Characterization of a galectin-like activity from the parasitic nematode, Haemonchus contortus, which modulates ovine eosinophil migration in vitro

Turner, Darryl; Wildblood, L. A.; Inglis, Neil F.; Jones, Douglas

doi:10.1016/j.vetimm.2007.11.002

Cited by 48 publications

(35 citation statements)

References 28 publications

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“…In parasites, it has been hypothesized that lectins in the ES products may be able to subvert the host immune system by binding to carbohydrate moieties on the surface of host cells (41,42). In particular, host galectins have been shown to modulate a range of immune responses including cell recruitment, activation, development, and apoptosis (43,44), and the recent report of the eosinophil chemokinetic activity of galectins from H. contortus (45) suggests that parasite galectins may be mimicking host proteins. The relative abundance of lectins in A. caninum ES products suggests an important role for these proteins in parasite-host interactions.…”

Section: Figmentioning

confidence: 99%

Proteomics Analysis of the Excretory/Secretory Component of the Blood-feeding Stage of the Hookworm, Ancylostoma caninum

Mulvenna

Hamilton

Nagaraj

et al. 2009

Molecular & Cellular Proteomics

165

166

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Hookworms are blood-feeding intestinal parasites of mammalian hosts and are one of the major human ailments affecting ϳ600 million people worldwide. These parasites form an intimate association with the host and are able to avoid vigorous immune responses in many ways including skewing of the response phenotype to promote parasite survival and longevity. The primary interface between the parasite and the host is the excretory/secretory component, a complex mixture of proteins, carbohydrates, and lipids secreted from the surface or oral openings of the parasite. The composition of this complex mixture is for the most part unknown but is likely to contain proteins important for the parasitic lifestyle and hence suitable as drug or vaccine targets. Using a strategy combining the traditional technology of one-dimensional SDS-PAGE and the newer fractionation technology of OFFGEL electrophoresis we identified 105 proteins from the excretory/secretory products of the blood-feeding stage of the dog hookworm, Ancylostoma caninum. Highly represented among the identified proteins were lectins, including three C-type lectins and three ␤-galactoside-specific S-type galectins, as well as a number of proteases belonging to the three major classes found in nematodes, aspartic, cysteine, and metalloproteases. Interestingly 28% of the identified proteins were homologous to activation-associated secreted proteins, a family of cysteine-rich secreted proteins belonging to the sterol carrier protein/Tpx-1/Ag5/ PR-1/Sc-7 (TAPS) superfamily. Thirty-four of these proteins were identified suggesting an important role in host-parasite interactions. Other protein families identified included hyaluronidases, lysozyme-like proteins, and transthyretin-like proteins. This work identified a suite of proteins important for the parasitic lifestyle and provides new insight into the biology of hookworm infection.

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Section: Figmentioning

confidence: 99%

Proteomics Analysis of the Excretory/Secretory Component of the Blood-feeding Stage of the Hookworm, Ancylostoma caninum

Mulvenna

Hamilton

Nagaraj

et al. 2009

Molecular & Cellular Proteomics

165

166

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“…This hypothesis is reinforced by reports indicating that lectin-glycan binding is important for the infection and virulence processes of some parasites, e.g. Acanthamoeba castellanii (Garate et al, 2006), H. contortus (Turner et al, 2008), L. (V.) braziliensis (Rebello et al, 2009) and T. gondii (Fauquenoy et al, 2008) …”

Section: Remarks On the Isolation Of Proteins By Lectin Affinity Chromentioning

confidence: 81%

Affinity-Based Methods for the Separation of Parasite Proteins

Alves¹,

Silva²,

Oliveira³

et al. 2012

Affinity Chromatography

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“…There may be smaller contributions in other experiments, as it has been suggested that cellular migration is a mixture of chemotactic and chemokinetic movement (Keller and Sorkin 1966;Wilkinson and Haston 1988). In addition to chemotaxins, a chemokinetic factor was shown to be present in soluble extracts of L3 H. contortus and identified as a galectin-like molecule (Turner et al 2008).…”

Section: Discussionmentioning

confidence: 96%

“…Those which have been characterised are diverse, and many are similar to mammalian proteins: the ECF activity in Anopheles mosquito saliva is a member of the chitinase family with an MW around 66 kDa. (Owhashi et al 2008); galectin-like proteins are the eosinophil chemotaxins in abomasal nematodes (Turner et al 2008); an NCF in D. immitis extracts contains the amino acid sequence Met-Phe-Lys (Owhashi et al 1993); the NCF in T. foetus is a superoxide dismutase (Granger et al 1997), and the A. suum NCF appeared to act via IL-8 receptors rather than fMLP receptors (Falcone et al 2001). …”

Section: Discussionmentioning

confidence: 99%

“…Those which have been identified are very dissimilar both in structure and possible mode of action, e.g. ECF activity in Anopheles mosquito saliva (Owhashi et al 2008) is attributed to a member of the chitinase family, whereas the ECF is a galectin-like protein in abomasal nematodes (Turner et al 2008); an NCF in D. immitis extracts contains the amino acid sequence MetPhe-Lys (Owhashi et al 1993), whilst it is a superoxide dismutase in T. foetus (Granger et al 1997). …”

Section: Introductionmentioning

confidence: 99%

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Neutrophil and eosinophil chemotactic factors in the excretory/secretory products of sheep abomasal nematode parasites

2011

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Both eosinophil chemotactic factor (ECF) and neutrophil chemotactic factor (NCF) activities were demonstrated in excretory/secretory (ES) products and homogenates of Haemonchus contortus and Teladorsagia circumcincta larvae and adult worms in a modified checkerboard assay using a micro-chemotaxis chamber. Neutrophil chemotaxis was seen in 28 of 35 experiments and eosinophil chemotaxis in 20 of 38 experiments. Chemokinetic activity for neutrophils and eosinophils (accounting for 40-50% of total cell migration) was also apparent in only three parasite products for each cell type. Significant NCF activity was present in six of seven adult worm ES products (three of four from T. circumcincta and in all three from H. contortus) and ECF activity in four of five adult ES products, whereas fewer L3 incubates, particularly of T. circumcincta, contained chemotactic activity. All parasite homogenates, with one exception for ECF, were chemotactic for both neutrophils and eosinophils. The sequential use of cellulose ultrafiltration membranes of decreasing pore size did not identify precisely the molecular weight of the NCF and ECF but indicated that the active chemicals were greater than 10 kDa and probably greater than 30 kDa.

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Characterization of a galectin-like activity from the parasitic nematode, Haemonchus contortus, which modulates ovine eosinophil migration in vitro

Cited by 48 publications

References 28 publications

Proteomics Analysis of the Excretory/Secretory Component of the Blood-feeding Stage of the Hookworm, Ancylostoma caninum

Proteomics Analysis of the Excretory/Secretory Component of the Blood-feeding Stage of the Hookworm, Ancylostoma caninum

Affinity-Based Methods for the Separation of Parasite Proteins

Neutrophil and eosinophil chemotactic factors in the excretory/secretory products of sheep abomasal nematode parasites

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