Characterization of a new hybrid mink-mouse clone panel: chromosomal and regional assignments of the GLO, ACY, NP, CKBB, ADH2, and ME1 loci in mink (Mustela vison)

Abstract

To expand the mink map, we established a new panel consisting of 23 mink-mouse clones. On the basis of statistical criteria (Wijnen et al. 1977; Burgerhout 1978), we developed a computer program for choice of clones of the panel. Assignments of the following mink genes were achieved with the use of the hybrid panel: glyoxalase (GLO), Chromosome (Chr) 1; acetyl acylase (ACY), Chr 5; creatine phosphokinase B (CKBB), Chr 10; alcohol dehydrogenase-2 (subunit B) (ADH2), Chr 8. Using a series of clones carrying rearrangements involving mink Chr 1 and 8, we assigned the gene for ME1 to the short arm of Chr 1 and that for ADH2 to Chr 8, in the region 8p12-p24. Mapping results confirm the ones we previously obtained with a mink-Chinese hamster panel. However, by means of an improved electrophoretic technique, we revised the localization of the gene for purine nucleoside phosphorylase (NP), which has been thought to be on mink Chr 2. It is reassigned to mink Chr 10.

A comparative, BAC end sequence enabled map of the genome of the American mink (Neovison vison)

“…To date, the focus has been on coat color genetics (Shackelford, 1948(Shackelford, & 1949Ness et al, 1988;Trapezov, 1997;Anistoroaei et al, & 2008Benkel et al, 2009), isolating microsatellite markers and constructing linkage maps (Anistoroaei et al, 2006Ansari et. al, 2007), and gene mapping using Zoo-FISH experiments (Hameister et al, 1997;Graphodatsky et al, 2000;Kuznetsov et al, 2003) and somatic cell hybrids (Rubtsov et al, 1981;Pack et al, 1992).…”

Construction of a cytogenetically characterized porcine somatic cell hybrid panel and its use as a mapping tool

“…Presence (+) or absence (-) of porcine-specific PCR amplification products of 12 microsatellites in the 21 hybrids of the panel. A weak PCR band is indicated by • Hybrid S0022 S0023 S0084 S0098 S0112 S0113 S0114 S0115 S0117 S0118 S0119 S0120 wg3b 8607 02 ---+ .... + ---8607 04 -+ -+ --+ -+ -+ -8607 10 -+ + + + + -+ + ---8607 11 - for instance Szpirer et al 1984;Pack et al 1992;Deng et al 1994;Rettenberger et al 1994;Zhdanova et al 1994;Zhang et al 1995).…”

70 years of research on the American mink (Neovison vison schreb., 1777) genetics - where are we now?

“…The chromosomal location of at least 127 genes of the American mink has been established (KUZNETSOV et al, 2003;BENSON et al, 2012). Gene mapping is based here on the use of somatic cellular hybrids of the American mink-Chinese hamster (Cricetulus griseus Milne-Edwards, 1867) and the American mink-house mouse (Mus musculus L., 1758) (RUBSTOV et al, 1981;PACK et al, 1992;KUZNETSOV et al, 2003), methods of comparative cytogenetics -FISH (Fluorescence in situ Hybridization), Zoo-FISH (Cross-Species Fluorescent in situ Hybridization), comparative gene mapping and breeding tests (SEROV, 1998;SEROV and RUBSTOV, 1998). It is worth noting that the somatic cell hybrids of the American mink-domestic pig type (Sus scrofa f. domestica L., 1758) are successfully used for cytogenetic studies in the latter species (ZHDANOVA et al, 1996;KOROLEVA et al, 1998).…”

“…Genetic studies related to the economic use of the American mink focus on the inheritance of coat color (SHACKELFORD, 1948;TRAPEZOV, 1997;BENKEL et al, 2009), chromosome banding (MANDAHL and FREDGA, 1975), location of genes by comparative cytogenetics and somatic hybridization methods (PACK et al, 1992;HAMEISTER et al, 1997;GRAPHODATSKY et al, 2000;KUZNETSOV et al, 2003), identification of microsatellite markers (ANISTOROAEI et al, 2006;ANSARI et al, 2007), construction of genetic maps (linkage maps) and physical maps (ANISTOROAEI et al, 2007;ANISTOROAEI et al, 2009;BENKEL et al, 2012), mapping of single nucleotide polymorphisms (SNPs) (BENKEL et al, 2008;SKORUPSKI, 2009;SKORUPSKI and KMIEĆ 2011;KMIEĆ et al, 2013a and identification of quantitative trait loci (QTLs) (THIRSTRUP et al, 2012).…”