Characterization of a key aminoglycoside phosphotransferase in gentamicin biosynthesis

Specificity and Promiscuity at the Branch Point in Gentamicin Biosynthesis

“…Recent in vitro evidence using a substrate analog (Shao et al., 2013) implicates the phosphotransferase GenP in initiating this process, through phosphorylation at the C-3′ hydroxy group. Sisomicin, an antibiotic naturally produced by M. inyoensis (Reimann et al., 1974), is a 4′,5′-dehydro-derivative of gentamicin C1a.…”

The Bifunctional Enzyme, GenB4, Catalyzes the Last Step of Gentamicin 3’,4’-Di-Deoxygenation via Reduction and Transamination Activities

“…Our present in vitro catalytic findings demonstrate that (2) was verdamicin C2a and (3) was verdamicin C2. Based on the retention times of HPLC-ELSD, we hypothesized that (6) was VF3-1a(S) and complex such that they were the same as those in the wild-type strain, and △genK△ genB4::genB4 restored the productions of gentamicin C1a (9) and C2b such that they were the same as those in the original strain of △genK.…”

“…Lei et al demonstrated that the phosphotransferase, GenP, catalyzes the first step of 3',4'-di-deoxygenation in gentamicin biosynthesis [9]. Guo et al disrupted genB3 and genB4 separately in wild-type M. echinospora and the products accumulated into JI-20A and JI-20B; additionally, they demonstrated that GenB1, GenB2, GenB3, and GenB4 are pyridoxal-5'-phosphate (PLP)-dependent aminotransferases [6].…”

Virtual Force Term Based on the Velocity Distribution Surrounding a Spherical Particle Settling in a Simple Shear Flow

“…GenP catalyzes 3′‐OH phosphorylation of JI‐20A. This is possibly the first step of 3′,4′‐bisdehydroxylation in gentamicin biosynthesis 13. When we introduced an in‐frame deletion in gen P of the M. echinospora wild‐type (WT) strain, the phenotypic characteristics of the mutant Δ gen P strain, including colony color and shape, mycelium morphology, and growth rates, were not changed.…”

“…GenQ catalyzes the oxidation of X2 and G418 11. 12 GenP catalyzes the 3′‐OH phosphorylation step of JI‐20A, which is possibly the first step for C‐3′ dehydroxylation 13…”