The Immune System
DOI: 10.1159/000406088
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Characterization of a Monoclonal Anti-H-2Kb Antibody

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Cited by 49 publications
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“…Tissues from Zn-treated and from uninduced transgenic mice were analyzed immunohistochemically with B8-24-3 (20). Staining was observed in those tissues that had sMTp-Kb transcripts (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Tissues from Zn-treated and from uninduced transgenic mice were analyzed immunohistochemically with B8-24-3 (20). Staining was observed in those tissues that had sMTp-Kb transcripts (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…In standard stabilization assays, cells were incubated at 26°C for 30 min with serial 3-fold dilutions of the peptides in a total volume of 200 l of Dulbecco's modified Eagle's medium, 0.5% bovine serum albumin. The temperature was raised to 37°C for 45 min, and cells were collected and stained with the fluorescein isothiocyanate-labeled monoclonal antibody B8.24.3 (Köhler et al, 1981), which detects conformationally intact H-2K b . The levels of cell-surface expression were analyzed by flow cytometry using a FACScan apparatus (Becton Dickinson, Heidelberg, Germany).…”
Section: Methodsmentioning
confidence: 99%
“…Transfectants were stained with the K b -specific mAb B8-24.3 (34), sorted in a Becton Dickinson (Mountain View, CA) FACSort, and subcloned to yield a clonal population of L1210-Fas antisense-K b cells. Subsequently, these cells were transfected either with pEF-PGK puro crmA-flag (35) alone (designated LKC) or with pEF-PGK puro crmA-flag plus pFM92-mCD95L (designated LKC-CD95L).…”
Section: Generation Of Lkc-cd95l and L1210-fas Antisense Expressing Kmentioning
confidence: 99%