Characterization of a Highly Repetitive Sequence Conserved Among the North American Morone Species

Abstract

: A highly repetitive DNA sequence family from the genome of the North American Morone has been cloned and characterized. This family, first identified as a HindIII repetitive element, is composed of repeat units that range from 285 to 288 bp in length and comprise approximately 5.5% of the genome. The copy number of the repeat was estimated to be 1.85 x 10(5) per haploid genome set. Data from Southern blot analyses demonstrated that the HindIII repetitive element was tandemly organized. Sequence analysis of six cloned repeat monomers from each of the four North American Morone species, M. saxatilis, M. chrysops, M. americana, and M. mississippiensis, revealed a high degree of conservation of the monomeric unit. The intraspecific sequence variation ranged from 3.2% to 5.4%. A similar level of variation was detected between cloned monomers from the same individual, suggesting that most of the intraspecies variation may be due to variation among copies of the repeat. The interspecific sequence variation ranged from less than 4.6% between M. americana and M. mississippiensis to approximately 16% between the other Morone species pairs. Phylogenetic analysis of the repetitive element nucleotide sequences indicated that M. americana and M. mississippiensis were more closely related to each other than to any other pairs of Morone species. In addition, we reconstructed the Morone phylogeny using 22 previously described morphologic characters. Congruent relationships were obtained between both sets of data. The data suggest that the genus Morone is composed of two sets of sister taxa, M. saxatilis:M. chrysops and M. americana:M. mississippiensis.

The complete mitochondrial genome sequence of white perch Morone americana (Perciformes, Moronidae)

“…The tree based on the concatenated nucleotide sequences of three mitochondrial genes showed similar results, with M. americana as sister taxon to M. mississippiensis , consistent with the conclusion of Leclerc et al. ( 1999 ) based on repetitive nuclear DNA sequences. The mitogenome of M. americana represents a useful resource for marker development for population studies, as well as for studies of phylogeny of Moronidae and related species.…”

Application of Microsatellite Markers in Conservation Genetics and Fisheries Management: Recent Advances in Population Structure Analysis and Conservation Strategies

“…This is evidenced by the analysis of flanking regions of the single copy nuclear DNA locus (Tmo M27) and by studying the parental lineages in six other microsatellite loci. Leclerc et al [69] had cloned and characterized a highly repetitive DNA sequence from the genome of the North American Morone saxatilis that was used to distinguish the four other species. Neff et al [70] described 10 microsatellite loci from blue gill ( Lepomis macrochirus ) and discussed their evolution within the family Centrarchidae.…”

A Glimpse into the Satellite DNA Library in Characidae Fish (Teleostei, Characiformes)

“…Up to now, most studies of satDNAs in fish genomes have focused on the development of chromosomal markers for evolutionary studies on B and sex chromosomes ( Mestriner et al, 2000 ; Jesus et al, 2003 ; Vicari et al, 2010 ; Utsunomia et al, 2016 ). However, the evolutionary trends of satDNAs in closely related fish species have not yet been well evaluated, mainly if we consider that almost all discovered satDNA analyzed until now seemed to represent species- or genus-specific sequences ( Garrido-Ramos et al, 1999 ; Leclerc et al, 1999 ; de la Herrán et al, 2001 ; Lanfredi et al, 2001 ; Robles et al, 2004 ; Martins et al, 2006 ).…”