Characterization of a Novel Diarrheagenic Strain of Proteus mirabilis Associated With Food Poisoning in China

Gong, Zelong; Shi, Xiaolu; Bai, Fang; He, Xiaolong; Zhang, Hanyun; Li, Yubin; Wan, Yu; Lin, Yiman; Qiu, Yaqun; Chen, Qiongcheng; Hu, Qinghua; Cao, Hong

doi:10.3389/fmicb.2019.02810

Cited by 42 publications

(36 citation statements)

References 38 publications

(50 reference statements)

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“…Bacillus cereus , Staphylococcus aureus, Escherichia coli, Proteus mirabilis, Klebsiella pneumoniae, Pseudomonas aeruginosa and Salmonella typhi are reported to be food borne pathogens [85] , [86] , [87] . Biosynthesized nanoparticles with its large surface area were tested for their antibacterial activity against both gram-negative and gram-positive microorganisms.…”

Section: Resultsmentioning

confidence: 99%

Synthesis, optimization and characterization of silver nanoparticles using the catkin extract of Piper longum for bactericidal effect against food-borne pathogens via conventional and mathematical approaches

Huang

Shan

Liu

et al. 2020

Bioorganic Chemistry

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Section: Resultsmentioning

confidence: 99%

Synthesis, optimization and characterization of silver nanoparticles using the catkin extract of Piper longum for bactericidal effect against food-borne pathogens via conventional and mathematical approaches

Huang

Shan

Liu

et al. 2020

Bioorganic Chemistry

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“…In Nigeria, S. uberis and S. bovis clinical mastitis are also reported by Amosun 31 . In China, the emergence of P. mirabilis as a causative agent of diarrhea was reported by Gong 32 . Moreover, in Nepal, E. faecalis diarrhea was recorded in immune-compromised persons by Sah 33 .…”

Section: Discussionmentioning

confidence: 99%

Virulence-determinants and antibiotic-resistance genes of MDR-E. coli isolated from secondary infections following FMD-outbreak in cattle

Algammal

Hetta

Batiha

et al. 2020

Sci Rep

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This study aimed to evaluate the prevalence, multidrug-resistance traits, PCR-detection of virulence, and antibiotic-resistance genes of E. coli isolated from secondary infections following FMD-outbreak in cattle. A total of 160 random samples were gathered from private dairy farms in Damietta Province, Egypt. The specimens were subjected to bacteriological examination, serotyping, congo-red binding assay, antibiogram-testing, and PCR-monitoring of virulence-determinant genes (tsh, phoA, hly, eaeA, sta, and lt) as well as the antibiotic-resistance genes (blaTEM, blaKPC, and blaCTX). The prevalence of E. coli was 30% (n = 48) distributed in 8 serogroups (40/48, 83.3%), while 8 isolates (8/48, 16.6%) were untypable. Besides, 83.3% of the examined isolates were positive for CR-binding. The tested strains harbored the virulence genes phoA, hly, tsh, eaeA, sta, and lt with a prevalence of 100% and 50%, 45.8%, 25%, 8.4%, and 6.2%, respectively. Furthermore, 50% of the recovered strains were multidrug-resistant (MDR) to penicillins, cephalosporins, and carbapenems, and are harboring the blaTEM, blaCTX, and blaKPC genes. Moreover, 25% of the examined strains are resistant to penicillins, and cephalosporins, and are harboring the blaTEM and blaCTX genes. To the best of our knowledge, this is the first report concerning the E. coli secondary bacterial infections following the FMD-outbreak. The emergence of MDR strains is considered a public health threat and indicates complicated treatment and bad prognosis of infections caused by such strains. Colistin sulfate and levofloxacin have a promising in vitro activity against MDR-E. coli.

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“…Paraffin-embedded samples after dewaxing were treated with a rabbit anti-MyD88 polyclonal antibody or a rabbit anti-NF-κB p65 polyclonal antibody (Proteintech Group, Chicago, USA) followed by being incubated with HRP-conjugated anti-rabbit (Proteintech Group, Chicago, USA) secondary antibodies. The staining index was calculated based on the proportion of positively stained cells and the intensity of staining ( 47 ).…”

Section: Methodsmentioning

confidence: 99%

Inhibitory Effects of the Lactobacillus rhamnosus GG Effector Protein HM0539 on Inflammatory Response Through the TLR4/MyD88/NF-кB Axis

Yang

Lun

et al. 2020

Front. Immunol.

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Inflammatory bowel disease (IBD) is a chronic and relapsing intestinal inflammatory condition with no effective treatment. Probiotics have gained wide attention because of their outstanding advantages in intestinal health issues. In previous studies, a novel soluble protein, HM0539, which is derived from Lactobacillus rhamnosus GG (LGG), showed significant protective effects against murine colitis, but no clear precise mechanism for this effect was provided. In this study, we hypothesized that the protective function of HM0539 might be derived from its modulation of the TLR4/Myd88/NF-κB axis signaling pathway, which is a critical pathway widely involved in the modulation of inflammatory responses. To test this hypothesis, the underlying anti-inflammatory effects and associated mechanisms of HM0539 were determined both in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages and in dextran sulfate sodium (DSS)-induced murine colitis. Our results showed that HM0539 inhibited the expression of cyclooxygenase-2 (COX-2) and the expression inducible nitric oxide synthase (iNOS) by down-regulating the activation of their respective promoter, and as a result this inhibited the production of prostaglandin E2 (PGE2) and nitric oxide (NO). Meanwhile, we demonstrated that HM0539 could ultimately modulate the activation of distal NF-κB by reducing the activation of TLR4 and suppressing the transduction of MyD88. However, even though the overexpression of TLR4 or MyD88 obviously reversed the effect of HM0539 on LPS-induced inflammation, HM0539 still retained some anti-inflammatory activity. Consistent with the in vitro findings, we found that HM0539 inhibited to a great extent the production of inflammatory mediators associated with the suppression of the TLR4/Myd88/NF-κB axis activation in colon tissue. In conclusion, HM0539 was shown to be a promising anti-inflammatory agent, at least in part through its down-regulation of the TLR4-MyD88 axis as well as of the downstream MyD88-dependent activated NF-κB signaling, and hence might be considered as a potential therapeutic option for IBD.

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Characterization of a Novel Diarrheagenic Strain of Proteus mirabilis Associated With Food Poisoning in China

Cited by 42 publications

References 38 publications

Synthesis, optimization and characterization of silver nanoparticles using the catkin extract of Piper longum for bactericidal effect against food-borne pathogens via conventional and mathematical approaches

Synthesis, optimization and characterization of silver nanoparticles using the catkin extract of Piper longum for bactericidal effect against food-borne pathogens via conventional and mathematical approaches

Virulence-determinants and antibiotic-resistance genes of MDR-E. coli isolated from secondary infections following FMD-outbreak in cattle

Inhibitory Effects of the Lactobacillus rhamnosus GG Effector Protein HM0539 on Inflammatory Response Through the TLR4/MyD88/NF-кB Axis

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