Characterization of a Neutrophil Cell Surface Glycosaminoglycan That Mediates Binding of Platelet Factor 4

Abstract

Platelet factor 4 (PF-4) is a platelet-derived

Immunological Activity of Chondroitin Sulfate

“…Our laboratory has also reported the many biological activities of the chemically fully sulfated poly-and oligosaccharides (Chaidedgumjorn et al, 2002;Suzuki et al, 2001;Toida et al, 1999Toida et al, , 2000. Chondroitin sulfate has been found in many tissues (Suzuki et al, 1968) and cells (Ohhashi et al, 1984;Petersen et al, 1999;Stevens et al, 1988), and has been reported to interact with various biologically important molecules and regulate their functions. We have demonstrated the importance of the content, position, and number of O-sulfo groups in CS for immunological activity of the OVA-stimulated murine splenocytes in vitro (Akiyama et al, 2004;Sakai et al, 2002a).…”

Platelet Factor 4/CXCL4 Induces Phagocytosis and the Generation of Reactive Oxygen Metabolites in Mononuclear Phagocytes Independently of Gi Protein Activation or Intracellular Calcium Transients

“…However, this molecule was expressed neither on monocytes nor on neutrophils. We have shown previously that on human neutrophils a chondroitin sulfate proteoglycan specifically binds PF-4 and is involved in cell activation (14,42), indicating that there may exist more than one receptor for the chemokine. The hypothesis of PF-4-binding to such a type of receptor is supported by our unpublished observation that heparin completely abolished PF-4-induced monocyte oxidative burst.…”

Structural Requirements of Heparan Sulfate for the Binding to the Tumor-derived Adhesion Factor/Angiomodulin That Induces Cord-like Structures to ECV-304 Human Carcinoma Cells

“…A little yet appreciable amount of the disaccharide unit ⌬HexA-GalNAc(4,6-O-disulfate) typical for CS-E was found, representing 2-3% of the total CS disaccharides as shown in Table IV. Recently it has been demonstrated that CS-E binds to various types of collagen to different degrees (24) and platelet factor 4 (27). CS-E is expressed in a cell-type-and tissue-type-specific manner and in a developmentally regulated fashion (reviewed in Ref.…”