Characterization of a Gamma Radiation-Induced Salt-Tolerant Silage Maize Mutant

Cho, Chuloh; Kim, Kyung Hwa; Choi, Mansoo; Chun, Jae-Buhm; Seo, Mi-Suk; Jeong, Namhee; Jin, Mina; Son, Beom-Young; Kim, and Dool-Yi

doi:10.9787/kjbs.2019.51.4.318

Cited by 4 publications

(4 citation statements)

References 26 publications

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“…Because CIPK31 inhibits both RBOHA and CatC and promotes ROS levels, further genetic study with a combination of rboha × catc will be interesting to dissect the mechanism of CIPK31‐mediated ROS homeostasis. Taken together, CIPK31 confers resistance to ShB (Figures 1a–f and 2a–e ), rice blast (Lin et al, 2021 ), and bacterial blight (Figure S2e–h ), and plays roles in growth and development in plants (Cho et al, 2019 ; Nagarjuna et al, 2016 ; Peng et al, 2018 ; Piao et al, 2010 ). However, clearly, CIPK31 interacts with CatC to modulate ROS homeostasis to promote rice resistance.…”

Section: Discussionmentioning

confidence: 94%

Calcineurin B‐like interacting protein kinase 31 confers resistance to sheath blight via modulation of ROS homeostasis in rice

Chen

Lin

et al. 2023

Molecular Plant Pathology

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Sheath blight (ShB) severely threatens rice cultivation and production; however, the molecular mechanism of rice defence against ShB remains unclear. Screening of transposon Ds insertion mutants identified that Calcineurin B‐like protein‐interacting protein kinase 31 ( CIPK31 ) mutants were more susceptible to ShB, while CIPK31 overexpressors ( OX ) were less susceptible. Sequence analysis indicated two haplotypes of CIPK31 : Hap_1, with significantly higher CIPK31 expression, was less sensitive to ShB than the Hap_2 lines. Further analyses showed that the NAF domain of CIPK31 interacted with the EF‐hand motif of respiratory burst oxidase homologue (RBOHA) to inhibit RBOHA‐induced H 2 O 2 production, and RBOHA RNAi plants were more susceptible to ShB. These data suggested that the CIPK31‐mediated increase in resistance is not associated with RBOHA. Interestingly, the study also found that CIPK31 interacted with catalase C (CatC); cipk31 mutants accumulated less H 2 O 2 while CIPK31 OX accumulated more H 2 O 2 compared to the wild‐type control. Further analysis showed the interaction of the catalase domain of CatC with the NAF domain of CIPK31 by which CIPK31 inhibits CatC activity to accumulate more H 2 O 2 .

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Section: Discussionmentioning

confidence: 94%

Calcineurin B‐like interacting protein kinase 31 confers resistance to sheath blight via modulation of ROS homeostasis in rice

Chen

Lin

et al. 2023

Molecular Plant Pathology

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“…In monocotyledons plants, 740 and 1384 viable mutants were isolated in rice M 2 population (90,386 plants) that derived from gamma rays (100–500 Gy) and electron beam (200–600 Gy) irradiated seeds, the mutation frequency was 2.35% [ 51 ]. In the case of maize, 10 salt-tolerant lines were isolated from 2248 M 2 plants that derived from seeds irradiated by gamma rays at 100 Gy, the mutation frequency was 0.44% (4.44 × 10 −5 per dose) [ 52 ]. In barley, one dwarf mutant was isolated from 10,000 M 2 individuals that derived from seeds irradiated by 200 Gy gamma rays, the mutation frequency was 0.01% (0.05 × 10 −5 per dose) [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Frequency and Spectrum of Mutations Induced by Gamma Rays Revealed by Phenotype Screening and Whole-Genome Re-Sequencing in Arabidopsis thaliana

Feng

Wang

et al. 2022

IJMS

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Genetic variations are an important source of germplasm diversity, as it provides an allele resource that contributes to the development of new traits for plant breeding. Gamma rays have been widely used as a physical agent for mutation creation in plants, and their mutagenic effect has attracted extensive attention. However, few studies are available on the comprehensive mutation profile at both the large-scale phenotype mutation screening and whole-genome mutation scanning. In this study, biological effects on M1 generation, large-scale phenotype screening in M2 generation, as well as whole-genome re-sequencing of seven M3 phenotype-visible lines were carried out to comprehensively evaluate the mutagenic effects of gamma rays on Arabidopsis thaliana. A total of 417 plants with visible mutated phenotypes were isolated from 20,502 M2 plants, and the phenotypic mutation frequency of gamma rays was 2.03% in Arabidopsis thaliana. On average, there were 21.57 single-base substitutions (SBSs) and 11.57 small insertions and deletions (InDels) in each line. Single-base InDels accounts for 66.7% of the small InDels. The genomic mutation frequency was 2.78 × 10−10/bp/Gy. The ratio of transition/transversion was 1.60, and 64.28% of the C > T events exhibited the pyrimidine dinucleotide sequence; 69.14% of the small InDels were located in the sequence with 1 to 4 bp terminal microhomology that was used for DNA end rejoining, while SBSs were less dependent on terminal microhomology. Nine genes, on average, were predicted to suffer from functional alteration in each re-sequenced line. This indicated that a suitable mutation gene density was an advantage of gamma rays when trying to improve elite materials for one certain or a few traits. These results will aid the full understanding of the mutagenic effects and mechanisms of gamma rays and provide a basis for suitable mutagen selection and parameter design, which can further facilitate the development of more controlled mutagenesis methods for plant mutation breeding.

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“…Several mutants displayed no pigments on their leaves and stems or trichomes on their leaves; these were isolated from Arabidopsis thaliana, which was irradiated using a carbon-ion beam [33]. Only ten salt-tolerant maize lines derived from gamma-ray irradiation were isolated from over 2000 plants [34]. Although the criteria for screening phenotypes varied slightly among different studies, these results still prove that different mutation phenotypes occur with different frequencies.…”

Section: Phenotypic Spectra Of Carbon-ion-beam Irradiation and Gamma-...mentioning

confidence: 99%

Comparison and Characterization of Phenotypic and Genomic Mutations Induced by a Carbon-Ion Beam and Gamma-ray Irradiation in Soybean (Glycine max (L.) Merr.)

Feng

Chen

et al. 2023

IJMS

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Soybean (Glycine max (L.) Merr.) is a nutritious crop that can provide both oil and protein. A variety of mutagenesis methods have been proposed to obtain better soybean germplasm resources. Among the different types of physical mutagens, carbon-ion beams are considered to be highly efficient with high linear energy transfer (LET), and gamma rays have also been widely used for mutation breeding. However, systematic knowledge of the mutagenic effects of these two mutagens during development and on phenotypic and genomic mutations has not yet been elucidated in soybean. To this end, dry seeds of Williams 82 soybean were irradiated with a carbon-ion beam and gamma rays. The biological effects of the M1 generation included changes in survival rate, yield and fertility. Compared with gamma rays, the relative biological effectiveness (RBE) of the carbon-ion beams was between 2.5 and 3.0. Furthermore, the optimal dose for soybean was determined to be 101 Gy to 115 Gy when using the carbon-ion beam, and it was 263 Gy to 343 Gy when using gamma rays. A total of 325 screened mutant families were detected from out of 2000 M2 families using the carbon-ion beam, and 336 screened mutant families were found using gamma rays. Regarding the screened phenotypic M2 mutations, the proportion of low-frequency phenotypic mutations was 23.4% when using a carbon ion beam, and the proportion was 9.8% when using gamma rays. Low-frequency phenotypic mutations were easily obtained with the carbon-ion beam. After screening the mutations from the M2 generation, their stability was verified, and the genome mutation spectrum of M3 was systemically profiled. A variety of mutations, including single-base substitutions (SBSs), insertion–deletion mutations (INDELs), multinucleotide variants (MNVs) and structural variants (SVs) were detected with both carbon-ion beam irradiation and gamma-ray irradiation. Overall, 1988 homozygous mutations and 9695 homozygous + heterozygous genotype mutations were detected when using the carbon-ion beam. Additionally, 5279 homozygous mutations and 14,243 homozygous + heterozygous genotype mutations were detected when using gamma rays. The carbon-ion beam, which resulted in low levels of background mutations, has the potential to alleviate the problems caused by linkage drag in soybean mutation breeding. Regarding the genomic mutations, when using the carbon-ion beam, the proportion of homozygous-genotype SVs was 0.45%, and that of homozygous + heterozygous-genotype SVs was 6.27%; meanwhile, the proportions were 0.04% and 4.04% when using gamma rays. A higher proportion of SVs were detected when using the carbon ion beam. The gene effects of missense mutations were greater under carbon-ion beam irradiation, and the gene effects of nonsense mutations were greater under gamma-ray irradiation, which meant that the changes in the amino acid sequences were different between the carbon-ion beam and gamma rays. Taken together, our results demonstrate that both carbon-ion beam and gamma rays are effective techniques for rapid mutation breeding in soybean. If one would like to obtain mutations with a low-frequency phenotype, low levels of background genomic mutations and mutations with a higher proportion of SVs, carbon-ion beams are the best choice.

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Characterization of a Gamma Radiation-Induced Salt-Tolerant Silage Maize Mutant

Cited by 4 publications

References 26 publications

Calcineurin B‐like interacting protein kinase 31 confers resistance to sheath blight via modulation of ROS homeostasis in rice

Calcineurin B‐like interacting protein kinase 31 confers resistance to sheath blight via modulation of ROS homeostasis in rice

Frequency and Spectrum of Mutations Induced by Gamma Rays Revealed by Phenotype Screening and Whole-Genome Re-Sequencing in Arabidopsis thaliana

Comparison and Characterization of Phenotypic and Genomic Mutations Induced by a Carbon-Ion Beam and Gamma-ray Irradiation in Soybean (Glycine max (L.) Merr.)

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