Characterization of a novel bacteriophage, Phda1, infecting the histamine-producing <i>Photobacterium damselae</i>
 subsp. <i>damselae</i>

Yamaki, Shogo; Kawai, Yuji; Yamazaki, Koji

doi:10.1111/jam.12809

Cited by 13 publications

(9 citation statements)

References 37 publications

(66 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a pathogen that also thrives as a free-living bacterium in seawater, P. damselae subsp. damselae lives in close contact with bacteriophages ( Novianty et al, 2014 ; Yamaki et al, 2015 ), hence the abundance of CRISPR-Cas systems. Not surprisingly, much of the variable DNA content that showed to be specific of each of the four genomes analyzed here contained typical features of prophage DNA.…”

Section: Discussionmentioning

confidence: 99%

Molecular Epidemiology of Photobacterium damselae subsp. damselae Outbreaks in Marine Rainbow Trout Farms Reveals Extensive Horizontal Gene Transfer and High Genetic Diversity

et al. 2018

View full text Add to dashboard Cite

The marine bacterium Photobacterium damselae subsp. damselae is a pathogen for a variety of marine animals, as well as for humans, and is nowadays considered an emerging pathogen for fish of importance in marine aquaculture. Recent studies have suggested that outbreaks in fish farms are caused by multiclonal populations of this subspecies that exist in the environment. Here, we report the study of a collection of 31 strains isolated during the course of disease outbreaks in marine rainbow trout farms in Denmark in 1994, 1995, and 2006, respectively. A phylogenetic analysis based on the toxR gene sequence, and the screening of virulence-related genes uncovered a high genetic heterogeneity, even among strains isolated from the same fish farm at the same time. Moreover, comparative analysis of the whole genome sequences of four selected strains revealed a large number of differentially occurring genes, which included virulence genes, pPHDD1 plasmid, polysaccharide synthesis gene clusters, CRISPR-Cas systems and putative new mobile genetic elements. This study provides sound evidence that P. damselae subsp. damselae outbreaks in Danish rainbow trout farms were caused by multiclonal populations and that horizontal gene transfer constitutes a strong driving force in the generation of intraspecific diversity in this pathogen.

show abstract

Section: Discussionmentioning

confidence: 99%

Molecular Epidemiology of Photobacterium damselae subsp. damselae Outbreaks in Marine Rainbow Trout Farms Reveals Extensive Horizontal Gene Transfer and High Genetic Diversity

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Phages also afford other advantages as biocontrol agents: they are harmless to humans, animals and plants, have a long shelf life if stored correctly, are able to resist the environmental stresses encountered during food processing (as well as the physiochemical conditions of food), are relatively cheap and easy to isolate and propagate, and since they are self-replicating and self-limiting, low dosages can be employed since they multiply only, and as long as the host is present ( Sillankorva et al, 2012 ). They have been proposed as a means of eliminating food-borne pathogens such as Listeria , Staphylococci , and enterotoxigenic Escherichia coli strains ( Garcia et al, 2008 ), but they might also be used against food spoilage microorganisms ( Deasy et al, 2011 ; Yamaki et al, 2015 ). The use of phages that infect BA-producing LAB in fermented foods has, to our knowledge, not been proposed.…”

Section: Introductionmentioning

confidence: 99%

Q69 (an E. faecalis-Infecting Bacteriophage) As a Biocontrol Agent for Reducing Tyramine in Dairy Products

et al. 2016

View full text Add to dashboard Cite

Biogenic amines (BAs) are low molecular weight nitrogenous compounds with biological activity, formed from amino acids by decarboxylation. BAs are naturally present in all living organisms playing essential roles. However, their accumulation in food through the metabolic activity of certain microorganisms constitutes a toxicological hazard. Among foods, cheeses accumulate some of the highest concentrations of BAs since they provide an ideal environment for their accumulation. Most of the methods proposed for reducing BAs in cheese, such as milk pasteurization, have not only failed to completely solve the problem, they also affect non-BA producing lactic acid bacteria, i.e., the bacteria that participate in the development of the organoleptic characteristics of cheese. Novel technologies specifically targeted against BA producers are therefore needed to control BA accumulation. Bacteriophages have been proposed as agents for specifically controlling the presence of foodborne pathogens in food. Due to its specificity, they could be used as a biotechnological tool targeted to reduce the population of BA-producing bacteria. The present work reports the isolation, from cheese, and the characterization of bacteriophage Q69, which infects specifically Enterococcus faecalis, the species mainly responsible of the accumulation of the BA tyramine in foods. Furthermore, its capacity to reduce the accumulation of tyramine in different conditions –including a model cheese- was proven. The obtained results open up the possibility of use bacteriophages to prevent BA accumulation in fermented foods.

show abstract

“…2010; Yamaki et al . 2015; Matamp and Bhat 2019). A positive theoretical correlation is assumed between lysis time and burst size, that is, the longer the lysis time, the larger the burst size.…”

Section: Discussionmentioning

confidence: 99%

Use of bacteriophage vB_Pd_PDCC‐1 as biological control agent of Photobacterium damselae subsp. damselae during hatching of longfin yellowtail ( Seriola rivoliana ) eggs

et al. 2020

View full text Add to dashboard Cite

Aims This study describes the effect of phage therapy on hatching of longfin yellowtail (Seriola rivoliana) eggs challenged with Photobacterium damselae subsp. damselae. Methods and results A lytic phage (vB_Pd_PDCC‐1) against P. damselae subsp. damselae was isolated and characterized. The use of phage vB_Pd_PDCC‐1 increased the hatching rate of eggs, and reduced presumptive Vibrio species to non‐detectable numbers, even in non‐disinfected eggs. High‐throughput 16S rRNA gene sequencing analysis revealed that phage vB_Pd_PDCC‐1 caused significant changes in the composition and structure of the associated microbiota, allowing that members (e.g. those belonging to the family Vibrionaceae) of the class Gammaproteobacteria to be displaced by members of the class Alphaproteobacteria. Conclusions To the best of our knowledge, this represents the first study evaluating phage therapy to control potential negative effects of P. damselae subsp. damselae during hatching of longfin yellowtail eggs. Significance and Impact of the Study The Seriola genus includes several important commercial fish species due to its rapid growth and easy adaptability to confinement conditions. However, bacterial infections (especially those caused by Vibrio and Photobacterium species) are among the main limiting factors for the intensification of marine fish aquaculture, particularly during early development stages. Therefore, the use of phages, which are natural killers of bacteria, represents a promising strategy to reduce the mortality of farmed organisms caused by pathogenic bacteria.

show abstract

Characterization of a novel bacteriophage, Phda1, infecting the histamine-producing Photobacterium damselae subsp. damselae

Abstract: This is the first description of a bacteriophage specifically infecting P. damselae subsp. damselae and its potential applications. Bacteriophage therapy could prove useful in the prevention of histamine poisoning.

Cited by 13 publications

References 37 publications

Molecular Epidemiology of Photobacterium damselae subsp. damselae Outbreaks in Marine Rainbow Trout Farms Reveals Extensive Horizontal Gene Transfer and High Genetic Diversity

Molecular Epidemiology of Photobacterium damselae subsp. damselae Outbreaks in Marine Rainbow Trout Farms Reveals Extensive Horizontal Gene Transfer and High Genetic Diversity

Q69 (an E. faecalis-Infecting Bacteriophage) As a Biocontrol Agent for Reducing Tyramine in Dairy Products

Use of bacteriophage vB_Pd_PDCC‐1 as biological control agent of Photobacterium damselae subsp. damselae during hatching of longfin yellowtail ( Seriola rivoliana ) eggs

Contact Info

Product

Resources

About