Characterization of a Novel Carbapenem-Resistant Klebsiella michiganensis Strain Coharboring the blaSIM-1, blaOXA-1, blaCTX-M-14, qnrS, and aac(6′)-Ib-cr Genes

Xu, Jianfen; Ding, Hui; Zhao, Yunan; Chen, Jiaoli; Zhao, Zhigang; Huang, Jiansheng; Wu, Rongzhen

doi:10.1007/s00284-022-02920-7

Cited by 2 publications

(1 citation statement)

References 26 publications

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“…Carbapenemase production is the common carbapenemase resistance mechanism in clinical infections, such as Acinetobacter baumannii ( Oinuma et al, 2021 ), K. pneumoniae ( Pitout et al, 2015 ) and Pseudomonas aeruginosa ( Nordmann et al, 2011 ; Doi and Paterson, 2015 ; Iovleva and Doi, 2017 ). The main carbapenemase genes carried by K. michiganensis include bla KPC-2 , bla VIM-1 ( Campos-Madueno et al, 2021 ), bla NDM-1 ( Li et al, 2021 )， bla NDM-5 ( Zheng et al, 2018 ), bla KPC-3 ( Seiffert et al, 2019 ), bla IMP-4 ( Li et al, 2021 ; Zhang et al, 2021 ), bla OXA-181 ( Founou et al, 2018 )， bla SIM-1 , bla OXA-1 , bla CTX-M-14 ( Xu et al, 2022 ), etc.…”

Section: Introductionmentioning

confidence: 99%

A clinical KPC-producing Klebsiella michiganensis strain carrying IncFII/IncFIA (HI1)/IncFIB (K) multiple replicon plasmid

Zhang

Liu²,

Qi³

et al. 2023

Front. Microbiol.

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Klebsiella michiganensis is an increasingly important bacterial pathogen causing nosocomial infections in clinical patients. In this study, we described the molecular and genomic characteristics of a carbapenem-resistant K. michiganensis strain KM166 cultured from a one-month premature baby’s blood sample. KM166 showed lower biofilm forming ability in optical density (OD) than K. pneumoniae NTUH-K2044 (0.271 ± 0.027 vs. 0.595 ± 0.054, p = 0.001), and the median lethal dose (0.684 lg CFU/mL) was lower than K. pneumoniae strain NTUH-K2044 (6.679 lg CFU/mL). A IncFII/IncFIA(HI1)/IncFIB(K) multiple replicon plasmid in KM166 was identified carrying three replicon types. It has low homology to Escherichia coli pMRY09-581ECO_1 and the highest homology similarity to the INcFIA/INcFII(p14)-type plasmid in K. michiganensis strain fxq plasmid pB_KPC, suggesting that this multiple replicon plasmid was unlikely to have been transmitted from E. coli and probably a transfer of repFIB replicon genes from other K. michiganensis strains into the INcFIA/INcFII(p14)-type plasmid of KM166 had occurred. Mapping of the gene environment revealed that blaKPC-2 in KM166 plasmid 3 had high identity and same Tn3-tnpR-IS481-blaKPC-2-klcA_1 genomic context structure with K. pneumoniae strain JKP55, plasmid pKPC-J5501, and blaKPC-2-carrying plasmid proved to be autonomously transferred under the help of mobile genetic elements into Escherichia coli 600 by plasmid conjugation experiment. In conclusion, we have characterized a K. michiganensis strain carrying multi-replicon IncFII/IncFIA(HI1)/IncFIB(K) plasmid and blaKPC-2-carrying IncFII(p14)/IncFIA plasmid in this study, which provided insights about the evolutionary diversity of plasmids carried by K. michiganensis.

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Section: Introductionmentioning

confidence: 99%

A clinical KPC-producing Klebsiella michiganensis strain carrying IncFII/IncFIA (HI1)/IncFIB (K) multiple replicon plasmid

Zhang

Liu²,

Qi³

et al. 2023

Front. Microbiol.

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Genomic and functional analysis of high-level tigecycline resistant Klebsiella michiganensis co-carrying tet(X4) and tmexCD2-toprJ2 from pork

et al. 2023

International Journal of Food Microbiology

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Characterization of a Novel Carbapenem-Resistant Klebsiella michiganensis Strain Coharboring the blaSIM-1, blaOXA-1, blaCTX-M-14, qnrS, and aac(6′)-Ib-cr Genes

Cited by 2 publications

References 26 publications

A clinical KPC-producing Klebsiella michiganensis strain carrying IncFII/IncFIA (HI1)/IncFIB (K) multiple replicon plasmid

A clinical KPC-producing Klebsiella michiganensis strain carrying IncFII/IncFIA (HI1)/IncFIB (K) multiple replicon plasmid

Genomic and functional analysis of high-level tigecycline resistant Klebsiella michiganensis co-carrying tet(X4) and tmexCD2-toprJ2 from pork

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