Characterization of a novel Bacillus thuringiensis toxin active against Aedes aegypti larvae

Wu, Jiangyu; Li, Wei; He, Jiali; Fu, Kang; Li, Xinxin; Luyu, Jia; Wang, Ruiping; Zhang, Wenfei

doi:10.1016/j.actatropica.2021.106088

Cited by 9 publications

(4 citation statements)

References 28 publications

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“…The toxins of Bti and Lbs and the mechanisms associated with mosquito mortality have been extensively studied [ 32 , 33 ]. Briefly, in Bti, the molecules responsible for the entomopathogenic action are mainly the crystal toxins Cry4Aa, Cry4Ba, Cry10Aa, and Cry11Aa, the cytolytic toxins Cyt1Aa, and Cyt2Ba, and the P19 and P20 proteins [ 34 , 35 ]. Cry and Cyt toxins, also known as δ-endotoxins, when present in the midgut of mosquito larvae, are proteolytically activated by digestive proteases, bind to specific receptors on the host cell membranes, and cause cell rupture resulting in the death of infected larvae [ 36 ].…”

Section: Bacteria For Biological Control Of Medically Important Mosqu...mentioning

confidence: 99%

Biotechnological Potential of Microorganisms for Mosquito Population Control and Reduction in Vector Competence

Katak,

Cintra,

Burini

et al. 2023

Insects

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Mosquitoes transmit pathogens that cause human diseases such as malaria, dengue fever, chikungunya, yellow fever, Zika fever, and filariasis. Biotechnological approaches using microorganisms have a significant potential to control mosquito populations and reduce their vector competence, making them alternatives to synthetic insecticides. Ongoing research has identified many microorganisms that can be used effectively to control mosquito populations and disease transmission. However, the successful implementation of these newly proposed approaches requires a thorough understanding of the multipronged microorganism–mosquito–pathogen–environment interactions. Although much has been achieved in discovering new entomopathogenic microorganisms, antipathogen compounds, and their mechanisms of action, only a few have been turned into viable products for mosquito control. There is a discrepancy between the number of microorganisms with the potential for the development of new insecticides and/or antipathogen products and the actual available products, highlighting the need for investments in the intersection of basic research and biotechnology.

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Section: Bacteria For Biological Control Of Medically Important Mosqu...mentioning

confidence: 99%

Biotechnological Potential of Microorganisms for Mosquito Population Control and Reduction in Vector Competence

Katak,

Cintra,

Burini

et al. 2023

Insects

View full text Add to dashboard Cite

show abstract

“…Improvements in the control of mosquitoes using Bt have involved the constant identification and characterization of novel strains and pesticidal proteins [9][10][11][12]. In addition, next-generation sequencing (NGS) has allowed the whole-genome sequencing of novel Bt strains and their characterization.…”

Section: Introductionmentioning

confidence: 99%

Isolation, Genomic, and Proteomic Characterization of a Novel Neotropical Strain of Bacillus thuringiensis with Mosquitocidal Activities

et al. 2023

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The combination of genomic and proteomic analyses is a useful tool for the study of novel Bacillus thuringiensis (Bt) strains, as these approaches allow the accurate identification of pesticidal proteins and virulence factors produced. Here, we isolated and evaluated the potential of a novel Neotropical Bt strain (TOD651) for controlling larvae of Aedes aegypti and Culex quinquefasciatus mosquitoes. Aiming for the full comprehension of the TOD651 larvicidal potential, we further evaluated the whole TOD651 genome and conducted the proteomic analysis of the TOD651 spore–crystal mixtures. Our results showed that Bt TOD651 similarly killed both A. aegypti (0.011 µg/mL) and C. quinquefasciatus (0.023 µg/mL) larvae, exhibiting similar potency to the commercial Bt strain. The genome sequence revealed that Bt TOD651 harbors cry11Aa3, cry10Aa4, cry4Aa4, cry4Ba5, cyt1Aa5, cyt1Ca1, cyt2Ba13, mpp60Aa3, and mpp60Ba3. The proteomic analysis revealed no expression of Mpp60Aa3, while all the other pesticidal proteins were expressed (Cry4Ba5 was more abundant than Cyt1Aa5). The expression of the Mppe showed the major proportions between proteases. The virulent factor neutral protease B and spore coat proteins were also expressed. The expression of relevant pesticidal proteins (e.g., Cry, Cyt, Mpp, and other pathogenic factors), whose actions can occur in a synergic relation, indicates that the biocontrol using Bt TOD651 may contribute to delaying the selection of resistant individuals.

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“…Bt produces a number of crystal proteins that have insecticidal activity against over 3000 insect species, including Coleoptera, Lepidoptera, and Dipterans (18)(19)(20). These toxin proteins, including Cry4Aa, Cry4Ba, Cry10Aa, Cry11Aa, and Cyt1Aa, are toxic to mosquitoes (21)(22)(23). Cry toxin's effectiveness against insect pests is dependent on their interactions with other receptors such as alkaline phosphatase (ALP), aminopeptidase-N (APN), cadherin (CAD), and ATPbinding cassette (ABC) transporters (24)(25)(26)(27)(28)(29)(30)(31).…”

Section: Introductionmentioning

confidence: 99%

“…For that reason, it is crucial to comprehend the interactions between Cry toxins and other midgut proteins. In addition to Cry toxins, Cyt toxins are important for inducing toxicity in some insect orders (23,32,33). For example, the Bt strain LLP29 produces the Cyt1Aa6 toxin, which is toxic to Ae.…”

Section: Introductionmentioning

confidence: 99%

Role of Lectin in the Response of Aedes aegypti Against Bt Toxin

et al. 2022

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Aedes aegypti is one of the world’s most dangerous mosquitoes, and a vector of diseases such as dengue fever, chikungunya virus, yellow fever, and Zika virus disease. Currently, a major global challenge is the scarcity of antiviral medicine and vaccine for arboviruses. Bacillus thuringiensis var israelensis (Bti) toxins are used as biological mosquito control agents. Endotoxins, including Cry4Aa, Cry4Ba, Cry10Aa, Cry11Aa, and Cyt1Aa, are toxic to mosquitoes. Insect eradication by Cry toxin relies primarily on the interaction of cry toxins with key toxin receptors, such as aminopeptidase (APN), alkaline phosphatase (ALP), cadherin (CAD), and ATP-binding cassette transporters. The carbohydrate recognition domains (CRDs) of lectins and domains II and III of Cry toxins share similar structural folds, suggesting that midgut proteins, such as C-type lectins (CTLs), may interfere with interactions among Cry toxins and receptors by binding to both and alter Cry toxicity. In the present review, we summarize the functional role of C-type lectins in Ae. aegypti mosquitoes and the mechanism underlying the alteration of Cry toxin activity by CTLs. Furthermore, we outline future research directions on elucidating the Bti resistance mechanism. This study provides a basis for understanding Bti resistance, which can be used to develop novel insecticides.

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Characterization of a novel Bacillus thuringiensis toxin active against Aedes aegypti larvae

Cited by 9 publications

References 28 publications

Biotechnological Potential of Microorganisms for Mosquito Population Control and Reduction in Vector Competence

Biotechnological Potential of Microorganisms for Mosquito Population Control and Reduction in Vector Competence

Isolation, Genomic, and Proteomic Characterization of a Novel Neotropical Strain of Bacillus thuringiensis with Mosquitocidal Activities

Role of Lectin in the Response of Aedes aegypti Against Bt Toxin

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