Characterization of a fatty acid-binding protein from the Pacific oyster (Crassostrea gigas): pharmaceutical and toxicological implications

Tisca, Juliana F.; Santos, Karin dos; Pessati, Tomás B.; Zacchi, Flávia L.; Soares, Fabíola S.; Oliveira, Vanessa A.; Bebianno, Maria João; Bainy, Afonso Celso Dias; Razzera, Guilherme

doi:10.1007/s11356-021-12645-y

Cited by 4 publications

(1 citation statement)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, we found that rSmFABP appears to form relatively stable binding forms with palmitic acid, although the stability of the conformational change is relatively limited. Different fatty acids have different affinities for FABPs [ 50 , 51 ]; rSmFABP seems to bind more stably to palmitic acid than oleic acid, which is inconsistent with previous studies [ 52 ]. Additionally, to explore whether SmFABP participates in lipid-metabolism-related activities, a fatty acid starvation induction test was performed in this study.…”

Section: Discussionmentioning

confidence: 89%

Molecular Characteristics of the Fatty-Acid-Binding Protein (FABP) Family in Spirometra mansoni―A Neglected Medical Tapeworm

Liu,

Gao,

Wang

et al. 2023

Animals

View full text Add to dashboard Cite

The plerocercoid larva of the tapeworm Spirometra mansoni can parasitize humans and animals, causing serious parasitic zoonosis. The molecular characteristics and adaptive parasitism mechanism of Spirometra tapeworms are still unknown. In this study, 11 new members of the fatty-acid-binding protein (FABP) family were characterized in S. mansoni. A clustering analysis showed 11 SmFABPs arranged into two groups, and motif patterns within each group had similar organizations. RT–qPCR showed that SmFABPs were highly expressed in the adult stage, especially in gravid proglottid. A high genetic diversity of SmFABPs and relative conservation of FABPs in medical platyhelminthes were observed in the phylogenetic analysis. Immunolocalization revealed that natural SmFABP is mainly located in the tegument and parenchymal tissue of the plerocercoid and the uterus, genital pores, and cortex of adult worms. rSmFABP can build a more stable holo form when binding with palmitic acid to protect the hydrolytic sites of the protein. A fatty acid starvation induction test suggested that SmFABP might be involved in fatty acid absorption, transport, and metabolism in S. mansoni. The findings in this study will lay the foundation to better explore the underlying mechanisms of FABPs involved in Spirometra tapeworms as well as related taxa.

show abstract

Section: Discussionmentioning

confidence: 89%