Characterization of a Monoclonal Anti-H-2Kb Antibody

Köhler, Georges; Lindahl, Kinten Fischer; Heusser, C H

doi:10.1159/000406088

Cited by 49 publications

(30 citation statements)

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“…Tissues from Zn-treated and from uninduced transgenic mice were analyzed immunohistochemically with B8-24-3 (20). Staining was observed in those tissues that had sMTp-Kb transcripts (Fig.…”

Section: Resultsmentioning

confidence: 99%

Expression in transgenic mice of class I histocompatibility antigens controlled by the metallothionein promoter.

Morahan

Brennan

Bhathal

et al. 1989

Proc. Natl. Acad. Sci. U.S.A.

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To study the effects of increased expression of major histocompatibility complex class I molecules on the development of self-tolerance, transgenic mice were produced that expressed the H-2Kb gene under the control of the metallothionein promoter. Administration of zinc enhanced transgene expression in liver, kidney and exocrine pancreas. No evidence suggestive of an autoimmune response was found in transgene-expressing tissues in mice otherwise allogeneic to H-2K0. Despite this lack of responsiveness in vivo, T cells could be stimulated in vitro to lyse H-2Kb-bearing target cells. No infiltration was detected in transgenic mice after irradiation and reconstitution with bone marrow cells. When spleen cells were used for reconstitution, however, dense lymphocytic infiltration was seen, particularly in the portal tracts of the liver, and this was accompanied by piecemeal necrosis and apoptosis of periportal hepatocytes. This aggressive response progressively diminished with time, and by 12 weeks after reconstitution many of the portal tracts were free of infiltration while the others showed no accompanying necrosis. The picture at this stage was similar to that seen in chronic persistent hepatitis. These results suggest that, in addition to negative selection in the thymus, peripheral mechanisms not involving clonal deletion or permanent clonal anergy can prevent immune responses to self molecules.

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“…Tissues from Zn-treated and from uninduced transgenic mice were analyzed immunohistochemically with B8-24-3 (20). Staining was observed in those tissues that had sMTp-Kb transcripts (Fig.…”

Section: Resultsmentioning

confidence: 99%

Expression in transgenic mice of class I histocompatibility antigens controlled by the metallothionein promoter.

Morahan

Brennan

Bhathal

et al. 1989

Proc. Natl. Acad. Sci. U.S.A.

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“…In standard stabilization assays, cells were incubated at 26°C for 30 min with serial 3-fold dilutions of the peptides in a total volume of 200 l of Dulbecco's modified Eagle's medium, 0.5% bovine serum albumin. The temperature was raised to 37°C for 45 min, and cells were collected and stained with the fluorescein isothiocyanate-labeled monoclonal antibody B8.24.3 (Köhler et al, 1981), which detects conformationally intact H-2K b . The levels of cell-surface expression were analyzed by flow cytometry using a FACScan apparatus (Becton Dickinson, Heidelberg, Germany).…”

Section: Methodsmentioning

confidence: 99%

Tolerance to Amino Acid Variations in Peptides Binding to the Major Histocompatibility Complex Class I Protein H-2Kb

Udaka

Wiesmüller

Kienle

et al. 1995

Journal of Biological Chemistry

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“…Transfectants were stained with the K b -specific mAb B8-24.3 (34), sorted in a Becton Dickinson (Mountain View, CA) FACSort, and subcloned to yield a clonal population of L1210-Fas antisense-K b cells. Subsequently, these cells were transfected either with pEF-PGK puro crmA-flag (35) alone (designated LKC) or with pEF-PGK puro crmA-flag plus pFM92-mCD95L (designated LKC-CD95L).…”

Section: Generation Of Lkc-cd95l and L1210-fas Antisense Expressing Kmentioning

confidence: 99%

CD95 Ligand-Expressing Tumors Are Rejected in Anti-Tumor TCR Transgenic Perforin Knockout Mice

Behrens

Igney

Arnold

et al. 2001

The Journal of Immunology

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CD95 (APO-/Fas) ligand (CD95L) is a member of the TNF family predominantly expressed by activated T and NK cells but also by tumors of diverse cellular origin. CD95L trimerizes surface CD95 expressed by target cells that subsequently undergo apoptosis. The role of the CD95/CD95L system in the down-regulation of an immune response (activation-induced cell death) is established. However, it is so far unclear why tumors express CD95L. To investigate whether tumors use the CD95L to down-regulate an anti-tumor immune response, we established a transgenic (tg) mouse model consisting of 1) apoptosis-resistant tumor cells, designated LKC-CD95L, which express functional CD95L and the model tumor Ag Kb; and 2) perforin knockout (PKO) anti-Kb TCR tg mice. L1210-Fas antisense expressing Kb, crmA, and CD95L (LKC-CD95L) killed CD95+ unrelated tumor targets and Con A-activated splenocytes from anti-Kb TCR tg PKO mice by a CD95L-dependent mechanism in vitro. However, we could not detect any cytotoxic activity against anti-tumor (anti-Kb) T cells in vivo. We also observed reduced growth of LKC-CD95L in nude mice and rapid rejection in anti-Kb TCR tg PKO mice. Because the tumor cells are resistant to CD95L-, TNF-α-, and TNF-related apoptosis-inducing ligand-induced apoptosis and the mice used are perforin-deficient, the involvement of these four cytotoxicity mechanisms in tumor rejection can be excluded. The histological examination of tumors grown in nude mice showed infiltration of LKC-CD95L tumors by neutrophils, whereas L1210-Fas antisense expressing Kb and crmA (LKC) tumor tissue was neutrophil-free. Chemotaxis experiments revealed that CD95L has no direct neutrophil-attractive activity. Therefore, we conclude that LKC-CD95L cells used an indirect mechanism to attract neutrophils that may cause tumor rejection.

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Characterization of a Monoclonal Anti-H-2Kb Antibody

Cited by 49 publications

References 0 publications

Expression in transgenic mice of class I histocompatibility antigens controlled by the metallothionein promoter.

Expression in transgenic mice of class I histocompatibility antigens controlled by the metallothionein promoter.

Tolerance to Amino Acid Variations in Peptides Binding to the Major Histocompatibility Complex Class I Protein H-2Kb

CD95 Ligand-Expressing Tumors Are Rejected in Anti-Tumor TCR Transgenic Perforin Knockout Mice

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