highly represented in marine metagenomes, as out of 34 esterases identified in 8 screens of marine metagenomes, 27 have been classified as family IV esterases (39,(45)(46)(47)(48)(49)(50)(51). We have assigned one of the esterase loci identified here (Lip5.13_1) to esterase family V, which appears to count relatively few members.…”

Section: Discussionmentioning

confidence: 74%

Identification and Characterization of a Halotolerant, Cold-Active Marine Endo-β-1,4-Glucanase by Using Functional Metagenomics of Seaweed-Associated Microbiota

Martin

Biver

Steels

et al. 2014

Appl Environ Microbiol

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A metagenomic library was constructed from microorganisms associated with the brown alga Ascophyllum nodosum. Functional screening of this library revealed 13 novel putative esterase loci and two glycoside hydrolase loci. Sequence and gene cluster analysis showed the wide diversity of the identified enzymes and gave an idea of the microbial populations present during the sample collection period. Lastly, an endo-␤-1,4-glucanase having less than 50% identity to sequences of known cellulases was purified and partially characterized, showing activity at low temperature and after prolonged incubation in concentrated salt solutions. Previous surveys have revealed that less than 1% of existing microorganisms can be studied by traditional culturing methods. This leaves most microorganisms and their by-products unknown and unexploited (1, 2) and explains why metagenomics, a culture-independent approach using total microbial genomes from environmental samples, has met with great success over the past decade (3, 4). Sequence-based metagenomics has already provided information about the composition, organization, function, and hierarchy of microbial communities in particular habitats (5). On the other hand, functional genomics applied to metagenomic libraries from diverse environments has led to the discovery of many new enzymes and bioactive compounds and to substantial enrichment of genome databases. To date, most of the enzymes brought to light through metagenomics have been derived from soil (6-9) and gut (10-13) samples.Marine microorganisms represent promising candidate sources of original biocatalysts, as they are exposed to extreme conditions of temperature, pressure, salinity, nutrient availability, etc. Hence, functional screening of marine microbial populations should yield new enzymes with specific and unique physiological and biochemical properties, produced by organisms far different from those usually described in terrestrial environments (14). New enzymes have already been identified in marine metagenomic libraries from seawater samples (15, 16) and from microorganisms in symbiosis with marine organisms such as sponges and corals (17). To our knowledge, however, nobody has yet performed functional screening of metagenomic libraries from algal microbial communities. As algal microbial biofilms are in constant interaction with algal biomass, they represent an interesting source of enzymes and other active compounds (18). Sequence-based studies have already revealed the importance and functions of microbial communities living on the surfaces of algae, showing tight interdependence between algae and their biofilms (19-21). Furthermore, many genes coding for enzymes involved in hydrolyzing algal biomass, such as cellulases, xylanases, ␣-amylases, and specific algal polysaccharidases (e.g., agarases, carrageenases, and alginate lyases), have been identified from cultivable marine bacteria (22,23). Only a few such enzymes have been found in marine metagenomes by functional screening. For example, no cellulase gene...

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Strategies to Design Chemocatalytic Racemization of Tertiary Alcohols: State of the Art & Utilization for Dynamic Kinetic Resolution

Gröger,

Horino,

Kanomata

et al. 2024

Chemistry A European J

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In this "Concepts"‐article, the difficulties in racemizing tertiary alcohols are described as well as a summary of the different chemocatalytic concepts to overcome these hurdles and to pave a way for efficiently racemizing of tertiary alcohols. When combined with the enantioselective enzymatic esterification of tertiary alcohols, the racemization protocols enable a DKR to convert the racemates into enantiomerically pure esters of tertiary alcohols, which are of high interest in the field of pharmaceutically active products. Very recently, various research groups have succeeded in solving the challenging task of identifying suitable chemocatalysts for an efficient racemization of tertiary alcohols and simultaneously suppressing unwanted dehydration side‐reactions. It is noteworthy that such methods are based on different, complementary catalysis concepts. Besides Brønsted acids, oxovanadium catalysts as well as piperidine as a representative of an organocatalyst have been found to be useful for the racemization of each type of alcohols. The latter two types of catalysts also turned out to be compatible with lipase‐catalyzed kinetic resolution, thus leading to the first two examples of a proof‐of‐concept for chemoenzymatic DKR of tertiary alcohols.

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Characterization of a novel esterase isolated from intertidal flat metagenome and its tertiary alcohols synthesis

Cited by 24 publications

References 32 publications

Characterization of a novel hormone-sensitive lipase family esterase from Rhizomucor miehei with tertiary alcohol hydrolysis activity

Characterization of a novel hormone-sensitive lipase family esterase from Rhizomucor miehei with tertiary alcohol hydrolysis activity

Identification and Characterization of a Halotolerant, Cold-Active Marine Endo-β-1,4-Glucanase by Using Functional Metagenomics of Seaweed-Associated Microbiota

Strategies to Design Chemocatalytic Racemization of Tertiary Alcohols: State of the Art & Utilization for Dynamic Kinetic Resolution

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