Characterization of a Functional Soluble Form of a Brassica napus Membrane-Anchored Endo-1,4-beta -Glucanase Heterologously Expressed in Pichia pastoris

Molhoj, M.

doi:10.1104/pp.127.2.674

Cited by 22 publications

(35 citation statements)

References 30 publications

(41 reference statements)

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“…KOR1 is thought to be required for cellulose synthesis acting at the plasma membrane–cell wall interface in plants and other organisms such as bacteria [3], [4], however the precise role of KORRIGAN in cellulose biosynthesis is still unknown. KORRIGAN is suggested to have a proof-reading activity and relieve stress generated during the assembly of glucan chains in cellulose microfibrils by means of hydrolyzing disordered amorphous cellulose [5]. The enzyme heterologously produced in Pichia pastoris cleaves non substituted but non-crystalline 1,4-β–linked glucan chains and shows no activity against xyloglucans [3], [6], [7].…”

Section: Introductionmentioning

confidence: 99%

KORRIGAN1 Interacts Specifically with Integral Components of the Cellulose Synthase Machinery

et al. 2014

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Cellulose is synthesized by the so called rosette protein complex and the catalytic subunits of this complex are the cellulose synthases (CESAs). It is thought that the rosette complexes in the primary and secondary cell walls each contains at least three different non-redundant cellulose synthases. In addition to the CESA proteins, cellulose biosynthesis almost certainly requires the action of other proteins, although few have been identified and little is known about the biochemical role of those that have been identified. One of these proteins is KORRIGAN (KOR1). Mutant analysis of this protein in Arabidopsis thaliana showed altered cellulose content in both the primary and secondary cell wall. KOR1 is thought to be required for cellulose synthesis acting as a cellulase at the plasma membrane–cell wall interface. KOR1 has recently been shown to interact with the primary cellulose synthase rosette complex however direct interaction with that of the secondary cell wall has never been demonstrated. Using various methods, both in vitro and in planta, it was shown that KOR1 interacts specifically with only two of the secondary CESA proteins. The KOR1 protein domain(s) involved in the interaction with the CESA proteins were also identified by analyzing the interaction of truncated forms of KOR1 with CESA proteins. The KOR1 transmembrane domain has shown to be required for the interaction between KOR1 and the different CESAs, as well as for higher oligomer formation of KOR1.

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Section: Introductionmentioning

confidence: 99%

KORRIGAN1 Interacts Specifically with Integral Components of the Cellulose Synthase Machinery

et al. 2014

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“…It is worth noting that the apparent molecular mass of the reacting protein band is between 80 and 100 kD and the predicted molecular weight of aspen KOR based on the amino acid sequence is only about 68 kD. This observation suggests that aspen KOR proteins that are similar to Arabidopsis KOR proteins can be detected using Arabidopsis KOR antibodies, and they might be undergoing post-translational modiWcations similar to rape KOR (Molhoj et al 2001). As stated before, aspen KOR protein has eight such predicted N-glycosylation sites.…”

Section: Immunological Studies Bolster Results From Rt-pcr and In Sitmentioning

confidence: 91%

Xylem-specific and tension stress-responsive coexpression of KORRIGAN endoglucanase and three secondary wall-associated cellulose synthase genes in aspen trees

Bhandari

Fujino

Thammanagowda

et al. 2006

Planta

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In nature, angiosperm trees develop tension wood on the upper side of their leaning trunks and drooping branches. Development of tension wood is one of the straightening mechanisms by which trees counteract leaning or bending of stem and resume upward growth. Tension wood is characterized by the development of a highly crystalline cellulose-enriched gelatinous layer next to the lumen of the tension wood fibers. Thus experimental induction of tension wood provides a system to understand the process of cellulose biosynthesis in trees. Since KORRIGAN endoglucanases (KOR) appear to play an important role in cellulose biosynthesis in Arabidopsis, we cloned PtrKOR, a full-length KOR cDNA from aspen xylem. Using RT-PCR, in situ hybridization, and tissue-print assays, we show that PtrKOR gene expression is significantly elevated on the upper side of the bent aspen stem in response to tension stress while KOR expression is significantly suppressed on the opposite side experiencing compression stress. Moreover, three previously reported aspen cellulose synthase genes, namely, PtrCesA1, PtrCesA2, and PtrCesA3 that are closely associated with secondary cell wall development in the xylem cells exhibited similar tension stress-responsive behavior. Our results suggest that coexpression of these four proteins is important for the biosynthesis of highly crystalline cellulose typically present in tension wood fibers. Their simultaneous genetic manipulation may lead to industrially relevant improvement of cellulose in transgenic crops and trees.

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“…The endo-b-1,4-D-glucanase can hydrolyze b-1,4-linkages behind unsubstituted glucose residues and is involved in the formation of cell wall structure (Molhoj et al, 2001b). Mutation of this gene disrupted cell wall and affected the plant growth.…”

Section: Discussionmentioning

confidence: 99%

OsGLU1, A Putative Membrane-bound Endo-1,4-ß-D-glucanase from Rice, Affects Plant Internode Elongation

Zhou

Cao

et al. 2006

Plant Mol Biol

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A dwarf mutant glu was identified from screening of T-DNA tagged rice population. Genetic analysis of the T1 generation of glu revealed that a segregation ratio of wild-type:dwarf phenotype was 3:1, suggesting that the mutated phenotype was controlled by a single recessive nuclear locus. The mutated gene OsGLU1, identified by Tail-PCR, encodes a putative membrane-bound endo-1,4-beta-D-glucanase, which is highly conserved between mono- and dicotyledonous plants. Mutation of OsGLU1 resulted in a reduction in cell elongation, and a decrease in cellulose content but an increase in pectin content, suggesting that OsGLU1 affects the internode elongation and cell wall components of rice plants. Transgenic glu mutants harboring the OsGLU1 gene complemented the mutation and displayed the wild-type phenotype. In addition, OsGLU1 RNAi plants showed similar phenotype as the glu mutant has. These results indicate that OsGLU1 plays important roles in plant cell growth. Gibberellins and brassinosteroids induced OsGLU1 expression. In rice genome, endo-1,4-beta-D-glucanases form a multiple gene family with 15 members, and each may have a distinct expression pattern in different organs. These results indicate that endo-1,4-beta-D-glucanases may play diverse roles in growth and developmental process of rice plants.

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Characterization of a Functional Soluble Form of a Brassica napus Membrane-Anchored Endo-1,4-beta -Glucanase Heterologously Expressed in Pichia pastoris

Cited by 22 publications

References 30 publications

KORRIGAN1 Interacts Specifically with Integral Components of the Cellulose Synthase Machinery

KORRIGAN1 Interacts Specifically with Integral Components of the Cellulose Synthase Machinery

Xylem-specific and tension stress-responsive coexpression of KORRIGAN endoglucanase and three secondary wall-associated cellulose synthase genes in aspen trees

OsGLU1, A Putative Membrane-bound Endo-1,4-ß-D-glucanase from Rice, Affects Plant Internode Elongation

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