Characterization of a new antifungal non-specific lipid transfer protein (nsLTP) from sugar beet leaves

Kristensen, Anne K.; Brunstedt, Janne; Nielsen, Kristian; Roepstorff, Peter; Mikkelsen, Jørn Dalgaard

doi:10.1016/s0168-9452(00)00190-4

Cited by 53 publications

(30 citation statements)

References 38 publications

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“…The expression pattern of nsLTPs from different plants has shown a large divergence covering various tissues and against environmental stimulates (George and Parida 2010;Kader 1996;Pitzschke et al 2013;Pyee et al 1994). Consistent with their complex expression profiles, the pleiotropic functions of nsLTPs have been previously suggested to be involved in cuticle synthesis (Lee et al 2009;Pyee et al 1994), catabolism in lipid storage (Tsuboi et al 1992), somatic embryogenesis (Kader 1996), stigmapollen interaction (Huang et al 2013;Mollet et al 2000;Tian et al 2013), defense signaling (Blein et al 2002;Maldonado et al 2002), nodule organogenesis (Lei et al 2014) development, and antimicrobial activities (Caaveiro et al 1997;Kristensen et al 2000). However, the exact function of nsLTPs remains to be clearly established.…”

Section: Introductionmentioning

confidence: 96%

“…Recent research data demonstrated that the disruption of a nsLTP-like protein (LTPG1) simultaneously altered the cuticular lipid composition and enhanced the susceptibility to the fungal pathogen, Alternaria brasssicola, supporting the overlapping involvement of the nsLTP and cuticle in defense mechanism (Lee et al 2009). However, it is unclear whether the susceptibility to the pathogens of LTPknockout plants is attributed to cuticular lipid changes or its antimicrobial activities (Kristensen et al 2000). An alternative explanation of nsLTPs involved in the defense against fungal pathogens is that the cutin monomer-nsLTP complex is released by the fungal cutinase at the time of infection, which can act as signaling molecules and bind to the plasmalemma receptor triggering plant defense responses (Blein et al 2002).…”

Section: Introductionmentioning

confidence: 99%

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Identification of wheat non-specific lipid transfer proteins involved in chilling tolerance

Hou

et al. 2014

Plant Cell Rep

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Three TaLTPs were found to enhance chilling tolerance of transgenic Arabidopsis, which were characterized by analyzes of promoter-GUS activity, subcellular localization, chromosomal location and transcriptional profile. Non-specific lipid transfer proteins (nsLTP) are abundantly expressed in plants, however, their functions are still unclear. In this study, we primarily characterized the functions of 3 type I TaLTP genes that were localized on chromosomes 3A, 3B, and 5D, respectively. The transcripts of TaLTPIb.1 and TaLTPIb.5 were induced under chilling, wound, and drought conditions, while TaLTPId.1 was only up-regulated by dark treatment. All the 3 TaLTP genes could be stimulated by the in vitro treatment of salicylic acid, while TaLTPId.1 was also positively regulated by methyljasmonic acid. Furthermore, the promoter-reporter assay of TaLTPIb.1 in the transgenic brachypodium showed a typical epidermis-specific expression pattern of this gene cluster. When fused with EGFP, all the 3 proteins were shown to localize on the plasma membrane in transgenic tobacco, although a signal in chloroplasts was also observed for TaLTPId.1. Heterogeneous overexpression of each of the TaLTP genes in Arabidopsis resulted in longer root length compared with wild type plants under chilling condition. These results suggest that type I TaLTPs may have a conserved functionality in chilling tolerance by lipid permeation in the plasma membrane of epidermal cells. On the other hand, the type I TaLTPs may exert functional divergence mainly through regulatory subfunctionalization.

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Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Identification of wheat non-specific lipid transfer proteins involved in chilling tolerance

Hou

et al. 2014

Plant Cell Rep

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“…They are involved in plant defense, with strong evidence supporting their mutual involvement in cuticle deposition. LTPs are induced by pathogens (Molina and García-Olmedo, 1993;Guiderdoni et al, 2002;Park et al, 2002), display antimicrobial activity (Nielsen et al, 1996;Molina and Garcia-Olmedo, 1997;Kristensen et al, 2000), and are involved in systemic resistance signaling (Maldonado et al, 2002). Furthermore, many LTP genes are induced by drought stress and/or water deficit (ColmeneroFlores et al, 1997;Jang et al, 2004), and some are up-regulated when exposed to both pathogens and dehydration stress (Jung et al, 2003).…”

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confidence: 99%

Increased Accumulation of Cuticular Wax and Expression of Lipid Transfer Protein in Response to Periodic Drying Events in Leaves of Tree Tobacco

2005

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Cuticular wax deposition and composition affects drought tolerance and yield in plants. We examined the relationship between wax and dehydration stress by characterizing the leaf cuticular wax of tree tobacco (Nicotiana glauca L. Graham) grown under periodic dehydration stress. Total leaf cuticular wax load increased after each of three periods of dehydration stress using a CH 2 Cl 2 extraction process. Overall, total wax load increased 1.5-to 2.5-fold, but composition of the wax was not altered. Homologous series of wax components were classified into organic groups; n-hentriacontane was the largest component (.75%) with alcohols and fatty acids representing ,10% of the entire wax load. An increase in density, but no change in the three-dimensional shape, of leaf wax crystals was evident under low-kV scanning electron microscopy after each drying event. Leaves excised from plants subjected to multiple drying events were more resistant to water loss compared to leaves excised from well-watered plants, indicating that there is a negative relationship between total wax load and epidermal conductance. Lipid transfer proteins (LTPs) are thought to be involved in the transfer of lipids through the extracellular matrix for the formation of cuticular wax. Using northern analysis, a 6-fold increase of tree tobacco LTP gene transcripts was observed after three drying events, providing further evidence that LTP is involved in cuticle deposition. The simplicity of wax composition and the dramatic wax bloom displayed by tree tobacco make this an excellent species in which to study the relationship between leaf wax deposition and drought tolerance.

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“…Several nsLTP genes in barley are also up-regulated in response to infection by various strains of fungal pathogens (Garcia-OImedo et al, 1995). Their role in plant defenses has been confirmed by Kristensen et al (2000), who have reported that nsLTPs isolated from the seeds of radish and onion, or the leaves of barley, maize, spinach, and sugar beet, exhibit antipathogenic activity in vitro.…”

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confidence: 79%

Cloning, sequence analysis, and prokaryotic expression of cDNA encoding a putative non-specific lipid-transfer protein from the bracts of dovetree (Davidia involucrata Baill)

2003

J. Plant Biol.

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The 967-bp clone PIA5 was isolated from a suppression subtractive hybridization cDNA library of dovetree bracts (Davidia involucrata Baill.). A complete cDNA of 1047 bp was obtained via 5'.RACE (5'-Rapid Amplification of cDNA End) techniques, using the gene-specific primer PIAS.1. Northern blot analyses showed that the gene was predominantly expressed in the bracts, while the blotting signal from the leaves was weak. Its deduced amino acid sequence was most highly homologous to the lipid-transfer protein 3 precursor isolated from upland cotton, the lipid-transfer protein SDi.9 from the common sunflower, and the non-specific lipid-transfer protein precursor allergen from sweet cherry. It also had features in common with plant nsLTPs (non-specific lipid-transfer proteins), including eight conserved cysteine residues, a high isoelectric point (8.9), and a lack of tryptophans. The deduced amino acid sequence had two transmembrane helices --the first from Position 5 (Gly) to Position 35 (Val); the second, from Position 28 (Ala) to Position 46 (Leu). A cleavage site for the putative signal peptide was predicted to occur between Positions 28 (Ala) and 29 (Ala). Therefore, the putative mature form of the protein would comprise 92 amino acids, with a molecular weight of 9.2 kD. All these results provide compelling evidence that the PIA5 clone belongs to the nsLTP1 gene family, thus being named the PIA5 putative nsLTP1 gene. This is the first nsLTP gene reported from Davidiaceae.

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Characterization of a new antifungal non-specific lipid transfer protein (nsLTP) from sugar beet leaves

Cited by 53 publications

References 38 publications

Identification of wheat non-specific lipid transfer proteins involved in chilling tolerance

Identification of wheat non-specific lipid transfer proteins involved in chilling tolerance

Increased Accumulation of Cuticular Wax and Expression of Lipid Transfer Protein in Response to Periodic Drying Events in Leaves of Tree Tobacco

Cloning, sequence analysis, and prokaryotic expression of cDNA encoding a putative non-specific lipid-transfer protein from the bracts of dovetree (Davidia involucrata Baill)

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