Characterization of a D2-like dopamine receptor (AmDOP3) in honey bee, Apis mellifera

Beggs, Kyle T.; Hamilton, Ingrid S.; Kurshan, Peri T.; Mustard, Julie A.; Mercer, Alison R.

doi:10.1016/j.ibmb.2005.03.005

Cited by 88 publications

(98 citation statements)

References 58 publications

(80 reference statements)

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“…This is contrary to previous studies on the fly and bee orthologs of D 2αPan , which show that when these receptors are expressed in HEK cell lines, exposure to DA produces a decrease in cAMP (Beggs et al, 2005;Hearn et al, 2002). It is not clear whether the difference lies in the cell lines or the arthropod D 2 receptors.…”

Section: The Da-induced Increase In [Camp]i Is Mediated By Gi/o Proteinscontrasting

confidence: 89%

“…As previously stated, when expressed in HEK293 cells, the lobster versus fruit fly and honeybee orthologs of the D 2 receptor produce opposite changes in cAMP levels in response to DA: the lobster D 2 receptor positively couples with cAMP (Figure 3) while the fly and bee orthologs of the D 2 receptor negatively couple with cAMP (Beggs et al, 2005;Hearn et al, 2002). We predicted that if the cellular background determines whether D 2 receptors positively or negatively couple with cAMP, then expressing the honeybee ortholog of the D 2 receptor, AmDop3, in our parental HEK293 cell line should produce an increase, rather than the previously described decrease in cAMP.…”

Section: The Intracellular Milieu Determines Whether Arthropod D 2 Rementioning

confidence: 54%

“…Τhe D 2αPan receptor is orthologous to the Drosophila receptor DD2R (Hearn et al, 2002) and the Apis receptor AmDop3 (Beggs et al, 2005). A BLAST against the Homo sapien Reference Proteins database showed that the D 2αPan receptor was most homologous to the long form of the human D 2 receptor (NP_000786) with an E value of 3e-58.…”

Section: Dars Are Conserved Across Speciesmentioning

confidence: 99%

“…In addition to DA, tyramine stimulates the DD2R and AmDop3 receptors, and DD2R responds to serotonin (5-HT) (Hearn et al, 2002;Beggs et al, 2005). Gα i/o proteins are known to decrease AC activity and reduce [cAMP]i.…”

Section: α1pan Couples With Multiple Members Of the Gi/o Family Imentioning

confidence: 99%

“…There are three arthropod DARs: two type-1 receptors and one type-2 receptor (Gotzes et al, 1994;Feng et al, 1996;Han et al, 1996;Hearn et al, 2002;Blenau et al, 1998;Beggs et al, 2005;Mustard et al, 2003). A fourth arthropod receptor that responds to DA with an increase in cAMP has been cloned, but it is primarily activated by ecdysteroids and does not appear to belong to the DAR family (Srivastava et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Arthropod D2 receptors positively couple with cAMP through the Gi/o protein family

Clark

Baro

2007

Comparative Biochemistry and Physiology Part B: Biochemistry an

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The pyloric network is an important model system for understanding neuromodulation of rhythmic motor behaviors like breathing or walking. Dopamine (DA) differentially modulates neurons within the pyloric network. However, while the electrophysiological actions of DA have been well characterized, nothing is known about the signaling events that mediate its effects. We have begun a molecular characterization of DA receptors (DARs) in this invertebrate system. Here, we describe the cloning and characterization of the lobster D 2 receptor, D 2αPan . We found that when expressed in HEK cells, the D 2αPan receptor is activated by DA, but not other monoamines endogenous to the lobster nervous system. This receptor positively couples with cAMP through multiple Gi/o proteins via two discrete pathways: 1) a Gα mediated inhibition of adenylyl cyclase (AC), leading to a decrease in cAMP and 2) a Gβγ mediated activation of Phospholipase Cβ (PLCβ), leading to an increase in cAMP. Alternate splicing alters the potency and efficacy of the receptor, but does not affect monoamine specificity. Finally, we show that arthropod D 2 receptor coupling with cAMP varies with the cellular milieu.

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Section: The Da-induced Increase In [Camp]i Is Mediated By Gi/o Proteinscontrasting

confidence: 89%

Section: The Intracellular Milieu Determines Whether Arthropod D 2 Rementioning

confidence: 54%

Section: Dars Are Conserved Across Speciesmentioning

confidence: 99%

Section: α1pan Couples With Multiple Members Of the Gi/o Family Imentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Arthropod D2 receptors positively couple with cAMP through the Gi/o protein family

Clark

Baro

2007

Comparative Biochemistry and Physiology Part B: Biochemistry an

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Molecular biology of the invertebrate dopamine receptors

Mustard

Beggs

Mercer

2005

Arch Insect Biochem Physiol

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102

132

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Dopamine is found in the nervous systems of both vertebrates and invertebrates. However, the specific actions of dopamine depend on the dopamine receptor type that is expressed in the target cell. As in mammals, different subtypes of dopamine receptors have been cloned and characterized from invertebrates, and these receptor subtypes have different structural and functional properties. Understanding how these receptors respond to dopamine and in which cells each receptor type is expressed is key to our understanding of the role of dopamine signaling. Comparison of the amino acid sequences and experimentally determined functional properties suggest that there are at least three distinct types of dopamine receptors in invertebrates. This review focuses on invertebrate dopamine receptors for which the genes have been isolated and identified, and examines our current knowledge of the functional and structural properties of these receptors, and their pharmacology and expression.

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Locomotor activity is regulated by D2‐like receptors in Drosophila: An anatomic and functional analysis

Draper

Kurshan

McBride

et al. 2007

Developmental Neurobiology

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111

101

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In mammals, dopamine 2-like receptors are expressed in distinct pathways within the central nervous system, as well as in peripheral tissues. Selected neuronal D2-like receptors play a critical role in modulating locomotor activity and, as such, represent an important therapeutic target (e.g. in Parkinson's disease). Previous studies have established that proteins required for dopamine (DA) neurotransmission are highly conserved between mammals and the fruit fly Drosophila melanogaster. These include a fly dopamine 2-like receptor (DD2R; Hearn et al. PNAS 2002 99(22):14554) that has structural and pharmacologic similarity to the human D2-like (D2R). In the current study, we define the spatial expression pattern of DD2R, and functionally characterize flies with reduced DD2 receptor levels. We show that DD2R is expressed in the larval and adult nervous systems, in cell groups that include the Ap-let cohort of peptidergic neurons, as well as in peripheral tissues including the gut and Malpighian tubules. To examine DD2R function in vivo, we generated RNA-interference (RNAi) flies with reduced DD2R expression. Behavioral analysis revealed that these flies show significantly decreased locomotor activity, similar to the phenotype observed in mammals with reduced D2R expression. The fly RNAi phenotype can be rescued by administration of the DD2R synthetic agonist bromocriptine, indicating specificity for the RNAi effect. These results suggest Drosophila as a useful system for future studies aimed at identifying modifiers of dopaminergic signaling/locomotor function.

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Characterization of a D2-like dopamine receptor (AmDOP3) in honey bee, Apis mellifera

Cited by 88 publications

References 58 publications

Arthropod D2 receptors positively couple with cAMP through the Gi/o protein family

Arthropod D2 receptors positively couple with cAMP through the Gi/o protein family

Molecular biology of the invertebrate dopamine receptors

Locomotor activity is regulated by D2‐like receptors in Drosophila: An anatomic and functional analysis

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