Characterization of a<i>Saccharomyces cerevisiae</i>Mutant with Pseudohyphae and Cloning of a Gene Complementing the Mutation

Maneesri, Jaruwan; Azuma, Masayuki; Torii, S.; Igarashi, Koichi; Ooshima, Hiroshi

doi:10.1271/bbb.67.517

Cited by 6 publications

(4 citation statements)

References 26 publications

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“…As in other mutants with a constitutive pseudohyphal growth (Blacketer et al 1994(Blacketer et al , 1995Gimeno and Fink 1994;Murray et al 1998;Maneesri et al 2003), Ps93 and Ps168 mutants carry monogenic recessive mutations responsible for their morphologic phenotype. The mutations reside in two different unlinked genes.…”

Section: Discussionmentioning

confidence: 83%

“…Consititutive pseudohyphal growth has been reported in S. cerevisiae mutants growing on solid (Blacketer et al 1993) and liquid (Maneesri et al 2003) rich media, and in different strains carrying mutations in ELM, CDC12, GRR1 (Blacketer et al 1994(Blacketer et al , 1995, PHD1 (Gimeno and Fink 1994), SUP70 (Murray et al 1998;Beeser and Cooper 1999), and SCL1 (Maneesri et al 2003) genes. Recently, Halme et al (2004) found that mutations at IRA1 and IRA2 loci, in a flo11 background, determine a constitutive pseudohyphal phenotype associated with wrinkled colony morphology.…”

Section: Introductionmentioning

confidence: 99%

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Induction and characterization of morphologic mutants in a natural Saccharomyces cerevisiae strain

et al. 2007

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Saccharomyces cerevisiae is a good model with which to study the effects of morphologic differentiation on the ecological behaviour of fungi. In this work, 33 morphologic mutants of a natural strain of S. cerevisiae, obtained with UV mutagenesis, were selected for their streak shape and cell shape on rich medium. Two of them, showing both high sporulation proficiency and constitutive pseudohyphal growth, were analysed from a genetic and physiologic point of view. Each mutant carries a recessive monogenic mutation, and the two mutations reside in unlinked genes. Flocculation ability and responsiveness to different stimuli distinguished the two mutants. Growth at 37 degrees C affected the cell but not the colony morphology, suggesting that these two phenotypes are regulated differently. The effect of ethidium bromide, which affects mitochondrial DNA replication, suggested a possible "retrograde action" of mitochondria in pseudohyphal growth.

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Section: Discussionmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Induction and characterization of morphologic mutants in a natural Saccharomyces cerevisiae strain

et al. 2007

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“…Screening for morphological mutants of a haploid strain of S. cerevisiae growing on a solid medium with isoamyl alcohol, Maneesri et al (2003) have found a mutant which presented large percentage of cell extensions than parental strain, in which the addition of a DNA fragment that included the wild-type SCL1 gene complemented its mutant morphological phenotype. Sequencing of the mutant SCL1 showed that glycine at position 226 in the Scl1 protein (a subunit of proteasomes) had been replaced by aspartic acid.…”

Section: Nutritional Status and Stresses Are Effectors Of Filamentousmentioning

confidence: 99%

Biotechnological implications of filamentation in Saccharomyces cerevisiae

Ceccato-Antonini

2008

Biotechnol Lett

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The genetics governing the morphological switch from round or ovoid cells to filamentous growth in Saccharomyces cerevisiae has received significant interest in relation to sensing and signaling pathways as well as the control of cell processes including budding, elongation and adhesion. Little is known about the environmental signals which trigger these morphological changes from a biotechnological point of view. This review aims to highlight the main causes of filamentous growth in S. cerevisiae in its industrial setting with the purpose of stimulating additional studies within this field.

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“…The SLAD medium (Maneesri et al 2003) was used to characterize the influence of nitrogen deprivation upon pseudohyphal formation (0.67% yeast nitrogen base without amino acids and ammonium sulphate, 50 lM ammonium sulphate, 2% glucose and 2% agar). The glucose and agar were autoclaved; all other ingredients were sterilized by filtration.…”

Section: Nitrogen Deprivation-induced Filamentationmentioning

confidence: 99%

Characterization of industrial strains of Saccharomyces cerevisiae exhibiting filamentous growth induced by alcohols and nutrient deprivation

Silva

Horii

Miranda

et al. 2006

World J Microbiol Biotechnol

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The use of microorganisms in biotechnology is an important economic area of interest in Brazil, especially the use of Saccharomyces cerevisiae in the baking and alcohol fermentation industries. Dimorphism in S. cerevisiae (cell morphology alterations from budding cells to filamentous structures) has been observed in conditions of nitrogen and carbon deprivation and in the presence of fusel alcohols. This can be described as a defense mechanism that allows the yeast to forage for nutrients through cell elongation, hyphal formation and invasive growth. In this work fifteen industrial strains of S. cerevisiae (including haploid and diploid strains) isolated from the fermentative process for alcohol production were characterized for filamentation on solid culture media under growth conditions of carbon-and nitrogen-deprivation and in the presence of fusel alcohols. The majority of strains showed filamentation induced by isoamyl alcohol, butanol, isopropanol and isobutanol, but not by methanol. In rich medium (YEPD), both haploid and diploid strains showed invasive growth, although this kind of filamentous growth was more common in haploid strains. Similar results were observed when fructose or mannose was used as the sole carbon source. In nitrogen-deficient medium (SLAD) the strains did not filament. The results obtained indicate that the filamentation induced by higher alcohols and carbon deprivation (specially carbon) is a common process in industrial strains of S. cerevisiae contributing towards their maintenance/survival in adverse conditions.

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Characterization of aSaccharomyces cerevisiaeMutant with Pseudohyphae and Cloning of a Gene Complementing the Mutation

Cited by 6 publications

References 26 publications

Induction and characterization of morphologic mutants in a natural Saccharomyces cerevisiae strain

Induction and characterization of morphologic mutants in a natural Saccharomyces cerevisiae strain

Biotechnological implications of filamentation in Saccharomyces cerevisiae

Characterization of industrial strains of Saccharomyces cerevisiae exhibiting filamentous growth induced by alcohols and nutrient deprivation

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