Characterization of a feline sarcoma virus-coded antigen (FOCMA-S) by radioimmunoassay.

Sherr, Charles J.; Todaro, George J.; Sliski, Ann; Essex, Max

doi:10.1073/pnas.75.9.4489

Cited by 18 publications

(11 citation statements)

References 24 publications

(23 reference statements)

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“…Such peptides could be generally represented near the aminotermini of leukemia viral envelope glycoproteins but might also appear at the aminoterminus of the MSV src product and at the carboxytermini of gag-x polyproteins. This hypothesis, although speculative, is consistent with observations showing that gag-x polyproteins are associated with the plasma membranes of transformed cells (37,39,53,54). The ability to identify putative "transformation-specific proteins" and to sequence those portions of the DNA proviruses which encode them now provides the first opportunity to approach these questions directly.…”

Section: Cloning Of Fesv and Felv Linear Dnasupporting

confidence: 72%

“…Indeed, cells nonproductively transformed by the ST strain produce p15, p12, some p30, but no plO antigens (25,30). The p15 and p12 antigens encoded by FeSV are synthesized as part of a high-molecular-weight gag-x polyprotein (25,30,36,37,43). In the ST strain, the apparent molecular weight of this protein is -78,000 (S. Ruscetti, L. Turek, and C. J. Sherr, unpublished data), which is expected to contain a 51,000 "X"-moiety of non-gag origin.…”

Section: Cloning Of Fesv and Felv Linear Dnamentioning

confidence: 99%

See 1 more Smart Citation

Molecular cloning of Snyder-Theilen feline leukemia and sarcoma viruses: comparative studies of feline sarcoma virus with its natural helper virus and with Moloney murine sarcoma virus

Sherr¹,

Fedele²,

Oskarsson³

et al. 1980

J Virol

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100

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Extrachromosomal DNA obtained from mink cells acutely infected with the Snyder-Theilen (ST) strain of feline sarcoma virus (feline leukemia virus) [FeSV(FeLV)] was fractionated electrophoretically, and samples enriched for FeLV and FeSV linear intermediates were digested with EcoRI and cloned in lambda phage. Hybrid phages were isolated containing either FeSV or FeLV DNA "inserts" and were characterized by restriction enzyme analysis, R-looping with purified 26 to 32S viral RNA, and heteroduplex formation. The recombinant phages (designated lambda FeSV and lambda FeLV) contain all of the genetic information represented in FeSV and FeLV RNA genomes but lack one extended terminally redundant sequence of 750 bases which appears once at each end of parental linear DNA intermediates. Restriction enzyme and heteroduplex analyses confirmed that sequences unique to FeSV (src sequences) are located at the center of the FeSV genome and are approximately 1.5 kilobase pairs in length. With respect to the 5'-3' orientation of genes in viral RNA, the order of genes in the FeSV genome is 5'-gag-src-env-c region-3'; only 0.9 kilobase pairs of gag and 0.6 kilobase pairs of env-derived FeLV sequences are represented in ST FeSV. Heteroduplex analyses between lambda FeSV or lambda FeLV DNA and Moloney murine sarcoma virus DNA (strain m1) were performed under conditions of reduced stringency to demonstrate limited regions of base pair homology. Two such regions were identified: the first occurs at the extreme 5' end of the leukemia and both sarcoma viral genomes, whereas the second corresponds to a 5' segment of leukemia virus "env" sequences conserved in both sarcoma viruses. The latter sequences are localized at the 3' end of FeSV src and at the 5' end of murine sarcoma virus src and could possibly correspond to regions of helper virus genomes that are required for retroviral transforming functions.

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Section: Cloning Of Fesv and Felv Linear Dnasupporting

confidence: 72%

Section: Cloning Of Fesv and Felv Linear Dnamentioning

confidence: 99%

Molecular cloning of Snyder-Theilen feline leukemia and sarcoma viruses: comparative studies of feline sarcoma virus with its natural helper virus and with Moloney murine sarcoma virus

Sherr¹,

Fedele²,

Oskarsson³

et al. 1980

J Virol

Self Cite

100

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show abstract

“…The weight of indirect 3978 Medical Sciences: Reynolds et al evidence would seem to favor its identity as a src gene product, on the basis of analogy to findings recently obtained in studies of other RNA tumor viruses. For instance, in the feline system, a 110,000-to 130,000-Mr translational product of the FeSV genome containing FeLV p15 and p12 linked to a 60,000-Mr nonstructural component has been shown to react with tumor-specific antisera (2,33,34). An avian RNA tumor virus-(MC29) coded polyprotein consisting of two avian leukemia virus gag gene proteins, p19 and p27, linked to a 60,000-Mr nonstructural protein has also been described (39).…”

Section: Discussionmentioning

confidence: 99%

Cells nonproductively transformed by Abelson murine leukemia virus express a high molecular weight polyprotein containing structural and nonstructural components.

Reynolds¹,

Sacks²,

Deobagkar³

et al. 1978

Proc. Natl. Acad. Sci. U.S.A.

114

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Cell clones nonproductively transformed by the replication-defective Abelson strain of murine leukemia virus (AbLV) were analyzed for type C viral antigen expression by competition immunoassay. AbLV-transformed mink non-producer lines were found to express a 110,000- to 130,000-molecular weight polyprotein containing murine leukemia virus gag proteins p15 and p12 covalently linked to nonstructural AbLV-coded component(s) of around 80,000-100,000 molecular weight. This polyprotein lacked detectable antigenic cross-reactivity with other virion-coded gag gene proteins such as p30, p10, the viral reverse transcriptase (RNA-dependent DNA polymerase), or the major viral envelope glycoprotein, gp70. By analogy to earlier data on feline and avian sarcoma viruses, these results suggest that a portion of this polyprotein might represent the AbLV src gene product and that in translation it is initially linked in precursor form to gag structural proteins. Superinfection of mink cells nonproductively transformed by AbLV--with either a wild mouse amphotropic type C virus isolate, 4070-A, or with the endogenous cat virus, RD114--led to production of pseudotype virus containing high concentrations of the AbLV-coded precursor polyprotein.

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“…When the defective FeSV genome is 'rescued' by infection of FeSV-transformed cells with heterologous helper viruses, extracellular type C particles contain the p85 gag-X polyprotein [70]. Partial purification of the polyprotein from such 'pseudotype' virions has enabled the development of a radioimmunoassay for FOCMA [71]. Presumably, the gag moiety facilitates the selective packaging of the gag-FOCMA polyprotein into extracellular particles since p60 FOCMA has not been detected in extracellular virions [70].…”

Section: The Search For 'Transforming' Proteinsmentioning

confidence: 98%

Type C viruses: Natural derivatives of cellular genes involved in malignant transformation

Sherr

Todaro

1979

Springer Semin Immunopathol

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Characterization of a feline sarcoma virus-coded antigen (FOCMA-S) by radioimmunoassay.

Abstract: A radioimmunoassay has been developed that detects a unique antigen encoded by the genome of the feline sarcoma virus (FeSV)

Cited by 18 publications

References 24 publications

Molecular cloning of Snyder-Theilen feline leukemia and sarcoma viruses: comparative studies of feline sarcoma virus with its natural helper virus and with Moloney murine sarcoma virus

Molecular cloning of Snyder-Theilen feline leukemia and sarcoma viruses: comparative studies of feline sarcoma virus with its natural helper virus and with Moloney murine sarcoma virus

Cells nonproductively transformed by Abelson murine leukemia virus express a high molecular weight polyprotein containing structural and nonstructural components.

Type C viruses: Natural derivatives of cellular genes involved in malignant transformation

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