Characterization of a Novel Coprothermobacter sp. Strain IT3 Isolated from an Anaerobic Digester

Tandishabo, Kalayi; Iga, Yoshimi; Tamaki, Hideyuki; Nakamura, Kohei; Takamizawa, Kazuhiro

doi:10.5956/jriet.41.753

Cited by 6 publications

(1 citation statement)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…were found to have strong intracellular and extracellular protease activity capable of protein and peptide degradation. Additionally, they were identified in anaerobic systems as important hydrogen producers (Tandishabo et al, 2012). Development of mixed populations of species belonging to Firmicutes, Synergistetes, Coprothermobacteria , and Anaerolineae were observed in microbial fuel cell (MFC) fed with acetate (Fu et al, 2013) and of root exudate-driven MFCs (Cabezas et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Temporal Microbial Community Dynamics in Microbial Electrolysis Cells – Influence of Acetate and Propionate Concentration

et al. 2017

View full text Add to dashboard Cite

Microbial electrolysis cells (MECs) are widely considered as a next generation wastewater treatment system. However, fundamental insight on the temporal dynamics of microbial communities associated with MEC performance under different organic types with varied loading concentrations is still unknown, nevertheless this knowledge is essential for optimizing this technology for real-scale applications. Here, the temporal dynamics of anodic microbial communities associated with MEC performance was examined at low (0.5 g COD/L) and high (4 g COD/L) concentrations of acetate or propionate, which are important intermediates of fermentation of municipal wastewaters and sludge. The results showed that acetate-fed reactors exhibited higher performance in terms of maximum current density (I: 4.25 ± 0.23 A/m2), coulombic efficiency (CE: 95 ± 8%), and substrate degradation rate (98.8 ± 1.2%) than propionate-fed reactors (I: 2.7 ± 0.28 A/m2; CE: 68 ± 9.5%; substrate degradation rate: 84 ± 13%) irrespective of the concentrations tested. Despite of the repeated sampling of the anodic biofilm over time, the high-concentration reactors demonstrated lower and stable performance in terms of current density (I: 1.1 ± 0.14 to 4.2 ± 0.21 A/m2), coulombic efficiency (CE: 44 ± 4.1 to 103 ± 7.2%) and substrate degradation rate (64.9 ± 6.3 to 99.7 ± 0.5%), while the low-concentration reactors produced higher and dynamic performance (I: 1.1 ± 0.12 to 4.6 ± 0.1 A/m2; CE: 52 ± 2.5 to 105 ± 2.7%; substrate degradation rate: 87.2 ± 0.2 to 99.9 ± 0.06%) with the different substrates tested. Correlating reactor’s performance with temporal dynamics of microbial communities showed that relatively similar anodic microbial community composition but with varying relative abundances was observed in all the reactors despite differences in the substrate and concentrations tested. Particularly, Geobacter was the predominant bacteria on the anode biofilm of all MECs over time suggesting its possible role in maintaining functional stability of MECs fed with low and high concentrations of acetate and propionate. Taken together, these results provide new insights on the microbial community dynamics and its correlation to performance in MECs fed with different concentrations of acetate and propionate, which are important volatile fatty acids in wastewater.

show abstract

Section: Discussionmentioning

confidence: 99%

Temporal Microbial Community Dynamics in Microbial Electrolysis Cells – Influence of Acetate and Propionate Concentration

et al. 2017

View full text Add to dashboard Cite

show abstract

C oprothermobacter

Paván¹,

Pavan²,

Kämpfer³

et al. 2019

Bergey's Manual of Systematics of Archaea and Bacteria

View full text Add to dashboard Cite

Co.pro.ther.mo.bac'ter. Gr. fem. n. kopros manure; Gr. adj. thermos warm; N.L. masc. n. bacter a rod; N.L. masc. n. Coprothermobacter a thermophilic rod from manure. Coprothermobacterota / Coprothermobacteria / Coprothermobacterales / Coprothermobacteraceae / Coprothermobacter Rod‐shaped cells, 0.5 × 1.0–6.0 µm; occur singly or in pairs in young cultures; pleomorphic in old cultures. Colonies are white, circular (diameter 1–2 mm), convex, and smooth with entire edges. Stains Gram‐negative. Non‐spore‐forming. Nonmotile. Strictly anaerobic. Thermophilic. Optimum growth temperature, 55–70°C. Neutrophilic. Chemoorganotrophic. Proteolytic, uses gelatin and peptones. Sugars are used poorly unless yeast extract or rumen fluid is added. Trypticase peptone stimulates glucose utilization. The main end products of sugar or protein fermentation are acetic acid, H 2 , and CO 2 . Thiosulfate, but not sulfate, is used as electron acceptor for growth. Dominant in thermophilic anaerobic digesters, also present in marine and terrestrial environments. The microorganisms of the genus Coprothermobacter represent one of the primary lines of descent in the bacterial domain. DNA G + C content (mol%) : 42.5–44.8 (genome analysis). Type species : Coprothermobacter proteolyticus (Ollivier, Mah, Ferguson, Boone, Garcia and Robinson 1985) Rainey and Stackebrandt 1993a, 858 VP ( Thermobacteroides proteolyticus Ollivier, Mah, Ferguson, Boone, Garcia and Robinson 1985, 427).

show abstract

Recovering the sulfidogenic activity under harsh sulfide-rich fermentative conditions in a thermophilic structured-bed reactor: Performance and microbial aspects

Gil-Garcia

Fuess

Rabelo

et al. 2023

Environmental Technology & Innovation

View full text Add to dashboard Cite

Characterization of a Novel Coprothermobacter sp. Strain IT3 Isolated from an Anaerobic Digester

Cited by 6 publications

References 10 publications

Temporal Microbial Community Dynamics in Microbial Electrolysis Cells – Influence of Acetate and Propionate Concentration

Temporal Microbial Community Dynamics in Microbial Electrolysis Cells – Influence of Acetate and Propionate Concentration

C oprothermobacter

Recovering the sulfidogenic activity under harsh sulfide-rich fermentative conditions in a thermophilic structured-bed reactor: Performance and microbial aspects

Contact Info

Product

Resources

About