Characterization of a gibberellin sensitive dwarf mutant of barley (Hordeum vulgare L.,Mut. Dornburg 576)

Hentrich, W.; Bergner, C.; Sembdner, G.

doi:10.1007/bf01806049

Cited by 10 publications

(9 citation statements)

References 8 publications

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“…4) are typical of 3␤-hydroxylase mutants that have been isolated in several species. There are two GA-responsive dwarf mutants in barley that have been studied in some detail (Hentrich et al, 1985;Boother et al, 1991), and both of these are allelic with the grd1 locus described here (P.M. Chandler, unpublished data).…”

Section: Discussionmentioning

confidence: 86%

Gibberellin Dose-Response Curves and the Characterization of Dwarf Mutants of Barley

1999

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Dose-response curves relating gibberellin (GA) concentration to the maximal leaf-elongation rate (LER max ) defined three classes of recessive dwarf mutants in the barley (Hordeum vulgare L.) 'Himalaya.' The first class responded to low (10 ؊8 -10 ؊6 M) [GA 3 ] (as did the wild type). These grd (GA-responsive dwarf) mutants are likely to be GA-biosynthesis mutants. The second class of mutant, gse (GA sensitivity), differed principally in GA sensitivity, requiring approximately 100-fold higher [GA 3 ] for both leaf elongation and ␣-amylase production by aleurone. This novel class may have impaired recognition between the components that are involved in GA signaling. The third class of mutant showed no effect of GA 3 on the LER max . When further dwarfed by treatment with a GA-biosynthesis inhibitor, mutants in this class did respond to GA 3 , although the LER max never exceeded that of the untreated dwarf. These mutants, called elo (elongation), appeared to be defective in the specific processes that are required for elongation rather than in GA signaling. When sln1 (slender1) was introduced into these different genetic backgrounds, sln was epistatic to grd and gse but hypostatic to elo. Because the rapid leaf elongation typical of sln was observed in the grd and gse backgrounds, we inferred that rapid leaf elongation is the default state and suggest that GA action is mediated through the activity of the product of the Sln gene.

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Section: Discussionmentioning

confidence: 86%

Gibberellin Dose-Response Curves and the Characterization of Dwarf Mutants of Barley

1999

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“…These two hormones are also involved in the control of stem height, fertility, and maturation of the seeds (Barnett, 1972;Michael and Seiler-Kelbitsh, 1972;Kaufman and Jones, 1974;Koning et al, 1977;Jung, 1984;Hentrich et al, 1985;Pharis and King, 1985;Morgan and Quinby, 1987). Therefore, it seems likely that the changes in relative stem height observed in adapted plants is related to a modification of the hormonal balance following expression of the adaptation response.…”

Section: Adaptation Resistance and Expression Of The Reproductive Cmentioning

confidence: 96%

Induced Modifications in Reproductive Traits of Salt-Treated Plants of Sorghum Bicolor

Amzallag

1998

Israel J Plant Sci

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Plants of Sorghum bicolor were grown hydroponically in a solution containing 150 mM NaCl, and the reproductive characters were analyzed at the end of the life cycle. A subpopulation was exposed to salinity on day 8 following germination (early-exposed plants), and another subpopulation was first exposed to salinity on day 21 following germination (late-exposed plants). Fertility and stem height, as determined per g shoot dry weight (DW), were similar for control and late-exposed plants. However, these parameters were largely modified in early-exposed plants.The population of late-exposed plants displayed an increase in phenotypic variability. However, the monomodal structure of the population remained similar to that of the population of control plants. In contrast, a large increase in diversity was observed in the population of early-exposed plants, and the frequency distribution of the reproductive characters appeared as plurimodal for this population.Early exposure to NaCl has been previously shown to induce an increase in salinity tolerance of the plant (termed salt adaptation). This effect was not observed for late-exposed plants. The significance of the induced modifications in reproductive characters is discussed in the light of the salt adaptation response in Sorghum.

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“…The dwarf mutant 'Dornburg 576' (dbg 576) originated from the barley variety Saale [13]. Mutant and wild-type (cv.…”

Section: Plant Materialsmentioning

confidence: 99%

“…Dose response curves for mutant and wild-type leaf sheaths and seedling growth have shown a higher sensitivity to GA 3 of the mutant compared to the wild type [ 13,29]. Wild-type plants contain higher levels of GAs and could have a reduced sensitivity to the hormone.…”

Section: Es2amentioning

confidence: 99%

“…In several species, GAs are also involved in the control of leaf growth. GA 3 alters leaf shape and growth, particularly when applied to GAdeficient mutants of several plants, including barley, tomato and Arabidopsis [13,21]. These mutants have low endogenous levels of GAs, associated with reduced internode and leaf length, abnormal sex expression and altered seed dormancy.…”

Section: Introductionmentioning

confidence: 99%

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GA3-regulated cDNAs from Hordeum vulgare leaves

Speulman¹,

Salamini²

1995

Plant Mol Biol

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The barley mutant dbg 576 shows an extreme vegetative dwarf phenotype. This is reversed after the application of GA3 which also induces the mutant florets to become partly fertile. cDNA clones ES1A and ES2A were isolated by differential screening with subtracted probes from a DNA library prepared from mutant leaf blades after GA3 treatment. Both the ES1A and the ES2A mRNA level increases as early as 30 min after GA3 treatment and decreases later. Accumulation of ES1A and ES2A mRNAs is leaf blade-specific and both are ca. 750 nucleotides long. ES1A encodes a protein of approximately 6 kD which shows a significant homology with mammalian epidermal growth factors (EGFs). ES2A encodes a protein of 22 kDa with homology, in regions with potential amphiphilic helices, with the D7 family of late embryogenesis-abundant proteins (LEA).

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Characterization of a gibberellin sensitive dwarf mutant of barley (Hordeum vulgare L.,Mut. Dornburg 576)

Cited by 10 publications

References 8 publications

Gibberellin Dose-Response Curves and the Characterization of Dwarf Mutants of Barley

Gibberellin Dose-Response Curves and the Characterization of Dwarf Mutants of Barley

Induced Modifications in Reproductive Traits of Salt-Treated Plants of Sorghum Bicolor

GA3-regulated cDNAs from Hordeum vulgare leaves

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