Characterization of a highly pathogenicBacillus thuringiensis strain isolated from common cockchafer,Melolontha melolontha

Katı, Hatice; Sezen, Kazım; Demirbağ, Zihni

doi:10.1007/bf02932153

Cited by 17 publications

(16 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The antibacterial substance producer strain used in this study is Sm-Mm3 isolated from Melolontha melolontha (Coleoptera: Scarabaeidae), a serious hazelnut pest in the Black Sea region of Turkey (Kati et al, 2007;Sezen et al, 2007). The producer strain and indicator strain (Bacillus thuringiensis subsp.…”

Section: Microbial Strains and Growth Conditionsmentioning

confidence: 99%

Purification and characterization of an antibacterial substance produced by pest-originated Serratia marcescens Mm3

Uğraş¹,

Sezen²,

Katı³

et al. 2014

Turk J Biol

View full text Add to dashboard Cite

IntroductionSince the exploration of penicillin as a natural antibiotic and its subsequent production on a massive scale, antibiotics have revolutionized approaches to human health (Bennett and Chung, 2001). They have been used in human healthcare and in different fields such as the agricultural industry and veterinary medicine. As a result of the widespread use of antibacterials, antibiotic-resistant pathogens have been widely and continuously reported by the World Health Organization (WHO, 2000). In addition, synthetic chemicals that can negatively affect the environment and human and animal health have been used as antimicrobials (Hodgson and Levi, 1996;Zwir-Ferenc and Biziuk, 2004). Therefore, researchers always aim to find safe alternatives, such as natural and environmentally friendly antimicrobials (Tronsmo and Hjeljord, 1998).The need for novel antimicrobials to combat increasing antibiotic resistance in pathogenic bacteria has stimulated the exploration of nontraditional sources, such as terrestrial actinomycetes or fungi (Berdy, 2005). Marine organisms have been a rich source of novel antibacterial compounds (Zhang et al., 2005). Unfortunately, knowledge about antimicrobial substances of microorganisms isolated from pests is very limited.Serratia is a genus of gram-negative, facultative anaerobic, and rod-shaped bacteria of the family Enterobacteriaceae. The most common species in the genus, S. marcescens, is normally the only pathogen, and usually causes nosocomial infections (Hejazi and Falkiner, 1997). The ability of S. marcescens to inhabit a wide variety of ecological niches has been linked to its extracellular products, including proteases, chitinases, nucleases, lipases, wetting agents, bacteriocins, and the bright red pigment 2-methyl-3-penthyl-6-methoxyprodigiosin or prodigiosin (Matsuyama et al., 1995;Thomson et al., 1997). In addition, the genus Serratia is able to produce β-lactam antibiotic and 1-carbapen-2-em-3-carboxylic acid (carbapenem; Car) (Williams and Quadri, 1980;Parker et al., 1982). Furthermore, secondary metabolites such as antibiotics and bacteriocins produced by the genus Serratia are very important in various industrial fields because of their antibacterial activities. Additionally, several Serratia strains have the potential to control insect pests (Sezen and Demirbag, 2006;Jeong et al., 2010) and plant-pathogenic bacteria (Ordentlich et al., 1998).In the current study, in order to determine a novel antibacterial substance as an alternative to existing antimicrobials, we characterized an antibacterial substance

show abstract

Section: Microbial Strains and Growth Conditionsmentioning

confidence: 99%

Purification and characterization of an antibacterial substance produced by pest-originated Serratia marcescens Mm3

Uğraş¹,

Sezen²,

Katı³

et al. 2014

Turk J Biol

View full text Add to dashboard Cite

show abstract

“…They were isolated from six different insects belonging to Coleoptera, significant pests of hazelnut in the Black Sea Region of Turkey. Test bacteria used in this study were isolated, identified and assayed for insecticidal activity in previous studies (Sezen & Demirbag 1999;Demir et al 2002;Sezen et al 2004Sezen et al , 2007Sezen et al , 2008aBahar & Demirbag 2007;Kati et al, 2007). The bacterial strains used as indicator bacteria are shown in Table 2.…”

Section: Bacterial Strains and Growth Conditionsmentioning

confidence: 99%

Screening antibacterial activity of entomopathogenic bacteria isolated from pests of hazelnut

Uğraş

Demirbağ

2013

Biologia

View full text Add to dashboard Cite

Thirty-seven entomopathogenic bacteria isolated from six common pests of hazelnut in the Black Sea Region of Turkey have been screened for their potential of antibacterial substance production against indicator bacteria by the agar spot assay and well diffusion assay. Results indicated that 13.5% of entomopathogenic bacteria, Pseudomonas fluorescens (Pf-Xd1), Bacillus polymyxa (Bp-Ar2), Bacillus thuringiensis (Bt-Bn1), Serratia marcescens (Sm-Mm3) and Pseudomonas flourescens (Pf-Aa4) isolated from pests of Xyleborus dispar, Anoplus roboris, Balaninus nucum, Melolontha melolontha and Agelastica alni, respectively, showed significant levels of inhibitor activities against indicator bacteria. Well diffusion assay showed that supernatants of Bp-Ar2, Bt-Bn1 and Sm-Mm3 have antibacterial activity, whereas Pf-Xd1 and Pf-Aa4 did not show any activity. Furthermore, the antibacterial activity of the substance produced by the Bp-Ar2 has a narrow spectrum, whereas those of Bt-Bn1 and Sm-Mm3 exhibit broad spectrum. The production of these antibacterial substances were similarly determined at early logarithmic phase in the growth cycle of three bacteria and continued until the beginning of the stationary phase as primer metabolite. In addition, optimal pH (at 7-9 forBt-Bn1 and 5-9 forSm-Mm3), medium (Muller Hinton broth forBt-Bn1 and Luria Bertani broth forSm-Mm3), temperature (25 • C forBt-Bn1 and Sm-Mm3) and production time (24h forBt-Bn1 and 72h forSm-Mm3) of these substances were determined. Our results demonstrate that entomopathogenic bacteria are a potential source of antibacterial substances.

show abstract

“…It is ubiquitous in soil, phyllosphere, stored food, dead insects, air, water etc. but soil is the primary habitat 1,2 . Out of 11000 soils of 30 countries, Bt (27000) habited in 50-70% total, 94.3% Asian and 87.1% agricultural soils [1][2][3][4] .…”

Section: Introductionmentioning

confidence: 99%

“…but soil is the primary habitat 1,2 . Out of 11000 soils of 30 countries, Bt (27000) habited in 50-70% total, 94.3% Asian and 87.1% agricultural soils [1][2][3][4] . The Bt infects all insect orders and in the rice fi elds more than 50% insects recorded infection [1][2][3] .…”

Section: Introductionmentioning

confidence: 99%

“…Out of 11000 soils of 30 countries, Bt (27000) habited in 50-70% total, 94.3% Asian and 87.1% agricultural soils [1][2][3][4] . The Bt infects all insect orders and in the rice fi elds more than 50% insects recorded infection [1][2][3] . However, information on the diversity and distribution of the Bt in South-East Asian countries, especially in the rice fi eld soils of India, are negligible [3][4][5] .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Diversity of Bacillus thuringiensis in the rice field soils of different ecologies in India

Das

Dangar

2007

Indian J Microbiol

View full text Add to dashboard Cite

Diversity of the Bacillus thuringiensis (Bt) in the rice fi eld soils of different ecologies viz. the island (Port Blair), the Himalayan (Srinagar), brackish water (Mahe) and coastal mesophilic (Mangalore) habitats was analyzed by phenotypic characterization of 5, 66, 14 and 54 Bt isolates, respectively. The Bt isolates produced either monotypic (bipyramidal or spherical) or heterotypic (polymorphic-bipyramidal or bipyramidal-rhomboidal) crystals. The organisms were generally resistant to the penicillin group of antibiotics, tolerated 5-12% NaCl and 0.5M Na-acetate. The Bt isolates contained 1-5 plasmids of 0.89-58.61 kbp sizes. The plasmid profi les had no correlation with crystal morphology or salt tolerance of different bacteria. Each soil was inhabited by different types of Bt. Two Bt strains of Mangalore and one strain each of the other places were phenotypically similar. One Bt strain each of Port Blair and Srinagar was different from all other strains.

show abstract

Characterization of a highly pathogenicBacillus thuringiensis strain isolated from common cockchafer,Melolontha melolontha

Cited by 17 publications

References 18 publications

Purification and characterization of an antibacterial substance produced by pest-originated Serratia marcescens Mm3

Purification and characterization of an antibacterial substance produced by pest-originated Serratia marcescens Mm3

Screening antibacterial activity of entomopathogenic bacteria isolated from pests of hazelnut

Diversity of Bacillus thuringiensis in the rice field soils of different ecologies in India

Contact Info

Product

Resources

About