Characterization of a Korean Domestic Cyanobacterium Limnothrix sp. KNUA012 for Biofuel Feedstock

Hong, Ji Won; Jo, Seung-Woo; Kim, Oh Hong; Jeong, Mi Rang; Kim, Hyeon; Park, Kyung Mok; Lee, Kyoung In; Yoon, Ho-Sung

doi:10.5352/jls.2016.26.4.460

Cited by 5 publications

(14 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For instance, hexanol, which has received commercial interest, was found in S. obliquus and C. vulgaris microalgae (44)(45)(46)(47). Other studies have also identified fatty acid components in various microalgae (48), such as n-hexadecanoic acid and octadecanoic acid in a C. sorokiniana extract (49). Results of other studies also revealed that the profile of the fatty acid composition in Chlorella and Scenedesmus extracts is mostly composed of C16-C18 (>92%) (31,47).…”

Section: Resultsmentioning

confidence: 99%

GC-MS profiling, anti-oxidant and anti-diabetic assessments of extracts from microalgae Scenedesmus falcatus (KU.B1) and Chlorella sorokiniana (KU.B2)

et al. 2022

View full text Add to dashboard Cite

Microalgae are a potentially valuable source in the food, pharmaceutical and nutraceutical sectors. While biological activities surveys have investigated the pharmaceutical properties of a few microalgae species, there are not many reports covering biological activity studies. This study was carried out to identify the metabolites by gas chromatography-mass spectrometry and evaluate the anti-oxidant, anti-diabetic properties of green algae extracts, Chlorella sorokiniana (KU.B2) and Scenedesmus falcatus (KU.B1). A total of 51 different chemical constituents were detected and tentatively identified. The primary compounds in both microalgae extracts included (R)-2-hexanol (38.67% in C. sorokiniana and 23.53% in S. falcatus), n-hexadecanoic acid (13.58% in C. sorokiniana and 18.94% in S. falcatus) and octadecanoic acid (22.30% in C. sorokiniana and 32.67% in S. falcatus). According to the profiling results, the C. sorokiniana extract exhibited greater anti-oxidant activity, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging (IC50 = 480.30 ±?14.85 µg ml-1), nitric oxide (NO) radical scavenging (562.73 ±?3.52 µg mL-1) and ferric reducing anti-oxidant power (FRAP) of 58.51 ± 2.42 mgTE g-1. Comparatively, the C. sorokiniana extract had higher contents of alpha-glucosidase and alpha-amylase (IC50 = 491.22 ± 78.41 and 2,817.00 ±143.04 µg mL-1, respectively) than the S. falcatus extract. This first report demonstrated anti-diabetic effect of both extracts on diabetic enzymes. The results confirm microalgae's anti-oxidant and anti-diabetic properties and suggest their potential benefits in cosmeceutical, nutraceutical and pharmaceutical applications.

show abstract

Section: Resultsmentioning

confidence: 99%

GC-MS profiling, anti-oxidant and anti-diabetic assessments of extracts from microalgae Scenedesmus falcatus (KU.B1) and Chlorella sorokiniana (KU.B2)

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Previous research has described saturated fatty acids (SFAs) as biological resources for biofuel production ( Chang et al., 2013 ; Yong et al., 2019 ). These fatty acid components are commonly found in microalgae ( Hong et al., 2016 ), which may be used as a potential source for these valuable compounds ( Ötleş and Pire, 2001 ; Alberts, 2003 ; Herrero et al., 2006 ; Spolaore et al., 2006 ; Knothe, 2008 ; Mohammady, 2011 ). The fatty acid composition of microalgae grown in this study varied with the temperature conditions used; under higher temperatures, SFA concentration was higher.…”

Section: Resultsmentioning

confidence: 99%

“…A 10-day-old culture of each strain originating from aseptically-transferred, isolated colonies (15 mL) (diluted to OD 680 ~0.3, corresponding to the following cell densities: KNUA104, 391.43 ± 16.05 cells × 10 4 ml −1 ; KNUA114, 486.32 ± 29.51 cells × 10 4 ml −1 ; and KNUA122, 523.41 ± 21.73 cells × 10 4 ml −1 ) was inoculated into flasks containing 150 mL of BG-11 medium (performed in triplicates). Flasks were then incubated for 14 days on orbital shakers (VS-202D, Vision Scientific) at 160 rpm in rooms illuminated by fluorescent lamps (300 μmol photons m −2 s −1 ) under controlled temperature (10 °C, 25 °C, or 35 °C) and a light:dark cycle of 16:8 h. The growth of each strain was subsequently measured as previously described ( Hong et al., 2016 ). Algal density was estimated by measuring the OD 680 values with an Optimizer 2120 UV spectrophotometer (Mecasys, South Korea) ( Griffiths et al., 2011 ).…”

Section: Methodsmentioning

confidence: 99%

“…Each microalgal strain was grown for 10 days and the dilutions of all samples (OD 680 = 1.0) were freeze-dried. Thirty milligrams of pulverized, freeze-dried algae were subjected to lipid extraction using a mixture of chloroform and methanol (1:1) as previously described ( Hong et al., 2016 ). Chloroform was evaporated from the extracted mixture using a rotary evaporator.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of Chlorella sorokiniana and Chlorella vulgaris fatty acid components under a wide range of light intensity and growth temperature for their use as biological resources

Yun

Kim

Yoon

2020

Heliyon

Self Cite

View full text Add to dashboard Cite

This study aims to characterize the potential of three strains of microalgal species ( Chlorella sorokiniana KNUA114 and KNUA122; C. vulgaris KNUA104) for use as feedstock, based on their fatty acid compositions. Each strain was molecularly identified using four marker genes ( ITS , SSU , rbcL , and tufA ) and phylogenetically characterized. C. sorokiniana and C. vulgaris collected from Ulleung Island, South Korea, were homologous with other known species groups. Samples' fatty acid components were measured using GC/MS analysis in growth temperatures of 10 °C, 25 °C, and 35 °C. The growth rate of C. sorokiniana strains was higher than that of C. vulgaris under high-temperature conditions, confirming the potential industrial applicability of the former as feedstock material. Additionally, saturated fatty acid contents and productivities increased as biological resources of the C. sorokiniana strains were higher than those of C. vulgaris under high light intensity and temperature conditions. These results suggest that the fatty acid components of C. sorokiniana strains may potentially be used as biological resources (e.g., feedstock).

show abstract

“…( Castenholz et al, 2015b ). Some studies have been conducted with representatives of the genus Limnothrix in order to verify exopolysaccharides (EPS) ( Khattar et al, 2010 ) and lipid production ( Economou et al, 2015 ; Aboim et al, 2016 ; Hong et al, 2016 ; Oliveira et al, 2018 ), suggesting that this cyanobacteria could be used as a source for compounds of biotechnological interest. Besides that, Limnothrix (strain AC0243) is able to produce a toxin which causes severe toxicity to developing vertebrates with injuries particularly noted in the brain, notochord and pancreas, showing strong similarities to amino acid beta- N -methylamino- L -alanine (BMAA) intoxication ( Daniels et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Insights Into Limnothrix sp. Metabolism Based on Comparative Genomics

et al. 2018

View full text Add to dashboard Cite

Currently only four genome sequences for Limnothrix spp. are publicly available, and information on the genetic properties of cyanobacteria belonging to this genus is limited. In this study, we report the draft genome of Limnothrix sp. CACIAM 69d, isolated from the reservoir of a hydroelectric dam located in the Amazon ecosystem, from where cyanobacterial genomic data are still scarce. Comparative genomic analysis of Limnothrix revealed the presence of key enzymes in the cyanobacterial central carbon metabolism and how it is well equipped for environmental sulfur and nitrogen acquisition. Additionally, this work covered the analysis of Limnothrix CRISPR-Cas systems, pathways related to biosynthesis of secondary metabolites and assembly of extracellular polymeric substances and their exportation. A trans-AT PKS gene cluster was identified in two strains, possibly related to the novel toxin Limnothrixin biosynthesis. Overall, the draft genome of Limnothrix sp. CACIAM 69d adds new data to the small Limnothrix genome library and contributes to a growing representativeness of cyanobacterial genomes from the Amazon region. The comparative genomic analysis of Limnothrix made it possible to highlight unique genes for each strain and understand the overall features of their metabolism.

show abstract

Characterization of a Korean Domestic Cyanobacterium Limnothrix sp. KNUA012 for Biofuel Feedstock

Cited by 5 publications

References 30 publications

GC-MS profiling, anti-oxidant and anti-diabetic assessments of extracts from microalgae Scenedesmus falcatus (KU.B1) and Chlorella sorokiniana (KU.B2)

GC-MS profiling, anti-oxidant and anti-diabetic assessments of extracts from microalgae Scenedesmus falcatus (KU.B1) and Chlorella sorokiniana (KU.B2)

Characterization of Chlorella sorokiniana and Chlorella vulgaris fatty acid components under a wide range of light intensity and growth temperature for their use as biological resources

Insights Into Limnothrix sp. Metabolism Based on Comparative Genomics

Contact Info

Product

Resources

About