Characterization of a new endothelial cell growth factor (f-ECGF) partially purified from the supernatant of human fibroblast cells.

Satoh, Takeshi; Kan, Mikio; Masuko, Kazue; Obinata, Masuo

doi:10.1247/csf.14.731

Cited by 8 publications

(6 citation statements)

References 25 publications

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“…Some of these cells stained for alpha-smooth muscle actin and, when isolated, showed morphological features of myofibroblasts. Consistent with the observations that expression of TSP is enhanced by fibroblasts and smooth muscle cell mitogens (10,12), that antibodies against TSP inhibit growth of rat aortic smooth muscle ceils (14), and that TSP stimulates the growth of fibroblasts ( growth factor(s) as previously described (32,33). Myofibroblasts may promote angiogenesis also by producing extracellular matrix molecules required by the endothelial cells (32).…”

Section: Discussionsupporting

confidence: 85%

Matrix-bound thrombospondin promotes angiogenesis in vitro

Nicosia

Tuszynski

1994

The Journal of Cell Biology

159

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Abstract. Thrombospondin (TSP) is a multidomain adhesive protein postulated to play an important role in the biological activity of the extracellular matrix. To test this hypothesis, TSP-containing fibrin and collagen matrices were evaluated for their capacity to support angiogenesis and cell growth from explants of rat aorta. This serum-free model allowed us to study the angiogenic effect of TSP without the interference of attachment and growth factors present in serum. TSP promoted dose-dependent growth of microvessels and fibroblast-like cells. The number of microvessels in TSP-containing collagen and fibrin gels increased by 136 and 94%, respectively. The TSP effect was due in part to cell proliferation since a 97% increase in [3H]thymidine incorporation by the aortic culture was observed. The effect was TSP-specific because TSP preparations adsorbed with anti-TSP antibody showed no activity. TSP did not promote angiogenesis directly since no TSP-dependent growth of isolated endothelial cells could be demonstrated. Rather TSP directly stimulated the growth of aortic culture-derived myofibroblasts which in turn promoted microvessel formation when cocultured with the aortic explants. Angiogenesis was also stimulated by myofibroblastconditioned medium. Partial characterization of the conditioned medium suggests that the angiogenic activity is due to heparin-binding protein(s) with molecular weight >30 kD. These results indicate that matrixbound TSP can indirectly promote microvessel formation through growth-promoting effects on myofibroblasts and that TSP may be an important stimulator of angiogenesis and wound healing in vivo.

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Section: Discussionsupporting

confidence: 85%

Matrix-bound thrombospondin promotes angiogenesis in vitro

Nicosia

Tuszynski

1994

The Journal of Cell Biology

159

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“…It therefore follows that rapid cell migration onto the luminal surface of the graft would be advantageous in preventing graft occlusion. 19,37,40 The velocity of cell migration is dependent on the structure of the luminal surface and biocompatibility of the synthetic material. 26,37,40,41 In this study, we demonstrated that the velocity of neointimal cell migration onto the Data presented as percentages.…”

Section: Discussionmentioning

confidence: 99%

“…19,37,40 The velocity of cell migration is dependent on the structure of the luminal surface and biocompatibility of the synthetic material. 26,37,40,41 In this study, we demonstrated that the velocity of neointimal cell migration onto the Data presented as percentages.…”

Section: Discussionmentioning

confidence: 99%

Polyurethane vascular prostheses decreases neointimal formation compared with expanded polytetrafluoroethylene

Jeschke¹,

Hermanutz²,

Wolf³

et al. 1999

Journal of Vascular Surgery

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“…Acidic fibroblast growth factor has also been known to stimulate proliferation and migration of vascular endothelial cells (Baudinet al 1990;Reygrobellet et al 1991). It has been already shown that endothelial cell growth factor (ECGF) stimulates severe neovascularization when applied into the rabbit cornea (Satoh et al 1989).…”

Section: Discussionmentioning

confidence: 99%

Effect of Octreotide on experimental corneal neovascularization

Demır¹,

Çeliker²,

Kükner³

et al. 1999

Acta Ophthalmologica Scandinavica

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ABSTRACT.Purpose:To examine the ability of subcutaneously administered Octreotide ( a long acting somatostatin analoque) to serve as an inhibitory agent for corneal neovascularization in eyes of Wistar Albino rats. Methods: Neovascular growth into the corneas of all the animals was induced by silver nitrate cauterization. Half of the animals which were randomly selected for the Octreotide group received 30 micrograms systemic Octreotide for 7 days. The treatment was initiated on the same day as chemical cauterization. The rest of the animals (control group) received no treatment. Slit lamp and histopathologic examination of the corneas of both groups were performed at the end of the study period. Results: It was observed that the corneal neovascularization and histopatologic scores of the Octreotide group were significantly lower than those of the control group (p∞0.001, p∞0.01). Conclusion: Systemic administration of Octreotide inhibits the corneal neovascular response in a rat model.

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Characterization of a new endothelial cell growth factor (f-ECGF) partially purified from the supernatant of human fibroblast cells.

Cited by 8 publications

References 25 publications

Matrix-bound thrombospondin promotes angiogenesis in vitro

Matrix-bound thrombospondin promotes angiogenesis in vitro

Polyurethane vascular prostheses decreases neointimal formation compared with expanded polytetrafluoroethylene

Effect of Octreotide on experimental corneal neovascularization

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