Characterization of a NewAedes albopictus(Diptera: Culicidae)-Wolbachia pipientis(Rickettsiales: Rickettsiaceae) Symbiotic Association Generated by Artificial Transfer of thewPip Strain FromCulex pipiens(Diptera: Culicidae)

Calvitti, Maurizio; Moretti, Riccardo; López-Knowles, Elena; Bellini, Romeo; Dobson, Stephen L.

doi:10.1093/jmedent/47.2.179

Cited by 40 publications

(69 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies indicate fecundity effects of W. pipientis vary widely. Some studies suggest there is no effect of W. pipientis on fecundity under laboratory conditions in D. melanogaster Reynolds and Hoffmann 2002) or in N. vitripennis (Bordenstein and Werren 2000), while others have found harmful effects (Hoffmann et al 1990;Nigro 1991;Stouthamer and Luck 1993;Calviti et al 2010). In our study, a decrease in fecundity was found in the high-density IntG12.1 N. giraulti females.…”

Section: Discussionsupporting

confidence: 43%

Decoupling of Host–Symbiont–Phage Coadaptations Following Transfer Between Insect Species

et al. 2011

View full text Add to dashboard Cite

Transferring endosymbiotic bacteria between different host species can perturb the coordinated regulation of the host and bacterial genomes. Here we use the most common maternally transmitted bacteria, Wolbachia pipientis, to test the consequences of host genetic background on infection densities and the processes underlying those changes in the parasitoid wasp genus Nasonia. Introgressing the genome of Nasonia giraulti into the infected cytoplasm of N. vitripennis causes a two-order-of-magnitude increase in bacterial loads in adults and a proliferation of the infection to somatic tissues. The host effect on W. pipientis distribution and densities is associated with a twofold decrease in densities of the temperate phage WO-B. Returning the bacteria from the new host species back to the resident host species restores the bacteria and phage to their native densities. To our knowledge, this is the first study to report a hostmicrobe genetic interaction that affects the densities of both W. pipientis and bacteriophage WO-B. The consequences of the increased bacterial density include a reduction in fecundity, an increase in levels of cytoplasmic incompatibility (CI), and unexpectedly, male-to-female transfer of the bacteria to uninfected females and an increased acceptance of densely infected females to interspecific mates. While paternal inheritance of the W. pipientis was not observed, the high incidence of male-to-female transfer in the introgressed background raises the possibility that paternal transmission could be more likely in hybrids where paternal leakage of other cytoplasmic elements is also known to occur. Taken together, these results establish a major change in W. pipientis densities and tissue tropism between closely related species and support a model in which phage WO, Wolbachia, and arthropods form a tripartite symbiotic association in which all three are integral to understanding the biology of this widespread endosymbiosis.

show abstract

Section: Discussionsupporting

confidence: 43%

Decoupling of Host–Symbiont–Phage Coadaptations Following Transfer Between Insect Species

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Our results so far show no major effect on fecundity, unlike the significant fecundity reduction previously observed with wPip infection of Ae. albopictus (37). Furthermore, our wMel-transinfected line had a much higher hatch rate than that previously observed for a wMelPop strain transinfection in Ae.…”

Section: Daycontrasting

confidence: 40%

WolbachiastrainwMel induces cytoplasmic incompatibility and blocks dengue transmission inAedes albopictus

Blagrove

Arias-Goeta

Failloux

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

286

260

View full text Add to dashboard Cite

Wolbachia inherited bacteria are able to invade insect populations using cytoplasmic incompatibility and provide new strategies for controlling mosquito-borne tropical diseases, such as dengue. The overreplicating wMelPop strain was recently shown to strongly inhibit the replication of dengue virus when introduced into Aedes aegypti mosquitoes, as well as to stimulate chronic immune upregulation. Here we show that stable introduction of the wMel strain of Drosophila melanogaster into Aedes albopictus, a vector of dengue and other arboviruses, abolished the transmission capacity of dengue virus-challenged mosquitoes. Immune up-regulation was observed in the transinfected line, but at a much lower level than that previously found for transinfected Ae. aegypti. Transient infection experiments suggest that this difference is related to Ae. albopictus immunotolerance of Wolbachia, rather than to the Wolbachia strain used. This study provides an example of strong pathogen inhibition in a naturally Wolbachia-infected mosquito species, demonstrating that this inhibition is not limited to naturally naïve species, and suggests that the Wolbachia strain is more important than host background for viral inhibition. Complete bidirectional cytoplasmic incompatibility was observed with WT strains infected with the naturally occurring Ae. albopictus Wolbachia, and this provides a mechanism for introducing wMel into natural populations of this species.

show abstract

“…Infection by Wolbachia decreases the fecundity of host insects, and releasing transinfected insects with low fecundity into the field may suppress the pest population (29)(30)(31). In B. longissima, egg production is higher in the Asian clade than in the Pacific clade (32).…”

Section: Discussionmentioning

confidence: 99%

Unique clade of alphaproteobacterial endosymbionts induces complete cytoplasmic incompatibility in the coconut beetle

Takano

Tuda

Takasu

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Maternally inherited bacterial endosymbionts in arthropods manipulate host reproduction to increase the fitness of infected females. Cytoplasmic incompatibility (CI) is one such manipulation, in which uninfected females produce few or no offspring when they mate with infected males. To date, two bacterial endosymbionts, Wolbachia and Cardinium, have been reported as CI inducers. Only Wolbachia induces complete CI, which causes 100% offspring mortality in incompatible crosses. Here we report a third CI inducer that belongs to a unique clade of Alphaproteobacteria detected within the coconut beetle, Brontispa longissima. This beetle comprises two cryptic species, the Asian clade and the Pacific clade, which show incompatibility in hybrid crosses. Different bacterial endosymbionts, a unique clade of Alphaproteobacteria in the Pacific clade and Wolbachia in the Asian clade, induced bidirectional CI between hosts. The former induced complete CI (100% mortality), whereas the latter induced partial CI (70% mortality). Illumina MiSeq sequencing and denaturing gradient gel electrophoresis patterns showed that the predominant bacterium detected in the Pacific clade of B. longissima was this unique clade of Alphaproteobacteria alone, indicating that this endosymbiont was responsible for the complete CI. Sex distortion did not occur in any of the tested crosses. The 1,160 bp of 16S rRNA gene sequence obtained for this endosymbiont had only 89.3% identity with that of Wolbachia, indicating that it can be recognized as a distinct species. We discuss the potential use of this bacterium as a biological control agent.

show abstract

Characterization of a NewAedes albopictus(Diptera: Culicidae)-Wolbachia pipientis(Rickettsiales: Rickettsiaceae) Symbiotic Association Generated by Artificial Transfer of thewPip Strain FromCulex pipiens(Diptera: Culicidae)

Cited by 40 publications

References 46 publications

Decoupling of Host–Symbiont–Phage Coadaptations Following Transfer Between Insect Species

Decoupling of Host–Symbiont–Phage Coadaptations Following Transfer Between Insect Species

WolbachiastrainwMel induces cytoplasmic incompatibility and blocks dengue transmission inAedes albopictus

Unique clade of alphaproteobacterial endosymbionts induces complete cytoplasmic incompatibility in the coconut beetle

Contact Info

Product

Resources

About