Characterization of a maize heat-shock protein 101 gene, HSP101, encoding a ClpB/Hsp100 protein homologue

Nieto-Sotelo, J.; Kannan, Kavitha; Martínez, Luz María; Segal, Claudia

doi:10.1016/s0378-1119(99)00060-8

Cited by 51 publications

(41 citation statements)

References 18 publications

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“…These include ABA/stressinducible proteins, heat shock proteins, late embryogenesis abundant (LEA) proteins, and proteases (Yacoob and Filion, 1986;Prasad, 1996;Nieto-Sotelo et al, 1999;White et al, 2000). Upon comparing the accession numbers of drought and cold-induced genes in Arabidopsis reported by Seki et al (2001) with the differentially expressed genes in this study (Tables I-IV), we found none of the genes to be in common.…”

Section: Differentially Expressed Genesmentioning

confidence: 69%

Expression Profiling of Reciprocal Maize Hybrids Divergent for Cold Germination and Desiccation Tolerance

et al. 2002

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Recombinant inbred lines (RILs) derived from B73 ϫ M017 were screened for cold germination (CG) and desiccation tolerance (DT) phenotypes. Reciprocal F 1 hybrids were made between divergent RILs, and hybrids that showed differential phenotypes (parent-of-origin effect) for CG or DT were selected for profiling mRNA and protein expression. mRNA and proteins were extracted from embryo axes of seed germinated for 11 d at 12.5°C in the dark and developing embryos at 40% seed moisture (R5 stage) for CG and DT, respectively. GeneCalling analysis, an open-ended mRNA profiling method, identified 336 of 32,496 and 656 of 32,940 cDNA fragments that showed Ն1.5-fold change in expression between the reciprocal F 1 hybrids for CG and DT, respectively. Protein expression map (PEM) analysis, an open-ended two-dimensional polyacrylamide gel electrophoresis, identified 117 of 2,641 and 205 of 1,876 detected proteins to be differentially expressed with Ն1.5-fold change between the reciprocal F 1 hybrids in CG and DT samples, respectively. A subset of these proteins was identified by tandem mass spectrometry followed by database query of the spectra. The differentially expressed genes/ proteins were classified into various functional groups including carbohydrate and amino acid metabolism, ion transporters, stress and defense response, polyamine metabolism, chaperonins, cytoskeleton associated, etc. Phenotypic analysis of seed from self-pollinated ears of the reciprocal F 1 hybrids displayed small differences compared with the reciprocal hybrids themselves, suggesting a negligible effect of cytoplasmic factors on CG and DT traits. The results provide leads to improving our understanding of the genes involved in stress response during seed maturation and germination.

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Section: Differentially Expressed Genesmentioning

confidence: 69%

Expression Profiling of Reciprocal Maize Hybrids Divergent for Cold Germination and Desiccation Tolerance

et al. 2002

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“…Members of the class I subfamilies are divided by the length of their middle region. The middle region in A, B, and C subfamily members is predicted to form a coiled-coil supersecondary structure (Celerin et al, 1998;Nieto-Sotelo et al, 1999). Both the NBD and the middle region are important for function (Schirmer et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Maize HSP101 Plays Important Roles in Both Induced and Basal Thermotolerance and Primary Root Growth

et al. 2002

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HSP101 belongs to the ClpB protein subfamily whose members promote the renaturation of protein aggregates and are essential for the induction of thermotolerance. We found that maize HSP101 accumulated in mature kernels in the absence of heat stress. At optimal temperatures, HSP101 disappeared within the first 3 days after imbibition, although its levels increased in response to heat shock. In embryonic cells, HSP101 concentrated in the nucleus and in some nucleoli. Hsp101 maps near the umc132 and npi280 markers on chromosome 6. Five maize hsp101-m-:: Mu1 alleles were isolated. Mutants were null for HSP101 and defective in both induced and basal thermotolerance. Moreover, during the first 3 days after imbibition, primary roots grew faster in the mutants at optimal temperature. Thus, HSP101 is a nucleus-localized protein that, in addition to its role in thermotolerance, negatively influences the growth rate of the primary root. HSP101 is dispensable for proper embryo and whole plant development in the absence of heat stress.

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“…Genetic evidence has established that the Hsp100 family proteins are essential for the acquisition of thermotolerance in plants. Loss-of-function mutants of Hsp101 in Arabidopsis (Arabidopsis thaliana; hot1; Vierling, 2000, 2001) and maize (Zea mays; Nieto-Sotelo et al, 1999) are unable to acquire thermotolerance at several different growth stages.…”

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confidence: 99%

Heat Stress Phenotypes of Arabidopsis Mutants Implicate Multiple Signaling Pathways in the Acquisition of Thermotolerance

et al. 2005

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To investigate the importance of different processes to heat stress tolerance, 45 Arabidopsis (Arabidopsis thaliana) mutants and one transgenic line were tested for basal and acquired thermotolerance at different stages of growth. Plants tested were defective in signaling pathways (abscisic acid, salicylic acid, ethylene, and oxidative burst signaling) and in reactive oxygen metabolism (ascorbic acid or glutathione production, catalase) or had previously been found to have temperature-related phenotypes (e.g. fatty acid desaturase mutants, uvh6). Mutants were assessed for thermotolerance defects in seed germination, hypocotyl elongation, root growth, and seedling survival. To assess oxidative damage and alterations in the heat shock response, thiobarbituric acid reactive substances, heat shock protein 101, and small heat shock protein levels were determined. Fifteen mutants showed significant phenotypes. Abscisic acid (ABA) signaling mutants (abi1 and abi2) and the UV-sensitive mutant, uvh6, showed the strongest defects in acquired thermotolerance of root growth and seedling survival. Mutations in nicotinamide adenine dinucleotide phosphate oxidase homolog genes (atrbohB and D), ABA biosynthesis mutants (aba1, aba2, and aba3), and NahG transgenic lines (salicylic acid deficient) showed weaker defects. Ethylene signaling mutants (ein2 and etr1) and reactive oxygen metabolism mutants (vtc1, vtc2, npq1, and cad2) were more defective in basal than acquired thermotolerance, especially under high light. All mutants accumulated wild-type levels of heat shock protein 101 and small heat shock proteins. These data indicate that, separate from heat shock protein induction, ABA, active oxygen species, and salicylic acid pathways are involved in acquired thermotolerance and that UVH6 plays a significant role in temperature responses in addition to its role in UV stress.Plants and other organisms have both an inherent ability to survive exposure to temperatures above the optimal for growth (basal thermotolerance) and an ability to acquire tolerance to otherwise lethal heat stress (acquired thermotolerance). Acquired thermotolerance is induced by a short acclimation period at moderately high (but survivable) temperatures or by treatment with other nonlethal stress prior to heat stress (Kapoor et al., 1990;Vierling, 1991;Flahaut et al., 1996;Burke et al., 2000;Hong and Vierling, 2000;Massie et al., 2003;Larkindale et al., 2005). The ability to withstand and to acclimate to supra-optimal temperatures results from both prevention of heat damage and repair of heat-sensitive components. Organisms must also maintain metabolic homeostasis during stress or be able to reestablish homeostasis subsequent to the stress period. Although plants are frequently subjected to dramatic heating to above the optimal growth temperature, relatively little is known about the critical genes controlling either basal or acquired thermotolerance in plants.Heat stress has a complex impact on cell function, suggesting that many processes are involved in th...

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Characterization of a maize heat-shock protein 101 gene, HSP101, encoding a ClpB/Hsp100 protein homologue

Cited by 51 publications

References 18 publications

Expression Profiling of Reciprocal Maize Hybrids Divergent for Cold Germination and Desiccation Tolerance

Expression Profiling of Reciprocal Maize Hybrids Divergent for Cold Germination and Desiccation Tolerance

Maize HSP101 Plays Important Roles in Both Induced and Basal Thermotolerance and Primary Root Growth

Heat Stress Phenotypes of Arabidopsis Mutants Implicate Multiple Signaling Pathways in the Acquisition of Thermotolerance

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