Characterization of a continuous T-cell line susceptible to the cytopathic effects of the acquired immunodeficiency syndrome (AIDS)-associated retrovirus.

Abstract

We have developed a continuous human T-cell line (A3.01) for the study of acquired immunodeficiency syndrome (AIDS)-associated retrovirus that mimics normal peripheral blood lymphocytes in susceptibility to viral cytopathic effect without the need for cell activation or conditioned medium. Following infection, substantial quantities of virus are produced during a 3- to 5-day period; the associated killing of cells can be monitored in a microtiter assay as a function of virus input. Southern blot hybridization of infected cellular DNAs indicated that no gross alteration occurred in the restriction maps of the proviral DNA during the transfer of virus to and its passage in A3.01 cells. This cell system offers an alternative to other AIDS retrovirus cell systems because it permits the monitoring of viral cytopathic effects.

Phenotypic variation in the response to the human immunodeficiency virus among derivatives of the CEM T and WIL-2 B cell lines.

“…For example, X50-7, an EBVtransformed B cell line that expresses CD4 on 60-70% of cells, exhibits a cytopathic effect within 15 d (46) . A3 .01, a HAT sensitive derivative of CEM, showed maximal RT activity at 10 d after infection, coincident with cell death (47), which is similar to the time course we observe with our CEM4 clone. The ATH8 T cell line exhibits a cytopathic effect within 4 d of infection by HTLVIIB, but it requires IL-2 for growth and is HTLVI transformed (48).…”

Establishment of novel cell lines latently infected with human immunodeficiency virus 1

“…We established latently infected NCHA 1, 2 cell lines by limiting dilution cloning after the infection of A3.01 cells with HIV-1 pNL4-3 strain. The A3.01 cells are human T-cell line developed for the study of AIDS-associated retrovirus, which mimic normal peripheral blood lymphocytes in susceptibility to the viral cytopathic effect without cell activation or conditioned medium (Folks et al, 1985). As HIV-1 integration into the host chromosome is a critical step for HIV-1 life cycle, HIV-1 integration assay is very important for the understanding of HIV-1 latency mechanisms (Vatakis et al, 2007).…”

A specific defect in CD3 gamma-chain gene transcription results in loss of T-cell receptor/CD3 expression late after human immunodeficiency virus infection of a CD4+ T-cell line.

“…1 and 2) can remain asymptomatic for years before the onset of acquired immunodeficiency syndrome (AIDS), a disease characterized by the gradual loss of CD4+ T-cell-dependent immune responses. Most in vitro studies of HIV infection using human CD4+ T-cell lines or normal peripheral blood lymphocytes have demonstrated the development of multinucleated syncytial cells, severe downmodulation of CD4 on the infected cell surface, and cytopathic death (3)(4)(5)(6)(7). In some cases, infected cells survive infection, but they no longer express viral proteins or CD4 (8).…”