Characterization of a new duplicate δ-opioid receptor from zebrafish

Pinal, Noelia; Martin, Ivan Rodríguez; González-Núñez, Verónica; Velasco, Ezequiel Marrón Fernández de; Alvarez, Francisco Alvar; González-Sarmiento, Rogelio; Rodrı́guez, Raquel E.

doi:10.1677/jme.1.02136

Cited by 50 publications

(36 citation statements)

References 53 publications

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“…1). Zebrafish has an extra copy of the delta receptor gene (20) and medaka has duplicates of both the delta and kappa receptor genes. The extra copies are likely to be a result of a third whole-genome duplication (3R) that took place early in ray-finned fish evolution.…”

Section: Discussionmentioning

confidence: 99%

“…The genes arose by duplications in the common ancestor of tetrapods and bony fishes (13). Opioid receptor sequences have been reported for a few nonmammalian tetrapods (14)(15)(16)(17)(18)) and a few teleost fishes (19)(20)(21)(22)(23)(24), and a partial sequence has been reported for a hagfish (19). Functional studies in amphibians and bony fishes have shown that the opioid system is involved in nociception also in these species (25,26).…”

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confidence: 99%

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Evolution of vertebrate opioid receptors

Dreborg

Sundström

Larsson

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

110

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The opioid peptides and receptors have prominent roles in pain transmission and reward mechanisms in mammals. The evolution of the opioid receptors has so far been little studied, with only a few reports on species other than tetrapods. We have investigated species representing a broader range of vertebrates and found that the four opioid receptor types (delta, kappa, mu, and NOP) are present in most of the species. The gene relationships were deduced by using both phylogenetic analyses and chromosomal location relative to 20 neighboring gene families in databases of assembled genomes. The combined results show that the vertebrate opioid receptor gene family arose by quadruplication of a large chromosomal block containing at least 14 other gene families. The quadruplication seems to coincide with, and, therefore, probably resulted from, the two proposed genome duplications in early vertebrate evolution. We conclude that the quartet of opioid receptors was already present at the origin of jawed vertebrates Ϸ450 million years ago. A few additional opioid receptor gene duplications have occurred in bony fishes. Interestingly, the ancestral receptor gene duplications coincide with the origin of the four opioid peptide precursor genes. Thus, the complete vertebrate opioid system was already established in the first jawed vertebrates.chromosome ͉ G protein-coupled receptor ͉ gene duplication S everal opioid peptides, including endorphin and enkephalins, are important regulators of nociceptive neurotransmission and reward mechanisms in mammals. Specific binding sites in the brain for opioid compounds were first reported in 1973 (1-3), and it was soon evident that more than one type of binding site existed (4). Subsequently three distinct opioid receptors were identified and designated delta, kappa, and mu. These receptors were cloned and found to be encoded by separate genes belonging to the superfamily of rhodopsin-like G protein-coupled receptors (GPCRs) (5-8). The genes for the opioid receptors (OPR) have been named OPRD1 (delta), OPRK1 (kappa), and OPRM1 (mu) by the HUGO Gene Nomenclature Committee (HGNC).Homology searches resulted in the discovery of a fourth receptor in both rodents and humans initially named ORL1 for opioid receptor-like (9) or LC132 (10). This receptor shows 48-49% identity to the other three human receptors, which display 55-58% identity among one another. The receptor has been named NOP by the International Union of Basic and Clinical Pharmacology and its gene has been named OPRL1 by HGNC. An endogenous peptide ligand with some similarity to the other opioid peptides was discovered and named nociceptin (11) or orphanin FQ (12).The evolution of the endogenous opioid peptide ligands has been studied extensively and the major peptide ligands are generated from four prepropeptides that are encoded by separate genes in tetrapods. The genes arose by duplications in the common ancestor of tetrapods and bony fishes (13). Opioid receptor sequences have been reported for a few nonmammalian tetrapods (1...

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Evolution of vertebrate opioid receptors

Dreborg

Sundström

Larsson

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

110

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“…The study of the direct effects of morphine in the embryos will provide a better understanding on the molecular mechanisms that underlie the physical and neurobehavioral defects shown in fetuses and offspring after maternal morphine consumption (Nasiraei-Moghadam et al, 2010). In addition, the endogenous opioid system has been characterized in the zebrafish, which has a MOR (zfMOR), two DOR duplicates (zfDOR1 and zfDOR2), a -opioid receptor, and an opioid receptor-like Rodriguez et al, 2000;Alvarez et al, 2006;Pinal-Seoane et al, 2006). The opioid-induced drug addiction pathway has been suggested to involve the midbrain dopaminergic neurons lo-cated within ventral tegmental area and the nucleus accumbens (NAc).…”

Section: Introductionmentioning

confidence: 99%

Morphine Regulates Dopaminergic Neuron Differentiation via miR-133b

Sánchez-Simón

Zhang

Loh³

et al. 2010

Mol Pharmacol

101

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Morphine is one of the analgesics used most to treat chronic pain, although its long-term administration produces tolerance and dependence through neuronal plasticity. The ability of morphine to regulate neuron differentiation in vivo has been reported. However, the detailed mechanisms have not yet been elucidated because of the inability to separate maternal influences from embryonic events. Using zebrafish embryos as the model, we demonstrate that morphine decreases miR-133b expression, hence increasing the expression of its target, Pitx3, a transcription factor that activates tyrosine hydroxylase and dopamine transporter. Using a specific morpholino to knock down the zebrafish -opioid receptor (zfMOR) in the embryos and selective mitogen-activated protein kinase inhibitors, we demonstrate that the morphine-induced miR-133b decrease in zebrafish embryos is mediated by zfMOR activation of extracellular signal-regulated kinase 1/2. A parallel morphine-induced down-regulation of miR-133b was observed in the immature but not in mature rat hippocampal neurons. Our results indicate for the first time that zebrafish embryos express a functional -opioid receptor and that zebrafish serves as an excellent model to investigate the roles of microRNA in neuronal development affected by long-term morphine exposure.

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“…To clone the drNOP gene, we have followed the standard methodology previously described (Barrallo et al 1998, Pinal-Seoane et al 2006. Oligonucleotides were designed using the Oligo 4.05 Primer Analysis Software (National Biosciences, Inc., Plymouth, MN, USA), DNA sequences were analyzed with Chromas 2.3 (School of Health Science, Griffith University, Australia) software and compared with other nucleotide and/or protein sequence databases using the FASTA or BLAST programs from EMBL and from the NCBI websites (http://www.ebi.ac.uk/embl/ and http://www.ncbi.…”

Section: Cloning Sequence and Phylogenetic Analysismentioning

confidence: 99%

Pharmacological characterization of a nociceptin receptor from zebrafish (Danio rerio)

Rivas-Boyero

Herrero-Turrión²,

González-Núñez³

et al. 2011

Journal of Molecular Endocrinology

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The nociceptin receptor (NOP) and its endogenous ligand, nociceptin/orphanin FQ (OFQ), are involved in a wide range of biological functions, such as pain, anxiety, learning, and memory. The zebrafish has been proposed as a candidate to study the in vivo effects of several drugs of abuse and to discover new pharmacological targets. We report the cloning, expression, and pharmacological characterization of a NOP receptor from zebrafish (drNOP). The full-length cDNA codes a protein of 363 residues, which shows high sequence similarity to other NOPs. Phylogenetic analysis indicates that NOPs are broadly conserved during vertebrate evolution, and that they stand for the most divergent clade of the opioid/OFQ receptor family. Expression studies have revealed that drNOP mRNA is highly expressed in the central nervous system, and low expression levels are also found in peripheral tissues such as gills, muscle, and liver. Pharmacological analysis indicates that drNOP displays specific and saturable binding for 4, H]nociceptin, with a K d Z0 . 20G0 . 02 nM and a B max Z1703G81 fmol/mg protein. 35 S] GTPgS stimulation studies showed that drNOP receptor is functional, as nociceptin is able to fully activate the receptor and DYN A behaves as a partial agonist (50% stimulation). Our results indicate that drNOP receptor displays mixed characteristics of both NOP and k opioid receptors. Hence, drNOP, which has retained more of the likely ancestral features, bridges the gap between nociceptin and opiate pharmacology.

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Characterization of a new duplicate δ-opioid receptor from zebrafish

Cited by 50 publications

References 53 publications

Evolution of vertebrate opioid receptors

Evolution of vertebrate opioid receptors

Morphine Regulates Dopaminergic Neuron Differentiation via miR-133b

Pharmacological characterization of a nociceptin receptor from zebrafish (Danio rerio)

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