Characterization of a galactose binding serum lectin from the Indian catfish, Clarias batrachus: Possible involvement of fish lectins in differential recognition of pathogens

Evaluation of Antimicrobial Potential of a Galactose-Specific Lectin in the Skin Mucus of African Catfish (Clarias gariepinus, Burchell, 1822) against some Aquatic Microorganisms

“…The intelectin catfish, Silurus asotus showed agglutination activity against the pathogenic bacterium Aeromonas salmonicida (Tsutsui et al, 2011). Dutta et al (2005) reported agglutination of Gram-negative bacteria, when Gram-positive and Gram-negative were incubated with catfish serum lectin. It was noted that incubation of the lectin at nanogram level brought about significant growth inhibition of various Aeromonas strains.…”

“…Agglutination assays with the formalin-killed cells was performed in the microtitre plates. An equal volume of each bacterial isolate suspension was mixed with a two fold serial dilution of the lectin in a microtitre plate and incubated at room temperature for 1 h. The bacterial agglutination titer was expressed as the reciprocal of the highest dilution giving a visible agglutination upon illumination of the microtitre plates (Dutta et al, 2005;Tasumi et al, 2004).…”

“…From the literature, there is growing evidence that lectins from the skin mucus of fish have the ability to agglutinate, opsonize and/or suppresses microbial growth (Suzuki et al, 2003;Tasumi et al, 2004;Dutta et al, 2005;Tsutsui et al, 2006bTsutsui et al, , 2007Argayosa et al, 2011). Antimicrobial activity of epidermal mucus extracts against a broad range of microbial pathogens was observed by Hellio et al (2002).…”

“…The unique properties of animal lectins allow them to play important role in biological processes like detection of cell-surface carbohydrates, signal-transduction, metastasis and apoptosis, opsonization, agglutination and proliferation (Tasumi et al, 2002;Argayosa et al, 2011). Lectins have a significant role in the immune responses of the host (Dutta et al, 2005). They bind specifically to the carbohydrate molecules expresses on the pathogens and help in their rapid clearance by enhancing opsonization and phagocytosis and also by increasing the oxidative burst activities (Tateno et al, 2002).…”

“…Good number of lectins has been reported from fish and has been characterized in terms of agglutination activities, carbohydrate specificities and involvement in the immune response of the fishes (Dutta et al, 2005;Tsutsui et al, 2007;Argayosa et al, 2011;Odekanyin and Kuku, 2014). Res.…”