Characterization of a colorectal cancer migration and autophagy-related microRNA miR-338-5p and its target gene PIK3C3

Ju, J.; Huang, Ching Tang; Lan, Sheng Hui; Wang, Ting Huei; Lin, Peng; Lee, Jheng Chang; Tian, Yu; Liu, Hsiao Sheng

doi:10.1016/j.bgm.2013.07.006

Cited by 19 publications

(11 citation statements)

References 19 publications

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“…The target genes are participated in diverse pathophysiological processes including cell organ size, cell differentiation, cell proliferation and cell migration, which may implicated in the pathogenesis of sIGUR. DEMs, including miR-373 ( 27 ), miR-338-5p ( 34 ), miR-590-5p ( 30 , 35 , 36 ), miR-623 ( 37 ) and miR-370-3p ( 31 ), have been reported involved in regulating the proliferation, migration and invasion of cancer cells, which was consistent with our findings. Further studies on the expression pattern and function of these target genes may advance our understanding of the implications of theses DEMs in sIGUR pathogenesis.…”

Section: Discussionsupporting

confidence: 92%

MicroRNA expression profiles and networks in placentas complicated with selective intrauterine growth restriction

Wen

Chen

et al. 2017

Molecular Medicine Reports

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The microRNA (miRNA) profiles of placentas complicated with selective intrauterine growth restriction (sIUGR) are unknown. In the present study, the sIUGR-associated placental miRNA expression was investigated using microarray and confirmatory reverse transcriptase-quantitative polymerase chain reaction studies. Placenta samples around the individual insertion region for each umbilical cord were collected from monochorionic twins complicated with (n=17) or without sIUGR (control, n=16). miRNA profile analysis was performed on two sIUGR cases and one control using an Affymetrix microRNA 4.0 Array system. A total of 14 miRNAs were identified to be specifically differentially expressed (7 upregulated and 7 downregulated) among larger twins of sIUGR cases compared with smaller twins of sIUGR cases. The target genes of the identified miRNAs participate in organ size, cell differentiation, cell proliferation and migration. In addition, according to the miRNA-pathway network analysis, key miRNAs and pathways (transforming growth factor-β, mitogen-activated protein kinase and Wnt) were identified to be associated with the pathogenesis of sIUGR. To the best of our knowledge, the results of the current study have provided the most complete miRNA profiles and the most detailed miRNA regulatory networks of placental tissues complicated with sIUGR.

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Section: Discussionsupporting

confidence: 92%

MicroRNA expression profiles and networks in placentas complicated with selective intrauterine growth restriction

Wen

Chen

et al. 2017

Molecular Medicine Reports

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“…MiR-338-5p can be spliced from pre-miR-338 and miR-338-5p was upregulated similar to that observed in pre-miR-338-transfected cancer cells; therefore, it is reasonable that miR-338-5p participates in cancer progression. In previous studies, to the best of our knowledge, the function of miR-338-5p in tumorigenesis was reported in only one study that regarded miR-338-5p as an oncomir inducing cell migration by suppressing PIK3C3 expression and autophagy in colorectal cancer [47]. Contradictory to previous observations, we further confirmed that miR-338-5p is downregulated in the GC tissues and believed that miR-338-5p functions as a tumor suppressor.…”

Section: Discussionsupporting

confidence: 88%

MECP2 promotes the growth of gastric cancer cells by suppressing miR-338-mediated antiproliferative effect

Tong

Zhao

et al. 2016

Oncotarget

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The methyl-CpG-binding protein 2 (MECP2), a transcriptional suppressor, is involved in gene regulation by binding to methylated promoters. We found that MECP2 is overexpressed in gastric cancer (GC), and that Mecp2 knockdown affects the growth of GC cells both in vitro and in vivo. MECP2 can directly bind to the methylated-CpG island of miR-338 promoter and suppress the expression of two mature microRNAs, namely, miR-338-3p and miR-338-5p. Furthermore, miR-338-5p can suppress GC cell growth by targeting BMI1 (B lymphoma Mo-MLV insertion region 1 homolog). We additionally found that decreased miR-338-5p expression in GC tissues, relative to normal tissues, was significantly negatively correlated with increased BMI1 expression. Silencing MECP2 can indirectly lead to reduced expression of P-REX2, which has been identified as the miR-338-3p target, as well as BMI1 and increasing expression of P16 or P21 both in vitro and in vivo. Altogether, our results indicate that MECP2 promote the proliferation of GC cells via miR-338 (miR-338-3p and miR-338-5p)-mediated antitumor and gene regulatory effect.

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“…24,25 MiR-338-5p induces the migration and metastasis of CRC cells by suppressing autophagy and its target gene, phosphatidylinositol 3-kinase catalytic subunit type 3 (PIK3C3). 26 Researchers have found that dysregulated miRNAs are shed from diseased tissues into the circulation, and circulating miRNAs are protected by binding to Argonaute proteins and through encapsulation by high density lipoprotein particles, exosomes and microvesicles, which make them difficult to be degraded by RNase. 27,28 Therefore, serum miR-338-5p can be detected.…”

Section: Discussionmentioning

confidence: 99%

Circulating miR‐338‐5p is a potential diagnostic biomarker in colorectal cancer

Bilegsaikhan

Liu

Shen

et al. 2018

J of Digest Diseases

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Characterization of a colorectal cancer migration and autophagy-related microRNA miR-338-5p and its target gene PIK3C3

Cited by 19 publications

References 19 publications

MicroRNA expression profiles and networks in placentas complicated with selective intrauterine growth restriction

MicroRNA expression profiles and networks in placentas complicated with selective intrauterine growth restriction

MECP2 promotes the growth of gastric cancer cells by suppressing miR-338-mediated antiproliferative effect

Circulating miR‐338‐5p is a potential diagnostic biomarker in colorectal cancer

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